KASH `n Karry: The KASH domain family of cargo
... only, while a complementary protein fragment containing the KASH domain localizes only to apical microtubules.(29) In addition, the Drosophila gene encoding B-type Lamin ...
... only, while a complementary protein fragment containing the KASH domain localizes only to apical microtubules.(29) In addition, the Drosophila gene encoding B-type Lamin ...
LIPID-TRANSFER PROTEINS IN PLANTS
... These assays have been used to determine the lipid transfer activity of protein extracts prepared from eukaryotic and prokaryotic cells. Among eukaryotic cells, in addition to plants, mammalian cells, yeasts, and fungi have been studied (28, 92, 108, 123, 124). These assays are important to confirm ...
... These assays have been used to determine the lipid transfer activity of protein extracts prepared from eukaryotic and prokaryotic cells. Among eukaryotic cells, in addition to plants, mammalian cells, yeasts, and fungi have been studied (28, 92, 108, 123, 124). These assays are important to confirm ...
Two dileucine motifs mediate late endosomal/lysosomal targeting of
... composition and functional protein networks in the lysosomal membrane is still fragmented [4,5]. Over the last few years proteomic approaches have addressed these questions and allowed the discovery of several novel lysosomal membrane proteins. Nevertheless a gap is still evident between the charact ...
... composition and functional protein networks in the lysosomal membrane is still fragmented [4,5]. Over the last few years proteomic approaches have addressed these questions and allowed the discovery of several novel lysosomal membrane proteins. Nevertheless a gap is still evident between the charact ...
COMBINING MOLECULAR DOCKING WITH RECEPTOR DOMAIN
... Nowadays a lot of various approaches are applied to incorporate the flexibility of receptor into the docking algorithm. As a rule, all of them are aimed at collecting a representative ensemble of protein conformations. After this is done the standard docking algorithm of a flexible ligand to rigid r ...
... Nowadays a lot of various approaches are applied to incorporate the flexibility of receptor into the docking algorithm. As a rule, all of them are aimed at collecting a representative ensemble of protein conformations. After this is done the standard docking algorithm of a flexible ligand to rigid r ...
involvement of protein tyrosine phosphorylation of human sperm in
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
... stimulating factor-1 (CSF-1) (30), interferon-γ (IFN-γ ) (35), tumor necrosis factor-α (TNF-α) (34), epidermal growth factor (EGF) (37), thymosin α1 (Tα1) (9), thymosin ß4 (Tß4) (9); and several unidentified factors (29). Some of these cytokines are also present in cervical mucus of women (38, 39), ...
INFERRING PROPERTY SELECTION PRESSURE FROM
... reduced symbol set. Again, this clustering is ad hoc, and by choosing a handful of clusters, the number of properties modeled is necessarily limited. In addition, each amino acid in a cluster is considered to be uniformly distant from the amino acids in every other cluster. Consequently, even the p ...
... reduced symbol set. Again, this clustering is ad hoc, and by choosing a handful of clusters, the number of properties modeled is necessarily limited. In addition, each amino acid in a cluster is considered to be uniformly distant from the amino acids in every other cluster. Consequently, even the p ...
Human Signaling Protein 14-3-3 Interacts With
... The initiation of primary hemostasis is mediated by interaction of the platelet glycoprotein Ib (GPIb) surface receptor and its arterial subendothelial von Willebrand factor (vWF) ligand. The intracellular signaling immediately following GPIb receptor occupancy connecting the adhesive event to plate ...
... The initiation of primary hemostasis is mediated by interaction of the platelet glycoprotein Ib (GPIb) surface receptor and its arterial subendothelial von Willebrand factor (vWF) ligand. The intracellular signaling immediately following GPIb receptor occupancy connecting the adhesive event to plate ...
161021 NGF revised Manuscript with figs
... VH domain (VHCDR3); residues Asp100-Leu100e defined by Kabat et al. 28 However, no changes were ...
... VH domain (VHCDR3); residues Asp100-Leu100e defined by Kabat et al. 28 However, no changes were ...
Thermostability of the human respiratory syncytial virus fusion
... neither sequence nor predicted structural features with the 3These authors contributed equally to this work. ...
... neither sequence nor predicted structural features with the 3These authors contributed equally to this work. ...
Calcium binding to chromaffin vesicle matrix proteins
... and Hepes were used as buffer substances at pH 6.0and 8.0, respectively. All buffers used for dialysis also contained 0.5 g of Chelex-100/200 mL, in order to remove residual Ca2+ before titration. After dialysis, Ca2+concentrations between IOd and IO-’ were determined in the samples. The Ca2+ concen ...
... and Hepes were used as buffer substances at pH 6.0and 8.0, respectively. All buffers used for dialysis also contained 0.5 g of Chelex-100/200 mL, in order to remove residual Ca2+ before titration. After dialysis, Ca2+concentrations between IOd and IO-’ were determined in the samples. The Ca2+ concen ...
Tsui, S, Dai, T, Warren, ST and Yen, P: Association of the mouse infertility factor DAZL1 with actively translating polyribosomes. Biology of Reproduction 62:1655-1660 (2000).
... domain and the DAZ repeats, but they have different Cterminal sequences due to a frame shift downstream of the DAZ repeat region. It was proposed that the DAZ genes originated from translocation of an ancestral DAZL1 gene to the Y chromosome, followed by amplification and pruning [10]. A role for DA ...
... domain and the DAZ repeats, but they have different Cterminal sequences due to a frame shift downstream of the DAZ repeat region. It was proposed that the DAZ genes originated from translocation of an ancestral DAZL1 gene to the Y chromosome, followed by amplification and pruning [10]. A role for DA ...
Journal of Applied Microbiology
... proteins with affinity for mucus constituents, where the F80 was subjected to Western blot analysis, using POD-mucin or POD-lactoferrin as a probe. As shown in Fig. 1b, two main reactive proteins with apparent molecular mass of 37 and 48 kDa were detected, indicating the affinity of F80 proteins for ...
... proteins with affinity for mucus constituents, where the F80 was subjected to Western blot analysis, using POD-mucin or POD-lactoferrin as a probe. As shown in Fig. 1b, two main reactive proteins with apparent molecular mass of 37 and 48 kDa were detected, indicating the affinity of F80 proteins for ...
nmr.evilia.190303
... The cells are grown in minimal media made with uniformly labeled 15N ammonium chloride or 13C glucose. Protein purification proceeds as without labeling ...
... The cells are grown in minimal media made with uniformly labeled 15N ammonium chloride or 13C glucose. Protein purification proceeds as without labeling ...
Characterization of Proteins Structurally Related to Human N
... preparations used to raise the antisera were not in fact homogeneous, although they were judged to be so on the basis of a single protein band after electrophoresis in polyacrylamide gel and a single schlieren boundary in the ultracentrifuge (Srivastava et al., 1974a,b). A small amount of a highly i ...
... preparations used to raise the antisera were not in fact homogeneous, although they were judged to be so on the basis of a single protein band after electrophoresis in polyacrylamide gel and a single schlieren boundary in the ultracentrifuge (Srivastava et al., 1974a,b). A small amount of a highly i ...
Challenges in characterising biopharmaceuticals for ease of
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
... characterisation during bioprocess development and product formulation. It also brought in academics with interests in these challenges, as well as those involved in developing biophysical and associated computational or data handling techniques, that may have a relevant impact in future. The meetin ...
Nuclear function for the actin-binding cytoskeletal protein
... In the last few years, it has been shown that, besides the nuclear intermedier filamentforming lamins, several cytoskeletal components are also present in the nucleus including actin, motor proteins and crosslinking proteins (Simon and Wilson 2011). However, the existence of a structure mechanically ...
... In the last few years, it has been shown that, besides the nuclear intermedier filamentforming lamins, several cytoskeletal components are also present in the nucleus including actin, motor proteins and crosslinking proteins (Simon and Wilson 2011). However, the existence of a structure mechanically ...
IvDimitrov_slides
... 1. The model developed by the k Nearest Neighbors method shows the best performance on the test set comparing to the other methods. It has a good balance between specificity and sensitivity, and the highest accuracy. kNN was used further in the study. ...
... 1. The model developed by the k Nearest Neighbors method shows the best performance on the test set comparing to the other methods. It has a good balance between specificity and sensitivity, and the highest accuracy. kNN was used further in the study. ...
Lecture 5
... 2. The aas are identified are identified according to their retention times on HPLC 3. Amounts of aas present are determined fluorescent intensities. 4. Sensitive: can detect less than 1 pmol of each amino acid. ...
... 2. The aas are identified are identified according to their retention times on HPLC 3. Amounts of aas present are determined fluorescent intensities. 4. Sensitive: can detect less than 1 pmol of each amino acid. ...
Increased Expression of Cytoskeletal, Linkage, and Extracellular
... Figure 1. Illustration of the localization of the proteins investigated. a, Isolated rat myocyte shows network-like longitudinally oriented localization of -tubulin. b, Schematic representation of localization of desmin around the Z-disks of the sarcomeres. c, Localization of vinculin (Vc) as a lin ...
... Figure 1. Illustration of the localization of the proteins investigated. a, Isolated rat myocyte shows network-like longitudinally oriented localization of -tubulin. b, Schematic representation of localization of desmin around the Z-disks of the sarcomeres. c, Localization of vinculin (Vc) as a lin ...
Week 5 - profiles, HMM
... • Hidden Markov models are statistical models that were initially developed for speech recognition. • The most popular use of HMM in molecular biology is as a ‘probabilistic profile’ of a protein family, which is called a profile HMM. • Apart from this, HMMs are also used for multiple sequence al ...
... • Hidden Markov models are statistical models that were initially developed for speech recognition. • The most popular use of HMM in molecular biology is as a ‘probabilistic profile’ of a protein family, which is called a profile HMM. • Apart from this, HMMs are also used for multiple sequence al ...
Redistribution and differential extraction of soluble proteins in
... if the soluble proteins do not bind to any cellular structures, they would appear to be preferentially localized to compartments or volumes of cytoplasm from which they are least easily extracted. Furthermore, both sources of error could operate together. These errors could also occur even when cell ...
... if the soluble proteins do not bind to any cellular structures, they would appear to be preferentially localized to compartments or volumes of cytoplasm from which they are least easily extracted. Furthermore, both sources of error could operate together. These errors could also occur even when cell ...
Nuclear Localization and Interaction with COP1 Are Required for
... subfamily of single B-box proteins. About this last subfamily, almost no information on the function of its members is available. BBX24 interacts with COP1 in the yeast two-hybrid system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn finger ...
... subfamily of single B-box proteins. About this last subfamily, almost no information on the function of its members is available. BBX24 interacts with COP1 in the yeast two-hybrid system (Holm et al., 2002) and colocalizes with it in plant cells (Indorf et al., 2007). It contains two B-box Zn finger ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.