Application of a bacterial two-hybrid system for the
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
... peak sizes of the recombinant proteins could be determined. For the monomers of FmhB, FemA and FemB, the measured molecular masses correlated well with the theoretical values derived from the amino acid sequences, and it was determined that FemA and FemB formed dimers (Fig. 3a, b; Table 3). GST-pull ...
Amino acid pool
... The cycle occurs mainly in the liver. Ammonia, which is very toxic in humans,( particularly to the central nervous system), is converted to urea , which is nontoxic, very soluble, and readily excreted by kidneys. -The concentration of ammonia and ammonium ions in the blood is normally ...
... The cycle occurs mainly in the liver. Ammonia, which is very toxic in humans,( particularly to the central nervous system), is converted to urea , which is nontoxic, very soluble, and readily excreted by kidneys. -The concentration of ammonia and ammonium ions in the blood is normally ...
MB207_10 - MB207Jan2010
... aqueous phase on one or both sides of the membrane. Protruding from one side of the bilayer: integral monotopic proteins Protruding form both sides of the bilayer: transmembrane proteins Transmembrane proteins: - cross the membrane either once (singlepass proteins) or several times (multipass protei ...
... aqueous phase on one or both sides of the membrane. Protruding from one side of the bilayer: integral monotopic proteins Protruding form both sides of the bilayer: transmembrane proteins Transmembrane proteins: - cross the membrane either once (singlepass proteins) or several times (multipass protei ...
Proteomic Analysis of the Arabidopsis Nucleolus Suggests Novel
... com) and Beven et al. (1995, 1996) showed accumulation of snoRNAs and the presence of spliceosomal snRNAs in the nucleolar cavity. Although many components of the nucleolus and other nuclear bodies have been identified, a full understanding of their functions will require far greater knowledge of th ...
... com) and Beven et al. (1995, 1996) showed accumulation of snoRNAs and the presence of spliceosomal snRNAs in the nucleolar cavity. Although many components of the nucleolus and other nuclear bodies have been identified, a full understanding of their functions will require far greater knowledge of th ...
Article
... still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as complete as those of meat, fish, milk and dairy products, which instead have all nine essential ...
... still a scarce and precious commodity, legumes were the main source of protein, especially for people who, living far from the sea, could not take advantage of fishing. The proteins of legumes are not as complete as those of meat, fish, milk and dairy products, which instead have all nine essential ...
Ion homeostasis, channels, and transporters: an update on cellular
... transport proteins that facilitate the physical translocation of ions by mechanisms that involve relatively little energetic interaction between the channel protein and the transported ion(s). Rather, extrinsic factors, such as changes in membrane potential or the binding of small regulatory molecul ...
... transport proteins that facilitate the physical translocation of ions by mechanisms that involve relatively little energetic interaction between the channel protein and the transported ion(s). Rather, extrinsic factors, such as changes in membrane potential or the binding of small regulatory molecul ...
PDF version of paper
... TEXshade, a LATEX package provides an ideal solution of displaying the key changes in DNA (4 letters: A, T, G and C) or protein (20 different amino acids, indicated by unique single letters) sequences with great control. Other programs do exist that provide a way to display sequence similarities in ...
... TEXshade, a LATEX package provides an ideal solution of displaying the key changes in DNA (4 letters: A, T, G and C) or protein (20 different amino acids, indicated by unique single letters) sequences with great control. Other programs do exist that provide a way to display sequence similarities in ...
Ch03Pt3
... one methyl shorter than Lys, but otherwise like it having a amino-R group, we can give it the one letter amino acid code “O”. Data: a. Complete acid hydrolysis followed by amino acid analysis showed L, O, F, P. V in equimolar amounts. b. The molecular weight was about 1,200. This means that there ar ...
... one methyl shorter than Lys, but otherwise like it having a amino-R group, we can give it the one letter amino acid code “O”. Data: a. Complete acid hydrolysis followed by amino acid analysis showed L, O, F, P. V in equimolar amounts. b. The molecular weight was about 1,200. This means that there ar ...
Initial characterization of ayrRABC
... sequences after translocation across the cytoplasmic membrane. Here, we identify the Staphylococcus aureus operon ayrRABC (SA0337 to SA0340) and show that once released from repression by AyrR, the protein products AyrABC together confer resistance to the SPase inhibitor arylomycin M131 by providing ...
... sequences after translocation across the cytoplasmic membrane. Here, we identify the Staphylococcus aureus operon ayrRABC (SA0337 to SA0340) and show that once released from repression by AyrR, the protein products AyrABC together confer resistance to the SPase inhibitor arylomycin M131 by providing ...
PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 Is a Plant
... PHOSPHATE TRANSPORTER1 (PHT1) genes encode phosphate (Pi) transporters that play a fundamental role in Pi acquisition and remobilization in plants. Mutation of the Arabidopsis thaliana PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) impairs Pi transport, resulting in the constitutive expression of ...
... PHOSPHATE TRANSPORTER1 (PHT1) genes encode phosphate (Pi) transporters that play a fundamental role in Pi acquisition and remobilization in plants. Mutation of the Arabidopsis thaliana PHOSPHATE TRANSPORTER TRAFFIC FACILITATOR1 (PHF1) impairs Pi transport, resulting in the constitutive expression of ...
Biological electron-transfer reactions
... complementary types of information. Spectroelectrochemistry provides a convenient means of performing potentiometric titrations while monitoring the electronic absorption spectrum of the protein [10,11]. The principle underlying spectroelectrochemical titrations is identical to that of more traditio ...
... complementary types of information. Spectroelectrochemistry provides a convenient means of performing potentiometric titrations while monitoring the electronic absorption spectrum of the protein [10,11]. The principle underlying spectroelectrochemical titrations is identical to that of more traditio ...
Translation05
... large polyprotein. Processing is carried out by two virus encoded proteases 2A pro and 3C pro. Flaviviruses- Viral precursor proteins are processed by cellular proteases. The (+) sense RNA genome is translated into a polyprotein precursor processed by viral serine protease and by host signal peptida ...
... large polyprotein. Processing is carried out by two virus encoded proteases 2A pro and 3C pro. Flaviviruses- Viral precursor proteins are processed by cellular proteases. The (+) sense RNA genome is translated into a polyprotein precursor processed by viral serine protease and by host signal peptida ...
MB207_10 - MB207Jan2010
... aqueous phase on one or both sides of the membrane. Protruding from one side of the bilayer: integral monotopic proteins Protruding form both sides of the bilayer: transmembrane proteins Transmembrane proteins: - cross the membrane either once (singlepass proteins) or several times (multipass protei ...
... aqueous phase on one or both sides of the membrane. Protruding from one side of the bilayer: integral monotopic proteins Protruding form both sides of the bilayer: transmembrane proteins Transmembrane proteins: - cross the membrane either once (singlepass proteins) or several times (multipass protei ...
Native and Artificial Reticuloplasmins Co
... inside the ER lumen, the signal peptide is removed from the nascent polypeptide and the cleaved polypeptide associates with ER-resident enzymes and molecular chaperones that help to fold it into its native conformation. Other posttranslational modifications, such as multisubunit assembly and glycosy ...
... inside the ER lumen, the signal peptide is removed from the nascent polypeptide and the cleaved polypeptide associates with ER-resident enzymes and molecular chaperones that help to fold it into its native conformation. Other posttranslational modifications, such as multisubunit assembly and glycosy ...
Troponin-I Mouse Skeletal Muscle
... with cTnI detectable in the blood for up to 5 days and cTnT for 7-10 days following MI. This allows an MI to be detected if the patient presents late. Troponin T and I are very sensitive. There is always a low level release of CK and CK-MB from skeletal muscle at a low level all the time so there is ...
... with cTnI detectable in the blood for up to 5 days and cTnT for 7-10 days following MI. This allows an MI to be detected if the patient presents late. Troponin T and I are very sensitive. There is always a low level release of CK and CK-MB from skeletal muscle at a low level all the time so there is ...
Sample pages 2 PDF
... and rotating it about the line between C1 α and C2 α by 180 degrees, translating the rotated peptide unit along the line between C1 α and C2 α by the distance between C1 α and C2 α and then rotation counterclockwise along the extension of the line C1 α to C2 α by 33 degrees as shown in Figures 2.9 a ...
... and rotating it about the line between C1 α and C2 α by 180 degrees, translating the rotated peptide unit along the line between C1 α and C2 α by the distance between C1 α and C2 α and then rotation counterclockwise along the extension of the line C1 α to C2 α by 33 degrees as shown in Figures 2.9 a ...
Module Number- 3181
... Written evidence of the ability to give the required representations of amino acids and proteins and to describe specified properties, role of proteins and the role of enzyme given with performance Criteria (a) to (d) respectively. Performance evidence of the ability to analyse amino acids which wil ...
... Written evidence of the ability to give the required representations of amino acids and proteins and to describe specified properties, role of proteins and the role of enzyme given with performance Criteria (a) to (d) respectively. Performance evidence of the ability to analyse amino acids which wil ...
Evolution of Gamete Recognition Proteins
... collection of molecules, all of which involve carbohydrates (chains of various sugar residues). In starfish, three very different types of molecules in egg jelly, named ARIS, Co-ARIS, and asterosap, are involved in AR induction, presumably by binding to sperm membrane receptors. ARIS is a polysaccha ...
... collection of molecules, all of which involve carbohydrates (chains of various sugar residues). In starfish, three very different types of molecules in egg jelly, named ARIS, Co-ARIS, and asterosap, are involved in AR induction, presumably by binding to sperm membrane receptors. ARIS is a polysaccha ...
Fibrous proteins
... the walls of large arteries, and elastic ligaments. They can be stretched to several times their normal length, but recoil to their original shape when the stretching force is relaxed. ...
... the walls of large arteries, and elastic ligaments. They can be stretched to several times their normal length, but recoil to their original shape when the stretching force is relaxed. ...
Structural biology and drug design
... Protein structure determination • Virtual screening requires a known 3D structure of the target protein • The aim of virtual HTS screening (docking) is to evaluate the fit between • a set of small molecules in a library and the drug target • To calculate interaction energies, surface contacts and st ...
... Protein structure determination • Virtual screening requires a known 3D structure of the target protein • The aim of virtual HTS screening (docking) is to evaluate the fit between • a set of small molecules in a library and the drug target • To calculate interaction energies, surface contacts and st ...
Protein translocation pathways across the inner and outer
... recently identified specific protein import machinery for small intermembrane space proteins4, but little is known about this pathway. Since mitochondria play essential roles in eukaryotic cells, knowledge regarding the protein import and biogenesis of this organelle is equally important. In this re ...
... recently identified specific protein import machinery for small intermembrane space proteins4, but little is known about this pathway. Since mitochondria play essential roles in eukaryotic cells, knowledge regarding the protein import and biogenesis of this organelle is equally important. In this re ...
The Effect of Osmotic Shock on Release of Bacterial Proteins and on
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
... called the shock fluid, is removed. This fluid contains the hydrolytic enzymes already referred to (Table I) as well as the binding proteins. It contains about 3.5 % of the cellular protein when the cells are grown under conditions that suppress the synthesis of alkaline phosphatase. T h e shocked c ...
A biofilm-forming marine bacterium producing proteins
... Pseudoalteromonas sp. D41 fermentation lead to the production of 3 EPS fractions: two soluble fractions separated according to their molecular weight and another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates wh ...
... Pseudoalteromonas sp. D41 fermentation lead to the production of 3 EPS fractions: two soluble fractions separated according to their molecular weight and another cell bound fraction, non soluble in a salt solution (36g/l). None of them is pure. EPS1 consists mainly in a majority of carbohydrates wh ...
Dephosphorylation of the Calcium Pump Coupled to Counterion
... Forms a jelly roll structure, and also contains the 40 amino acids at the N-terminal end of the protein Contains the Thr-Gly-Glu-Ser (TGES) sequence, amino acids 181-184 (main article). This sequence is highly conserved in Ptype ATPases, and plays an important role in the dephosphorylation of the E2 ...
... Forms a jelly roll structure, and also contains the 40 amino acids at the N-terminal end of the protein Contains the Thr-Gly-Glu-Ser (TGES) sequence, amino acids 181-184 (main article). This sequence is highly conserved in Ptype ATPases, and plays an important role in the dephosphorylation of the E2 ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.