Localization of growth and secretion of proteins in
... For detection of proteins secreted into the medium, the growing sandwiched colony was briefly lifted from the nutrient agar and a wetted polyvinylidenedifluoride (PVDF) membrane (Millipore) was positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the ...
... For detection of proteins secreted into the medium, the growing sandwiched colony was briefly lifted from the nutrient agar and a wetted polyvinylidenedifluoride (PVDF) membrane (Millipore) was positioned between the lower polycarbonate membrane and the agar medium. Glucoamylase was detected on the ...
Targeted Identification of Glycoproteins in Disease
... Many species of pathogenic bacteria are developing resistance to antibiotic treatments.(1) As such, there is a rising need for novel therapeutic targets. The glycosylated, or sugar-modified, proteins present on bacterial cell surfaces provide one such target.(2) Importantly, glycosylation patterns v ...
... Many species of pathogenic bacteria are developing resistance to antibiotic treatments.(1) As such, there is a rising need for novel therapeutic targets. The glycosylated, or sugar-modified, proteins present on bacterial cell surfaces provide one such target.(2) Importantly, glycosylation patterns v ...
... low-cost methods for protein purification are sorely needed, particularly at small scales where automation can accelerate the purification cycle time. Additionally, even at large-scale, low cost techniques that minimize the number of separation stages will increase yield and decrease overall process ...
Diapositiva 1
... Domain organization and evolution > Present in Hedhegoh proteins > Present in Hedgling proteins > Large extracellular protein that contains a hedge domain at its amino terminus plus many additional > domains such as VWA domains and numerous cadherin repeats, but lacks a Hog domain. > Found in sponge ...
... Domain organization and evolution > Present in Hedhegoh proteins > Present in Hedgling proteins > Large extracellular protein that contains a hedge domain at its amino terminus plus many additional > domains such as VWA domains and numerous cadherin repeats, but lacks a Hog domain. > Found in sponge ...
Protein Synthesis and the Stress Response
... several tRNA modification enzymes affect survival of E. coli in a milder oxidative stress condition (0.5 mM H2O2) [56]. Despite these reports, it is not clear how tRNA modifications improve survival to oxidative stress. It has been suggested that tRNA modifications increase the efficiency of transla ...
... several tRNA modification enzymes affect survival of E. coli in a milder oxidative stress condition (0.5 mM H2O2) [56]. Despite these reports, it is not clear how tRNA modifications improve survival to oxidative stress. It has been suggested that tRNA modifications increase the efficiency of transla ...
The protein import apparatus of chloroplasts
... translation product of the cDNA clone for the 30 kDa protein from pea and spinach occurs into a proteascinsensitive localization in the envelope memhranes (Fliigge et a!. 1989, Willey et a!. 1991). (3) Thc phosphate translocator activity and the protein import efficiency is largely diminished by inc ...
... translation product of the cDNA clone for the 30 kDa protein from pea and spinach occurs into a proteascinsensitive localization in the envelope memhranes (Fliigge et a!. 1989, Willey et a!. 1991). (3) Thc phosphate translocator activity and the protein import efficiency is largely diminished by inc ...
BICH/GENE 431 KNOWLEDGE OBJECTIVES Chapter 13 – RNA
... - sex determination in Drosophila (details later) Possible mechanisms for regulation - steric hindrance when intron is too small: both U1 snRNP and U2 snRNP cannot bind at same time to same intron; leads to mutually exclusive exons, like for troponin T - combination of GU-AG and AT-AC splice sites i ...
... - sex determination in Drosophila (details later) Possible mechanisms for regulation - steric hindrance when intron is too small: both U1 snRNP and U2 snRNP cannot bind at same time to same intron; leads to mutually exclusive exons, like for troponin T - combination of GU-AG and AT-AC splice sites i ...
Deciphering the genetic code Dr. Syndey Brenner estabilished mRNA
... known as the cap-binding protein (CBP or eIF4F)---CBP contains a number of small subunits, including eIF4E Several other eIFs join, and the mRNA then binds to the eIFs-MettRNAiMet-40S ribosomal complex In this reaction, hydrolysis of ATP is required because a helicase is needed to unwind the hai ...
... known as the cap-binding protein (CBP or eIF4F)---CBP contains a number of small subunits, including eIF4E Several other eIFs join, and the mRNA then binds to the eIFs-MettRNAiMet-40S ribosomal complex In this reaction, hydrolysis of ATP is required because a helicase is needed to unwind the hai ...
Leishmania Flagellum
... the characterization of individual elements. Bin/Amphiphysin/Rvs (BAR) domains are 200-amino-acid modular elements found in many eukaryotic multi-domain proteins [14]. Although their amino acid sequence may be poorly conserved and therefore difficult to identify, their structure is well conserved th ...
... the characterization of individual elements. Bin/Amphiphysin/Rvs (BAR) domains are 200-amino-acid modular elements found in many eukaryotic multi-domain proteins [14]. Although their amino acid sequence may be poorly conserved and therefore difficult to identify, their structure is well conserved th ...
PowerPoint Template
... complementary binding region on the downstrem effector protein, the Raf kinase. In addition to the Raf kinase, other downstream effectors can interact with the Ras effector domain as well, albeit with different affinities. Importantly, the availability of the binding surface for interaction with t ...
... complementary binding region on the downstrem effector protein, the Raf kinase. In addition to the Raf kinase, other downstream effectors can interact with the Ras effector domain as well, albeit with different affinities. Importantly, the availability of the binding surface for interaction with t ...
Mitochondrial protein import: from transport pathways to an
... provided a huge number of uncharacterized mitochondrial proteins [31–34], which can be studied using the available collections of mutant yeast strains. Given that mitochondrial protein import is essential for cell viability [3], it is not surprising that functional screens for import components have ...
... provided a huge number of uncharacterized mitochondrial proteins [31–34], which can be studied using the available collections of mutant yeast strains. Given that mitochondrial protein import is essential for cell viability [3], it is not surprising that functional screens for import components have ...
High Resolution Structure of the Neisseria gonorrhoeae Type IV
... aeruginosa, and a truncated structure lacking the hydrophobic N-terminus was determined for Vibrio cholerae 2, 3. In a recently published article in Molecular Cell, this group report the 2.3 Å structure of N. gonorrhoeae (GC) pilin along with a “pseudo-atomic” structure of the pilus filament, solved ...
... aeruginosa, and a truncated structure lacking the hydrophobic N-terminus was determined for Vibrio cholerae 2, 3. In a recently published article in Molecular Cell, this group report the 2.3 Å structure of N. gonorrhoeae (GC) pilin along with a “pseudo-atomic” structure of the pilus filament, solved ...
PowerPoint 演示文稿
... Helix-turn-helix domain Structure: a 60aa homeodomain encoded by the homeobox. Found in: ...
... Helix-turn-helix domain Structure: a 60aa homeodomain encoded by the homeobox. Found in: ...
The YidC/Oxa1/Alb3 protein family Saller, Manfred J.
... TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. This region possibly assists in substrate protein folding (Sven et al., 2008). The core domain of the YidC homologs of Euryarchaeota (Pohlschroder et al., 2005; Zhang et al., 2009) seems to comprise only three TMSs h ...
... TMS2 and TMS3 that is conserved within cyanobacteria and is essential for activity. This region possibly assists in substrate protein folding (Sven et al., 2008). The core domain of the YidC homologs of Euryarchaeota (Pohlschroder et al., 2005; Zhang et al., 2009) seems to comprise only three TMSs h ...
Lac
... milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found in milk of species which transport high levels of immunoglobulins during c ...
... milk of many species. The function of ß-LG is unknown; it may be a fatty acid or lipid binding protein. It does have sequence similarities with retinol-binding proteins, but this may not be its function. Generally it is found in milk of species which transport high levels of immunoglobulins during c ...
How can biochemical reactions within cells differ from those in test
... By contrast, the free energy profile in red in Fig. 3 has been shifted upward because all three states of the system interact with the background in a repulsive (free energy raising) fashion. Nonspecific intermolecular repulsion can be due to volume exclusion (steric repulsion) or electrostatic effe ...
... By contrast, the free energy profile in red in Fig. 3 has been shifted upward because all three states of the system interact with the background in a repulsive (free energy raising) fashion. Nonspecific intermolecular repulsion can be due to volume exclusion (steric repulsion) or electrostatic effe ...
Contribution of molecular chaperones to protein folding in the
... The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assembly of proteins has come from in v ...
... The process by which a linear polypeptide attains its unique, functionally active, three-dimensional structure from the astronomical number of possible conformations has perplexed researchers for many years [1]. Most of our knowledge concerning the folding and assembly of proteins has come from in v ...
Do asparagine-linked carbohydrate chains in glycoproteins have a
... occur ifglycosylation is disadvantageous for the stability or the function of the protein. Evidence for this has been presented by Sinohara and Maruyama (24), who observed that Asn-X-Ser/Thr sequences occur less frequently than random in extracellular proteins from eukaryotes. Possible functional ro ...
... occur ifglycosylation is disadvantageous for the stability or the function of the protein. Evidence for this has been presented by Sinohara and Maruyama (24), who observed that Asn-X-Ser/Thr sequences occur less frequently than random in extracellular proteins from eukaryotes. Possible functional ro ...
molecular biology
... are processed where certain nucleotides or amino acids that are non-coding or non-essential for function or structure are selectively removed to yield active molecules. Processing of RNAs and pre-proproteins yield biologically active RNA and protein molecules that are devoid of ‘introns’ as in RNA, ...
... are processed where certain nucleotides or amino acids that are non-coding or non-essential for function or structure are selectively removed to yield active molecules. Processing of RNAs and pre-proproteins yield biologically active RNA and protein molecules that are devoid of ‘introns’ as in RNA, ...
Protein import into the nucleus - Universitätsklinikum des Saarlandes
... Ran-GTP binding nucleoporin RanBP2/Nup358 was also localized to this region [51] and was shown to be a major Ran binding component of rat liver nuclear envelopes [52,62]. Regulation by specific GAPs and GEFs is required for proper function of Ran. So far only one GAP and only one GEF have been ident ...
... Ran-GTP binding nucleoporin RanBP2/Nup358 was also localized to this region [51] and was shown to be a major Ran binding component of rat liver nuclear envelopes [52,62]. Regulation by specific GAPs and GEFs is required for proper function of Ran. So far only one GAP and only one GEF have been ident ...
Pavel Doležal
... More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mit ...
... More recent studies of evolution and cell biology of “amitochondriate” protists, however, challenged their amitochondriate status, at least in the case of those organisms possessing double-membrane bound organelles such as hydrogen-producing hydrogenosomes and newly characterized organelles, the mit ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.