the pdf - University of British Columbia
... ~250kDa, have both been found to inhibit neurite outgrowth in vitro (Schwab & Caroni, 1988; Caroni & Schwab, 1988). As well, in vivo studies have successfully blocked the neurite inhibiting properties of these two proteins and enhanced axonal regeneration after CNS injury (Schnell & Schwab, 1990). ...
... ~250kDa, have both been found to inhibit neurite outgrowth in vitro (Schwab & Caroni, 1988; Caroni & Schwab, 1988). As well, in vivo studies have successfully blocked the neurite inhibiting properties of these two proteins and enhanced axonal regeneration after CNS injury (Schnell & Schwab, 1990). ...
sample-student-2-Pos.. - NuWrite
... •Prp8 has few characteristic domains, but does contain a variant Jab1/MPN domain. •The Jab1/MPN domain is normally found within deubiquitinating enzymes that remove ubiquitin from proteins marked for destruction. •The non-canonical Jab1/MPN domain of Prp8 does not enable enzyme function, but is stil ...
... •Prp8 has few characteristic domains, but does contain a variant Jab1/MPN domain. •The Jab1/MPN domain is normally found within deubiquitinating enzymes that remove ubiquitin from proteins marked for destruction. •The non-canonical Jab1/MPN domain of Prp8 does not enable enzyme function, but is stil ...
Distributed Representations for Biological Sequence Analysis
... molecular level. Large scale assignment of proteins to families grew out of the work of Dayhoff [7]. Sequence homology is regarded as a direct reflection of evolutionary relatedness, and members of the same protein family may also exhibit similar secondary structure through common functional units c ...
... molecular level. Large scale assignment of proteins to families grew out of the work of Dayhoff [7]. Sequence homology is regarded as a direct reflection of evolutionary relatedness, and members of the same protein family may also exhibit similar secondary structure through common functional units c ...
Ro52: Structure and interactions of constructs of RING and B-box
... In this thesis three constructs of two domains from Ro52 (RING and B-box) is investigated by circular dichroism (CD), nuclear magnetic resonance (NMR) spectroscopy and auto-ubiquitination assay by Western blot. The goal was to gain deeper insight in structural and functional properties of these doma ...
... In this thesis three constructs of two domains from Ro52 (RING and B-box) is investigated by circular dichroism (CD), nuclear magnetic resonance (NMR) spectroscopy and auto-ubiquitination assay by Western blot. The goal was to gain deeper insight in structural and functional properties of these doma ...
No Slide Title
... banding" in sucrose density gradients. Comparison with the amino-acid sequences of these proteins with Class II histocompatability DR proteins proved that "the DR alpha and beta chains appeared to be identical to the p34-36 and p30-32 proteins respectively” Cellular origin also acknowledged by other ...
... banding" in sucrose density gradients. Comparison with the amino-acid sequences of these proteins with Class II histocompatability DR proteins proved that "the DR alpha and beta chains appeared to be identical to the p34-36 and p30-32 proteins respectively” Cellular origin also acknowledged by other ...
Plant mitochondria contain the protein translocase subunits TatB
... believe that At5g43680 is a TatB like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in comparison to 10% identity and 19% similarity to EcTatA. Furthermore, a sequence alignment between EcTatB and At5g43680 showed that it ...
... believe that At5g43680 is a TatB like protein. This is also supported by basic sequence comparisons as At5g43680 shows a 14% identity and 29% similarity to EcTatB in comparison to 10% identity and 19% similarity to EcTatA. Furthermore, a sequence alignment between EcTatB and At5g43680 showed that it ...
A Tertiary Plastid Uses Genes from Two Endosymbionts
... from another secondary alga, resulting in tertiary plastids.9–13 These organisms are not only fascinatingly complex cells, but they also have characteristics that make them useful models to study the effects on the genome of endosymbiosis: the endosymbiotic events took place recently compared to pri ...
... from another secondary alga, resulting in tertiary plastids.9–13 These organisms are not only fascinatingly complex cells, but they also have characteristics that make them useful models to study the effects on the genome of endosymbiosis: the endosymbiotic events took place recently compared to pri ...
Biosynthesis of Protein or Translation
... The amino acids have no direct affinity for mRNA, so tRNA act as an adapter molecule, which recognizes an amino acid on one end and its corresponding codon on the other, is required for translation. ...
... The amino acids have no direct affinity for mRNA, so tRNA act as an adapter molecule, which recognizes an amino acid on one end and its corresponding codon on the other, is required for translation. ...
Amino Acid Analyzer
... or other highly molecular compounds have to be removed so as not to obstruct separation column and this could be done by • Acid precipitation (by using Sulfosalisilic acid) ...
... or other highly molecular compounds have to be removed so as not to obstruct separation column and this could be done by • Acid precipitation (by using Sulfosalisilic acid) ...
Drosophila ventral furrow morphogenesis: a
... obtain relatively pure populations of ventral and lateral cells, genetically ventralized and lateralized embryos were used (St. Johnston and Nüsslein-Volhard, 1992; Ferguson and Anderson, 1992). Females that were heterozygous for Tl10b (Tl10b/+) produced ventralized embryos. Females that were hetero ...
... obtain relatively pure populations of ventral and lateral cells, genetically ventralized and lateralized embryos were used (St. Johnston and Nüsslein-Volhard, 1992; Ferguson and Anderson, 1992). Females that were heterozygous for Tl10b (Tl10b/+) produced ventralized embryos. Females that were hetero ...
Amino acids
... nonpolar amino acids tend to cluster together in the interior of the protein. • The nonpolar R-group fill up the interior of the folded protein and help give it its 3D shape. • In proteins located in hydrophobic environment, such as a membrane, the nonpolar R-groups are found on the outside surface ...
... nonpolar amino acids tend to cluster together in the interior of the protein. • The nonpolar R-group fill up the interior of the folded protein and help give it its 3D shape. • In proteins located in hydrophobic environment, such as a membrane, the nonpolar R-groups are found on the outside surface ...
北京聚合美生物科技有限公司 Mei5 Biotechnology, Co., Ltd M5 GFP
... IP of extracts from COS7 cells transfected with a GFP-tagged protein using GFP rabbit polyclonal antibody (5μl ,lane 3) or using normal rabbit IgG(lane 2) as control,followed by Western blot using GFP (7G9) Mouse mAb(dilution:1:5000).Lane 1 was loaded with 10 μg of COS7 cell lysate over-expressing a ...
... IP of extracts from COS7 cells transfected with a GFP-tagged protein using GFP rabbit polyclonal antibody (5μl ,lane 3) or using normal rabbit IgG(lane 2) as control,followed by Western blot using GFP (7G9) Mouse mAb(dilution:1:5000).Lane 1 was loaded with 10 μg of COS7 cell lysate over-expressing a ...
Amino acids
... group that can participate in hydrogen bond formation. • The side chains of asparagine and glutamine each contain a carbonyl group and an amide group, both of which can also participate in hydrogen bonds. ...
... group that can participate in hydrogen bond formation. • The side chains of asparagine and glutamine each contain a carbonyl group and an amide group, both of which can also participate in hydrogen bonds. ...
MND Australia International Research Update December 2016
... gene were the first to be identified in people with MND. These mutations cause the SOD1 protein to be unstable and form aggregates – protein clumps – in MNs. It’s important for cells to be able to remove unstable SOD1 molecules, but in people with SOD1 mutations their MNs become unable to properly e ...
... gene were the first to be identified in people with MND. These mutations cause the SOD1 protein to be unstable and form aggregates – protein clumps – in MNs. It’s important for cells to be able to remove unstable SOD1 molecules, but in people with SOD1 mutations their MNs become unable to properly e ...
The Proteomic Code: a molecular recognition code for proteins
... establish a large 3D structure. This structure has protrusions and cavities, charged and uncharged areas, hydrophobic and hydrophilic patches on its surface, which altogether form a complex 3D pattern of spatial and physico-chemical properties. Two proteins will specifically interact with each other ...
... establish a large 3D structure. This structure has protrusions and cavities, charged and uncharged areas, hydrophobic and hydrophilic patches on its surface, which altogether form a complex 3D pattern of spatial and physico-chemical properties. Two proteins will specifically interact with each other ...
Cofactor requirement of ribosome-inactivating
... linked to a B-chain able to bind to cell surface receptors on target cells. Type 1 RIPs are widely distributed in higher plants and many plants produce several RIPs, similar but not identical to each other (possibly isoenzymes), either present within the same organ or in different organs (reviewed i ...
... linked to a B-chain able to bind to cell surface receptors on target cells. Type 1 RIPs are widely distributed in higher plants and many plants produce several RIPs, similar but not identical to each other (possibly isoenzymes), either present within the same organ or in different organs (reviewed i ...
Atomic model of human Cystic Fibrosis Transmembrane
... Supplementary data 3: Modeling of the NBD1 regulatory insertion. The modeling of the NBD1 regulatory insertion has been performed using as template the experimental 3D structure of the isolated mouse CFTR NBD1 ([2], pdb 1r0z; at left), for which only six amino acids at the centre of the loop (dotte ...
... Supplementary data 3: Modeling of the NBD1 regulatory insertion. The modeling of the NBD1 regulatory insertion has been performed using as template the experimental 3D structure of the isolated mouse CFTR NBD1 ([2], pdb 1r0z; at left), for which only six amino acids at the centre of the loop (dotte ...
PDF + SI - Journal of Cell Science
... Supergroup colour codes are as for Fig. 1; the numerical values at the nodes of the tree and symbols for highly supported nodes are as in Fig. 2. Bacterial species names are shown in black, and archaea species names are shown in grey. The eukaryotes form a distinct and well-supported group, separate ...
... Supergroup colour codes are as for Fig. 1; the numerical values at the nodes of the tree and symbols for highly supported nodes are as in Fig. 2. Bacterial species names are shown in black, and archaea species names are shown in grey. The eukaryotes form a distinct and well-supported group, separate ...
Protein Analysis - GRiSP Research Solutions
... Western blotting is a widely-used analytical technique for the study of proteins. This method, first described by Towbin, et. al1, allows for the detection of a single protein within a mixture of proteins derived from a biological sample. The specificity of Western blotting is achieved by using an a ...
... Western blotting is a widely-used analytical technique for the study of proteins. This method, first described by Towbin, et. al1, allows for the detection of a single protein within a mixture of proteins derived from a biological sample. The specificity of Western blotting is achieved by using an a ...
Assembly of AO and DHAS - Journal of Cell Science
... The data show that the matrix fraction contained virtually all our data is that the dimeric form of DHAS is the major if not the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having sho ...
... The data show that the matrix fraction contained virtually all our data is that the dimeric form of DHAS is the major if not the AO and DHAS from the pellet (Fig. 1C) and no the only species that crosses the peroxisomal membrane. peroxisomal membrane (Fig. 1D). In summary, there is little Having sho ...
galactose specific
... • Acta Cryst. (1999) D55, 1375-1382 • JMB (2000) 302, 1129-1137 • Proteins SFG (2001) 43, 260-270 • JBC (2001) 276, 40734-40739 • Protein Eng. (2001) 14, 857-866 • Acta Cryst. (2001) D57, 1584-1594 • Acta Cryst. (2003) D59, 2254-2256 ...
... • Acta Cryst. (1999) D55, 1375-1382 • JMB (2000) 302, 1129-1137 • Proteins SFG (2001) 43, 260-270 • JBC (2001) 276, 40734-40739 • Protein Eng. (2001) 14, 857-866 • Acta Cryst. (2001) D57, 1584-1594 • Acta Cryst. (2003) D59, 2254-2256 ...
WAVE/Scars in platelets
... phosphorylated upon stimulation by collagen or following crosslinking of CD32, a low-affinity Fc receptor for immunoglobulin G (IgG).16 However, despite the very small size of platelets from patients with WAS, these small platelets undergo shape changes in suspensions and upon surfaces that are simi ...
... phosphorylated upon stimulation by collagen or following crosslinking of CD32, a low-affinity Fc receptor for immunoglobulin G (IgG).16 However, despite the very small size of platelets from patients with WAS, these small platelets undergo shape changes in suspensions and upon surfaces that are simi ...
Protein dynamics and proteolysis in plant vacuoles
... (endosperm, cotyledon mesophyll, embryonic axis) organs are sites of short- or long-term deposition of protein reserves. An extensive proteome analysis of PSV has not yet been conducted but, using immunohistochemistry, many proteins have been found to be localized there. Besides large amounts of pol ...
... (endosperm, cotyledon mesophyll, embryonic axis) organs are sites of short- or long-term deposition of protein reserves. An extensive proteome analysis of PSV has not yet been conducted but, using immunohistochemistry, many proteins have been found to be localized there. Besides large amounts of pol ...
General principles of cellular communication
... receptors (names that variously identify a key component, signal, receptor type or function). These seven pathways are used during development to define the size, shape and other characteristics of an animal. How can so few pathways achieve so much? The answer is that signalling pathways can act tog ...
... receptors (names that variously identify a key component, signal, receptor type or function). These seven pathways are used during development to define the size, shape and other characteristics of an animal. How can so few pathways achieve so much? The answer is that signalling pathways can act tog ...
The Arabidopsis TRM1-TON1 interaction reveals a recruitment
... (Figure 2), plus a few isolated ones. Apart from the six motifs, no significant similarity is detectable between groups. TRMs are rather large (80 kD on average) and charged proteins, covering a large range of pI from 4.3 to 10.6. They often contain a large positively charged domain of 150 to 300 re ...
... (Figure 2), plus a few isolated ones. Apart from the six motifs, no significant similarity is detectable between groups. TRMs are rather large (80 kD on average) and charged proteins, covering a large range of pI from 4.3 to 10.6. They often contain a large positively charged domain of 150 to 300 re ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.