Hydrogen exchange mass spectrometry for the analysis of protein
... FIGURE 1. Overall scheme for hydrogen exchange mass spectrometry experiments. A: Pulse labeling. After a protein has been exposed to a perturbant (chemical denaturant, heat, pH, binding, complex formation, pressure, etc.), unfolded regions (gray) become labeled with deuterium (red) during a quick pu ...
... FIGURE 1. Overall scheme for hydrogen exchange mass spectrometry experiments. A: Pulse labeling. After a protein has been exposed to a perturbant (chemical denaturant, heat, pH, binding, complex formation, pressure, etc.), unfolded regions (gray) become labeled with deuterium (red) during a quick pu ...
a review of the BLAST meeting, ï - Bacterial Locomotion and Signal
... environmental changes and act upon them. In this regard, life has solved many problems that engineers face when designing autonomous systems. Imagine designing a simple robot capable of navigating between two points. Any design would involve sensors to guide the robot, a motor to move it, and a set ...
... environmental changes and act upon them. In this regard, life has solved many problems that engineers face when designing autonomous systems. Imagine designing a simple robot capable of navigating between two points. Any design would involve sensors to guide the robot, a motor to move it, and a set ...
SpoIIQ Anchors Membrane Proteins on Both Sides of
... monochrome CoolSnapHQ digital camera (Photometrics) using Metamorph software (Universal Imaging). The lipophylic membrane dye TMA-DPH (Molecular Probes) was used at a VOLUME 283 • NUMBER 8 • FEBRUARY 22, 2008 ...
... monochrome CoolSnapHQ digital camera (Photometrics) using Metamorph software (Universal Imaging). The lipophylic membrane dye TMA-DPH (Molecular Probes) was used at a VOLUME 283 • NUMBER 8 • FEBRUARY 22, 2008 ...
“molecular” versus “colloidal”: controversies in biology and
... In 1952 he proposed the α-helix as a structural element in globular proteins, with hydrogen bonds as the most important weak association in the structures of molecules; a year later James Watson and Francis Crick suggested the double helix structure of DNA, with the two chains linked by hydrogen bon ...
... In 1952 he proposed the α-helix as a structural element in globular proteins, with hydrogen bonds as the most important weak association in the structures of molecules; a year later James Watson and Francis Crick suggested the double helix structure of DNA, with the two chains linked by hydrogen bon ...
CHAPTER FOUR Pseudomonas aeruginosa INFLUENCED PLANKTONIC AND BIOFILM POPULATIONS BASED UPON
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
... sessile multicellular communities known as biofilms (Costerton et al., 1987; 1995). The transition to surface-attached (biofilm) growth is known to result in diverse changes in gene expression, which causes the attaching cells to become phenotypically and metabolically distinct from their planktonic ...
In Depth Analysis of Protein Amino Acid Sequence and PTMs with
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
... • Peptides from the set of confident proteins are “modified” in-silico by trying all possible modifications in UNIMOD. • Speed up by de novo tags PeaksPTM: Mass spectrometry-based identification of peptides with unspecified modifications. Journal of Proteome Research 10.7 (2011) : 2930-2936 ...
Mitochondrial quality control by the ubiquitin
... proteasome. Several observations support the existence of such a system. Proteins associated with various mitochondrial compartments are ubiquitinated and subjected to proteasomal degradation, but without a mechanistic explanation this could be discounted as being pre-import regulation. If an ERAD-l ...
... proteasome. Several observations support the existence of such a system. Proteins associated with various mitochondrial compartments are ubiquitinated and subjected to proteasomal degradation, but without a mechanistic explanation this could be discounted as being pre-import regulation. If an ERAD-l ...
Biological membranes - Essays in Biochemistry
... around on their head-to-tail axis, and their lipid tails are very flexible. These different types of movements create a dynamic, fluid membrane which surrounds cells and organelles. Membrane proteins can also move laterally in the bilayer, but their rates of movement vary and are generally slower th ...
... around on their head-to-tail axis, and their lipid tails are very flexible. These different types of movements create a dynamic, fluid membrane which surrounds cells and organelles. Membrane proteins can also move laterally in the bilayer, but their rates of movement vary and are generally slower th ...
Role of protein methylation in chromatin remodeling and
... vitro was found in transcriptionally active macronuclei of Tetrahymena but not in transcriptionally inactive micronuclei (Strahl et al., 1999). In the same study methylation of yeast histones was found preferentially on H3 molecules that were also acetylated, thus providing another indication that h ...
... vitro was found in transcriptionally active macronuclei of Tetrahymena but not in transcriptionally inactive micronuclei (Strahl et al., 1999). In the same study methylation of yeast histones was found preferentially on H3 molecules that were also acetylated, thus providing another indication that h ...
Article
... does not contain exogenous hemin. Researchers have reported that RRL that works well for studies of degradation is usually poor for translation.51 Additionally, since ERAD is ATP‑dependent, the RRL will need to be supplemented with ATP and an ATP regeneration system,51 and some authors report that e ...
... does not contain exogenous hemin. Researchers have reported that RRL that works well for studies of degradation is usually poor for translation.51 Additionally, since ERAD is ATP‑dependent, the RRL will need to be supplemented with ATP and an ATP regeneration system,51 and some authors report that e ...
Protein Import, Replication, and Inheritance of a Vestigial
... derstanding of mitochondrial evolution and of the minimal set of proteins required for the biogenesis and inheritance of an endosymbiosis-derived organelle (7–9). Giardia lamblia is a unicellular protozoan parasite of the small intestine in vertebrates and a leading cause of diarrheal disease worldw ...
... derstanding of mitochondrial evolution and of the minimal set of proteins required for the biogenesis and inheritance of an endosymbiosis-derived organelle (7–9). Giardia lamblia is a unicellular protozoan parasite of the small intestine in vertebrates and a leading cause of diarrheal disease worldw ...
Protein Dynamics in the Plant Extracellular Space
... molar strength, some proteins resist solubilization due to their ionic interactions with wall components. So, harsher methodologies have to be applied with the eventual inconvenience of getting higher levels of cytoplasmic contamination [22]. Non-disruptive methods have also been used to reduce cont ...
... molar strength, some proteins resist solubilization due to their ionic interactions with wall components. So, harsher methodologies have to be applied with the eventual inconvenience of getting higher levels of cytoplasmic contamination [22]. Non-disruptive methods have also been used to reduce cont ...
Protein Supplies for Beef Cattle Diets
... nitrogen (NPN) and are generally higher in price per unit of protein. Be sure to read all feed tags checking for NPN content in range cubes, protein blocks, and liquid supplements in particular. The molasses content of liquid supplements is usually not high enough for proper NPN utilization when sup ...
... nitrogen (NPN) and are generally higher in price per unit of protein. Be sure to read all feed tags checking for NPN content in range cubes, protein blocks, and liquid supplements in particular. The molasses content of liquid supplements is usually not high enough for proper NPN utilization when sup ...
Different subcellular localisations of TRIM22 suggest species
... Most TRIM proteins, which were reported in an antiviral context (e.g. TRIM1, 5, 22, 26 and 34), possess a B30.2 domain at the C-terminus. Stremlau et al. (2005) reported that the B30.2 domain of TRIM5α is responsible for HIV-1 restriction. Song et al. (2005) investigated TRIM5α and other related TRI ...
... Most TRIM proteins, which were reported in an antiviral context (e.g. TRIM1, 5, 22, 26 and 34), possess a B30.2 domain at the C-terminus. Stremlau et al. (2005) reported that the B30.2 domain of TRIM5α is responsible for HIV-1 restriction. Song et al. (2005) investigated TRIM5α and other related TRI ...
Roles of F-box Proteins in Plant Hormone Responses
... The Arabidopsis genome encodes 11 Cullin homologs, 2 Rbx1 homologs, 21 Arabidopsis Skp1 homologs and at least 700 putative F-box proteins [16,17]. Characteristics of F-box proteins F-box proteins contain a conserved F-box domain (35− 60 amino acids) in the amino-terminus and different substrate-bind ...
... The Arabidopsis genome encodes 11 Cullin homologs, 2 Rbx1 homologs, 21 Arabidopsis Skp1 homologs and at least 700 putative F-box proteins [16,17]. Characteristics of F-box proteins F-box proteins contain a conserved F-box domain (35− 60 amino acids) in the amino-terminus and different substrate-bind ...
- Wiley Online Library
... domain from a GCN4-bZip eukaryotic transcriptional activator, a small protein capable of targeting DNA binding, as a simple model to study fluorous effect. After substitution of all leucine and valine residues by tFLeu and trifluorovaline, respectively, an increase in the melting point from 47°C to ...
... domain from a GCN4-bZip eukaryotic transcriptional activator, a small protein capable of targeting DNA binding, as a simple model to study fluorous effect. After substitution of all leucine and valine residues by tFLeu and trifluorovaline, respectively, an increase in the melting point from 47°C to ...
The structural basis of substrate translocation by the
... [33]. These two arginines were therefore proposed to lie at the substrate-binding site [33]. On the cytoplasmic side of residue Arg275, accessibility studies of single cysteine mutants showed that Cys265, Asn268, Ile269, Leu271 and Val273 of H7 line the substrate translocation pathway [34,35], and f ...
... [33]. These two arginines were therefore proposed to lie at the substrate-binding site [33]. On the cytoplasmic side of residue Arg275, accessibility studies of single cysteine mutants showed that Cys265, Asn268, Ile269, Leu271 and Val273 of H7 line the substrate translocation pathway [34,35], and f ...
Overexpression of yeast karyopherin Pse1p/Kap121p stimulates the
... A. Mitochondrial proteins used in this study are distributed according to their mesohydrophobicity values (which is an average regional hydrophobicity value determined over a 60±80 residue window) and to the hydrophobicity of the most hydrophobic 17 residue segment (< H17 >) using the MITOPROT II so ...
... A. Mitochondrial proteins used in this study are distributed according to their mesohydrophobicity values (which is an average regional hydrophobicity value determined over a 60±80 residue window) and to the hydrophobicity of the most hydrophobic 17 residue segment (< H17 >) using the MITOPROT II so ...
INPS: predicting the impact of non-synonymous variations on protein
... residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrease (two class predictors) and (iii) predict whether a mutation is stabilizing, destabilizing or not affecting the protein stability (three class predictors). Noticeably, it is also very difficult to fi ...
... residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrease (two class predictors) and (iii) predict whether a mutation is stabilizing, destabilizing or not affecting the protein stability (three class predictors). Noticeably, it is also very difficult to fi ...
Spirulina - Sun Gates Center
... Spirulina is blue - green algae high in chlorophyll, iron, carotenoids, anti-oxidants and other micronutrients. As is the case with all whole foods, Spirulina has compounds that do much more for our health than the obvious. It has been found to be effective against a series of viruses and is being s ...
... Spirulina is blue - green algae high in chlorophyll, iron, carotenoids, anti-oxidants and other micronutrients. As is the case with all whole foods, Spirulina has compounds that do much more for our health than the obvious. It has been found to be effective against a series of viruses and is being s ...
The evolution, function, structure, and expression
... oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular chaperones that prevent misfolding and irreversible aggregation of their client proteins. Most notably, the sHSPs ...
... oligomeric form with a β-sandwich that forms a hollow ball. This conservation occurs despite significant divergence in primary sequences. It is well established that sHSPs are molecular chaperones that prevent misfolding and irreversible aggregation of their client proteins. Most notably, the sHSPs ...
Preventing Shipping Fever Pneumonia in Calves
... Shipping fever pneumonia is a respiratory disease typically found in calves that causes high fever, rapid and shallow respiration, coughing and a reduced appetite. The costs of prevention and treatment may vary but can be quite considerable for cattle producers. It is believed that exposure to stres ...
... Shipping fever pneumonia is a respiratory disease typically found in calves that causes high fever, rapid and shallow respiration, coughing and a reduced appetite. The costs of prevention and treatment may vary but can be quite considerable for cattle producers. It is believed that exposure to stres ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.