Pavel Doležal
... More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mit ...
... More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mit ...
Lecture 10. Glycoproteomics
... Diverse Glycomic Data • Efforts to correlate large data sets obtained from glycomic, l i transcriptomic, t i t i genomic, i and d proteomic t i studies t di have met with several challenges. • Representation of glycan chemical structures is difficult because of their complexity and branching pattern ...
... Diverse Glycomic Data • Efforts to correlate large data sets obtained from glycomic, l i transcriptomic, t i t i genomic, i and d proteomic t i studies t di have met with several challenges. • Representation of glycan chemical structures is difficult because of their complexity and branching pattern ...
Function of Conserved Tryptophans in the Aspergillus niger
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
No Slide Title
... protein •62kD protein is Phosphoglucomutase (Glc-1-PGlc-6-P) •Transferase and phosphodiesterase found on many cells •Membrane association regulated by modification with Glc-1-P •Structure of underlying oligosaccharide not known ...
... protein •62kD protein is Phosphoglucomutase (Glc-1-PGlc-6-P) •Transferase and phosphodiesterase found on many cells •Membrane association regulated by modification with Glc-1-P •Structure of underlying oligosaccharide not known ...
Leukaemia Section t(11;14)(q23;q32) Atlas of Genetics and Cytogenetics in Oncology and Haematology
... Location 11q23 DNA/RNA 36 exons, multiple transcripts 13-15 kb. Protein 3969 amino acids; 431 kDa; contains two DNA binding motifs (a AT hook and a CXXC domain), a DNA methyl transferase motif, a bromodomain. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus, called MLL-N and M ...
... Location 11q23 DNA/RNA 36 exons, multiple transcripts 13-15 kb. Protein 3969 amino acids; 431 kDa; contains two DNA binding motifs (a AT hook and a CXXC domain), a DNA methyl transferase motif, a bromodomain. MLL is cleaved by taspase 1 into 2 proteins before entering the nucleus, called MLL-N and M ...
Targeting to the T. gondii plastid
... by the corresponding plastid genome; most plastid proteins are encoded in the nucleus, and imported post-translationally from the cytoplasm into the plastid (Keegstra and Cline, 1999). Proteins destined to reside in plastids that have two membranes, such as the chloroplasts of green plants, typicall ...
... by the corresponding plastid genome; most plastid proteins are encoded in the nucleus, and imported post-translationally from the cytoplasm into the plastid (Keegstra and Cline, 1999). Proteins destined to reside in plastids that have two membranes, such as the chloroplasts of green plants, typicall ...
binding domains demonstrated in a plant split
... or pea Toc33 (designated psToc34) and Toc159 are purified as soluble recombinant proteins from bacteria, they exist in a concentration-dependent equilibrium between the monomeric and dimeric state (Reddick et al., 2007; Yeh et al., 2007). This observation and the crystal structures available for Ara ...
... or pea Toc33 (designated psToc34) and Toc159 are purified as soluble recombinant proteins from bacteria, they exist in a concentration-dependent equilibrium between the monomeric and dimeric state (Reddick et al., 2007; Yeh et al., 2007). This observation and the crystal structures available for Ara ...
Functional Equivalence of Translation Factor eIF5B from Candida
... Phylogenetic analysis of the eIF5B of various organisms Based on the high level of sequence similarity between the eIF5Bs of various organisms, it has been argued that eIF5B could be an indicator of evolutionary divergence from archaea to humans (Lee et al., 1999). Since the cDNA sequences of eIF5B ...
... Phylogenetic analysis of the eIF5B of various organisms Based on the high level of sequence similarity between the eIF5Bs of various organisms, it has been argued that eIF5B could be an indicator of evolutionary divergence from archaea to humans (Lee et al., 1999). Since the cDNA sequences of eIF5B ...
Lecture 24
... Protein microarrays are miniaturized arrays containing small amounts of immobilized proteins, allowing the high-throughput study of a very large (100s-1000s) number of proteins. The biggest challenge in producing such arrays is the expression of proteins on such large-scale, without any loss in thei ...
... Protein microarrays are miniaturized arrays containing small amounts of immobilized proteins, allowing the high-throughput study of a very large (100s-1000s) number of proteins. The biggest challenge in producing such arrays is the expression of proteins on such large-scale, without any loss in thei ...
Atg18 function in autophagy is regulated by specific sites within its b
... Fig. 3A, wild type Atg18 and Atg18(L1) were able to complement this defect, indicating that loop 1 is not required for bulk autophagy. In contrast, a complete block of the pathway was observed when this mutation was combined with that in loop 2. The relevance of loop 2 in autophagy was also undersco ...
... Fig. 3A, wild type Atg18 and Atg18(L1) were able to complement this defect, indicating that loop 1 is not required for bulk autophagy. In contrast, a complete block of the pathway was observed when this mutation was combined with that in loop 2. The relevance of loop 2 in autophagy was also undersco ...
N-Methylated Amino Acids
... of asparagine and glutamine residues Nγ-Methylated asparagine residues are present at position 72 of the β-subunit of many phycobilins. Together with the α-subunit they form the structural building blocks of phycobilisomes. These are multimeric complexes which harvest and transfer light energy to ph ...
... of asparagine and glutamine residues Nγ-Methylated asparagine residues are present at position 72 of the β-subunit of many phycobilins. Together with the α-subunit they form the structural building blocks of phycobilisomes. These are multimeric complexes which harvest and transfer light energy to ph ...
Amino Acids
... some amino acids in vegetables as well. The body must digest the food and break it down into singular amino acids. The food protein cannot be absorbed directly, because the food sources have polypeptides with hundreds, or even thousands of amino acids joined together in peptide bonds. These must ...
... some amino acids in vegetables as well. The body must digest the food and break it down into singular amino acids. The food protein cannot be absorbed directly, because the food sources have polypeptides with hundreds, or even thousands of amino acids joined together in peptide bonds. These must ...
Expression and purification of proteins using Strep
... between the protein and the tag promotes the accessibility of the tag. The further improved Twin-Strep-tag® is a sequential arrangement of two Strep-tag®II sequences with an internal linker region (total size of 28 aa). This tag enables the same mild and rapid purification as Strep-tag®II but, in ad ...
... between the protein and the tag promotes the accessibility of the tag. The further improved Twin-Strep-tag® is a sequential arrangement of two Strep-tag®II sequences with an internal linker region (total size of 28 aa). This tag enables the same mild and rapid purification as Strep-tag®II but, in ad ...
Lectures 1 and 2
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
... Proteins can bind at the ends of N-linked and O-linked chains, and typically have binding pockets ...
PowerPoint 演示文稿
... The α helix structure is a right-handed helix with 3.6amino acid residues per turn in naturally occurring. It is the predominant structure of the polypeptide.α ...
... The α helix structure is a right-handed helix with 3.6amino acid residues per turn in naturally occurring. It is the predominant structure of the polypeptide.α ...
N1 Eukaryotic transcription factors
... • myoD also activates expression of p21waf1/cip1 expression, a small molecule inhibitor of CDKs, causing cells arrested at the G1-phase of the cell cycle which is characteristic of differentiated cells. . ...
... • myoD also activates expression of p21waf1/cip1 expression, a small molecule inhibitor of CDKs, causing cells arrested at the G1-phase of the cell cycle which is characteristic of differentiated cells. . ...
2-Oxoacid dehydrogenase multienzyme complexes
... E2 genes of the PDHC from Gram-positive organisms. Structural predictions of the proposed protein product of the E2 gene show a domain structure characteristic of the E2 component in PDHCs, and catalytically important residues, including the lysine to which the lipoic acid cofactor is covalently bou ...
... E2 genes of the PDHC from Gram-positive organisms. Structural predictions of the proposed protein product of the E2 gene show a domain structure characteristic of the E2 component in PDHCs, and catalytically important residues, including the lysine to which the lipoic acid cofactor is covalently bou ...
NMR Analysis of Protein
... changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peaks incur changes detrimental interaction such as unfolding or aggregation ...
... changes in peak position indicate binding identity of peaks that change identifies binding site on protein surface if a defined residue cluster is not observed non-specific binding if a majority of the peaks incur changes detrimental interaction such as unfolding or aggregation ...
Saito et al, 2009 - The EMBO Journal
... mammalian cells. The luminal portion of the TANGO1 protein is comprised of an SH3-like domain at its N-terminus followed by a coiled-coil domain; the cytoplasmic portion contains two coiled-coil domains and a PRD at the C-terminus. TANGO1 contains two closely spaced hydrophobic stretches: amino acid ...
... mammalian cells. The luminal portion of the TANGO1 protein is comprised of an SH3-like domain at its N-terminus followed by a coiled-coil domain; the cytoplasmic portion contains two coiled-coil domains and a PRD at the C-terminus. TANGO1 contains two closely spaced hydrophobic stretches: amino acid ...
Surface Plasmon Resonance Imaging of Transcription Factor Proteins
... the control of gene expression and the regulation of genetic information. The general structural motif that is found in the DNA binding region of many transcription regulatory proteins, the helix-turn-helix structure, can be altered to recognize different target sequences through variations in the a ...
... the control of gene expression and the regulation of genetic information. The general structural motif that is found in the DNA binding region of many transcription regulatory proteins, the helix-turn-helix structure, can be altered to recognize different target sequences through variations in the a ...
Endoplasmic Reticulum Protein Quality Control and
... nonspecific hydrophobic interactions leading to the formation of protein aggregates. Chaperones are also thought to delay the folding of proteins during synthesis. Protein synthesis is relatively slow, proceeding at a rate of ;4 amino acids s21, while the hydrophobic collapse of a protein can occur ...
... nonspecific hydrophobic interactions leading to the formation of protein aggregates. Chaperones are also thought to delay the folding of proteins during synthesis. Protein synthesis is relatively slow, proceeding at a rate of ;4 amino acids s21, while the hydrophobic collapse of a protein can occur ...
Stabilization by GroEL, a Molecular Chaperone, and a Periplasmic
... The terminal reductase of such respiration, DMSO reductase (DMSOR), is a soluble periplasmic protein consisting of a single polypeptide and containing one molecule of a molybdenum (Mo) cofactor per molecule (Satoh et al. 1987). DMSOR is synthesized as a precursor, with a molecular mass of 89,206 Da. ...
... The terminal reductase of such respiration, DMSO reductase (DMSOR), is a soluble periplasmic protein consisting of a single polypeptide and containing one molecule of a molybdenum (Mo) cofactor per molecule (Satoh et al. 1987). DMSOR is synthesized as a precursor, with a molecular mass of 89,206 Da. ...
Clean, Burn and Shape
... The Shape Nutritional Shake is vegetarian, has very few net carbs and is therefore friendly to blood sugar levels, includes all essential ...
... The Shape Nutritional Shake is vegetarian, has very few net carbs and is therefore friendly to blood sugar levels, includes all essential ...
The Distribution of Polycomb-Group Proteins During Cell Division
... 1991). It may be assumed that a better understanding of the mechanism of PcG-mediated repression in Drosophila will have broad ramifications with respect to the problem of developmental control in higher eukaryotes. However, neither a description of the molecular interactions by which PcG repression ...
... 1991). It may be assumed that a better understanding of the mechanism of PcG-mediated repression in Drosophila will have broad ramifications with respect to the problem of developmental control in higher eukaryotes. However, neither a description of the molecular interactions by which PcG repression ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.