
Detection of RNA-protein complex in vaccinia virus core in vitro
... These proteins seem to be similar to those released d u r i n g in vitro t r a n s c r i p t i o n , p r o m p t i n g us to verify w h e t h e r cores recovered from A T P p r e - i n c u b a t i o n were ...
... These proteins seem to be similar to those released d u r i n g in vitro t r a n s c r i p t i o n , p r o m p t i n g us to verify w h e t h e r cores recovered from A T P p r e - i n c u b a t i o n were ...
Preventing Shipping Fever Pneumonia in Calves
... Shipping fever pneumonia is a respiratory disease typically found in calves that causes high fever, rapid and shallow respiration, coughing and a reduced appetite. The costs of prevention and treatment may vary but can be quite considerable for cattle producers. It is believed that exposure to stres ...
... Shipping fever pneumonia is a respiratory disease typically found in calves that causes high fever, rapid and shallow respiration, coughing and a reduced appetite. The costs of prevention and treatment may vary but can be quite considerable for cattle producers. It is believed that exposure to stres ...
Overcoming stalled translation in human mitochondria
... the final coding triplet in mitochondrial transcripts MTCO1 and MTND6 respectively, this was not an unreasonable interpretation of the human mitochondrial genome (Anderson et al., 1981). More recent investigations in whole cells have shown that physiologically neither of these are stop codons. Althou ...
... the final coding triplet in mitochondrial transcripts MTCO1 and MTND6 respectively, this was not an unreasonable interpretation of the human mitochondrial genome (Anderson et al., 1981). More recent investigations in whole cells have shown that physiologically neither of these are stop codons. Althou ...
Optimizing bacterial expression and purifica- Biomedical laboratory science,
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
... Despite the discovery of deoxyribonucleic acid (DNA) in the early 1950’s and the subsequent discovery of the structure of DNA by the 1960’s, it was only during the 1970’s, when a new methodology called recombinant DNA technology or genetic engineering was developed. This new methodology made it poss ...
The cDNA sequence and polymorphism of horse tranferrin
... transferrin was determined and used to identify sequence polymorphisms which distinguish some of the many variants of horse transferrin. A horse liver cDNA library was constructed, and was screened using the human transferrin cDNA as a probe. The clones isolated gave 1800 bp of sequence. The remaind ...
... transferrin was determined and used to identify sequence polymorphisms which distinguish some of the many variants of horse transferrin. A horse liver cDNA library was constructed, and was screened using the human transferrin cDNA as a probe. The clones isolated gave 1800 bp of sequence. The remaind ...
GPCR–G fusion proteins
... possesses the properties of a constitutively active GPCR. These differences can be explained by the lower GDPaffinity of GsaL compared to the GDP-affinity of GsaS, i.e. GsaL is more often GDP-free than GsaS and, therefore, more often available to stabilize the active R* state of the GPCR GPCR–Ga cou ...
... possesses the properties of a constitutively active GPCR. These differences can be explained by the lower GDPaffinity of GsaL compared to the GDP-affinity of GsaS, i.e. GsaL is more often GDP-free than GsaS and, therefore, more often available to stabilize the active R* state of the GPCR GPCR–Ga cou ...
18_2014_1558_MOESM8_ESM
... represents protein relative abundance. Thresholds for decreased and increased abundance were set at <0.83 and >1.2, respectively. The P-value was calculated using Stouffer’s z-test, which enables P-values from several protein ratios belonging to the same analysis group to be combined, generating a s ...
... represents protein relative abundance. Thresholds for decreased and increased abundance were set at <0.83 and >1.2, respectively. The P-value was calculated using Stouffer’s z-test, which enables P-values from several protein ratios belonging to the same analysis group to be combined, generating a s ...
TRYPSIN / LYS
... arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chym ...
... arginine residues. Unmodified trypsin is subject to autolysis, generating fragments that can interfere with protein sequencing, HPLC or mass spectrometry analysis of the peptides. In addition, autolysis can result in the generation of pseudotrypsin, which has been shown to exhibit an additional chym ...
Analysis of High Accuracy, Quantitative Proteomics Data in the
... Bottom-up proteomics consists of the MS analysis of enzymatically digested proteomes. During the last few years, measurements have increasingly been performed in a high resolution, quantitative format (1–3). Each proteomic experiment typically generates large amounts of raw MS and MS/MS data, which ...
... Bottom-up proteomics consists of the MS analysis of enzymatically digested proteomes. During the last few years, measurements have increasingly been performed in a high resolution, quantitative format (1–3). Each proteomic experiment typically generates large amounts of raw MS and MS/MS data, which ...
mic.sgmjournals.org
... Methods for spot cutting and protein digestion with trypsin were reported previously (Pieper et al., 2008). Peptide digests were analysed using a MALDI-TOFTOF mass spectrometer (4700 Proteomics Analyser, Applied Biosystems) and a nano-electrospray LC-MS/MS system (LTQ ion trap mass spectrometer, The ...
... Methods for spot cutting and protein digestion with trypsin were reported previously (Pieper et al., 2008). Peptide digests were analysed using a MALDI-TOFTOF mass spectrometer (4700 Proteomics Analyser, Applied Biosystems) and a nano-electrospray LC-MS/MS system (LTQ ion trap mass spectrometer, The ...
9 Production of Proteins from Cloned Genes
... These enzymes were originally purified from a diverse range of organisms, but now nearly all of them have been cloned and expressed in Escherichia coli, and are purified from there. Proteins also have numerous applications, many of which produce goods with which we are all familiar. Enzymes, in part ...
... These enzymes were originally purified from a diverse range of organisms, but now nearly all of them have been cloned and expressed in Escherichia coli, and are purified from there. Proteins also have numerous applications, many of which produce goods with which we are all familiar. Enzymes, in part ...
FoldSynth: Interactive 2D/3D Visualisation Platform for Molecular
... backbone. Balls can be deformed into ellipsoids that show the current velocity of each particle. Isosurface view: Shows each particle as a metaball [Bli82], with fast CPU and GPU implementations that allow realtime interactive use and blending into dynamic isosurfaces [LB06]. We use the following fa ...
... backbone. Balls can be deformed into ellipsoids that show the current velocity of each particle. Isosurface view: Shows each particle as a metaball [Bli82], with fast CPU and GPU implementations that allow realtime interactive use and blending into dynamic isosurfaces [LB06]. We use the following fa ...
Gene Section IGF1 (Insulin-Like Growth Factor 1 (Somatomedin C))
... results in increased cellular proliferation and antiapoptotic effects; promoting a favorable environment for tumor growth. In General, high levels of IGF-1 are found in solid tumors (particularly breast and prostate). Certain SNP's and IGF-1 haplotypes have been associated with increased risk of col ...
... results in increased cellular proliferation and antiapoptotic effects; promoting a favorable environment for tumor growth. In General, high levels of IGF-1 are found in solid tumors (particularly breast and prostate). Certain SNP's and IGF-1 haplotypes have been associated with increased risk of col ...
Teaching Notes
... Q. What is the function of tRNA? A. tRNA acts as a translator in the central dogma of biology – it recognizes codons in mRNA and brings in specific and corresponding amino acids to the ribosome for protein synthesis. Q. How many bases are in anti-codon region of the model A. 3 bases Q. Name of the a ...
... Q. What is the function of tRNA? A. tRNA acts as a translator in the central dogma of biology – it recognizes codons in mRNA and brings in specific and corresponding amino acids to the ribosome for protein synthesis. Q. How many bases are in anti-codon region of the model A. 3 bases Q. Name of the a ...
Chapter 7: The Control of Microbial Growth
... were examined. FINDINGS: Onset of behavioural symptoms was associated, by the parents, with measles, mumps, and rubella vaccination in eight of the 12 children, with measles infection in one child, and otitis media in another. All 12 children had intestinal abnormalities, ranging from lymphoid nodul ...
... were examined. FINDINGS: Onset of behavioural symptoms was associated, by the parents, with measles, mumps, and rubella vaccination in eight of the 12 children, with measles infection in one child, and otitis media in another. All 12 children had intestinal abnormalities, ranging from lymphoid nodul ...
Signatures of nitrogen limitation in the elemental composition of the
... mass. Therefore, if evolutionary pressures for strategic elemental investment have acted to conserve N in the catabolic apparatus, the strongest biases towards low-N monomers are expected in the catabolic machinery. We explored this hypothesis by comparing different classes of enzymes involved in th ...
... mass. Therefore, if evolutionary pressures for strategic elemental investment have acted to conserve N in the catabolic apparatus, the strongest biases towards low-N monomers are expected in the catabolic machinery. We explored this hypothesis by comparing different classes of enzymes involved in th ...
the versatile bacterial type iv secretion systems
... Mpf complex to drive DNA transfer, until recently it was not known whether the CP physically interacts with the Mpf structure. Now, two studies have reported that CPs form stable interactions with homologues of the A. tumefaciens VirB10 protein35,36. The VirB10-type proteins are bitopic inner-membra ...
... Mpf complex to drive DNA transfer, until recently it was not known whether the CP physically interacts with the Mpf structure. Now, two studies have reported that CPs form stable interactions with homologues of the A. tumefaciens VirB10 protein35,36. The VirB10-type proteins are bitopic inner-membra ...
figure 1 - Proceedings of the Royal Society B
... mass. Therefore, if evolutionary pressures for strategic elemental investment have acted to conserve N in the catabolic apparatus, the strongest biases towards low-N monomers are expected in the catabolic machinery. We explored this hypothesis by comparing different classes of enzymes involved in th ...
... mass. Therefore, if evolutionary pressures for strategic elemental investment have acted to conserve N in the catabolic apparatus, the strongest biases towards low-N monomers are expected in the catabolic machinery. We explored this hypothesis by comparing different classes of enzymes involved in th ...
Clathrinmediated transport: assembly required
... selection function; and an outer clathrin coat with the ability to polymerize and bind adaptors and other regulatory factors, which allows the concentration of associated proteins into regular protein networks. Schematic representation of clathrin (B) and AP2 (C). (D) A 21 Å resolution map of clathr ...
... selection function; and an outer clathrin coat with the ability to polymerize and bind adaptors and other regulatory factors, which allows the concentration of associated proteins into regular protein networks. Schematic representation of clathrin (B) and AP2 (C). (D) A 21 Å resolution map of clathr ...
Lipid–protein interactions probed by electron crystallography
... extended conformation while most lipids adopt contorted structures induced by shallow hydrophobic grooves displayed by the AQP0 hydrophobic belt. In these cases, the lipid acyl chains bend and buckle to fit into irregularities on the protein. Similarly, most PC head groups are found with highly bent ...
... extended conformation while most lipids adopt contorted structures induced by shallow hydrophobic grooves displayed by the AQP0 hydrophobic belt. In these cases, the lipid acyl chains bend and buckle to fit into irregularities on the protein. Similarly, most PC head groups are found with highly bent ...
A Distinct and Parallel Pathway for the Nuclear Import of an mRNA
... Sequence analysis revealed a super-family of karyopherin b homologues with members in yeast and higher eukaryotes, referred to here as b karyopherins. Several members of this family were shown to interact with Ran, and one member, human Crm1, was identified via its interaction with a repeat-contain ...
... Sequence analysis revealed a super-family of karyopherin b homologues with members in yeast and higher eukaryotes, referred to here as b karyopherins. Several members of this family were shown to interact with Ran, and one member, human Crm1, was identified via its interaction with a repeat-contain ...
npgrj_nchembio_91 405..407 - The Scripps Research Institute
... the SE probe designates it as a highly versatile electrophile for ABPP, and potentially for related chemical biology endeavors such as ligand-guided protein surface labeling14, which aims to convert reversible ligands into covalent probes by proximity-induced reactivity with nucleophilic amino acids ...
... the SE probe designates it as a highly versatile electrophile for ABPP, and potentially for related chemical biology endeavors such as ligand-guided protein surface labeling14, which aims to convert reversible ligands into covalent probes by proximity-induced reactivity with nucleophilic amino acids ...
- CSHL Institutional Repository
... somes form 1:1 complexes whereas bacteri required for the biogenesis of only a subset of n proteins, is present at !1 molecule/100 ribos bly, trigger factor and SRP bind to the same r exit of the ribosomal tunnel (1, 2). The cur trigger factor and SRP can bind simultaneous some (1); however, it was ...
... somes form 1:1 complexes whereas bacteri required for the biogenesis of only a subset of n proteins, is present at !1 molecule/100 ribos bly, trigger factor and SRP bind to the same r exit of the ribosomal tunnel (1, 2). The cur trigger factor and SRP can bind simultaneous some (1); however, it was ...
clinical toxicology unit
... diagnosis. All samples should be sent in plain tube (yellow cap). Samples can be taken at random times and fasting is not required. ...
... diagnosis. All samples should be sent in plain tube (yellow cap). Samples can be taken at random times and fasting is not required. ...
Separation of nuclear protein complexes by blue native
... solubilized in the presence of a detergent and 750 mM 6-AcA without the effect on their integrity [3]. However, we detected differences in the solubility and stability of several nuclear complexes in 750 mM 6-AcA (not shown). It was not unexpected since differences in solubility of individual nuclea ...
... solubilized in the presence of a detergent and 750 mM 6-AcA without the effect on their integrity [3]. However, we detected differences in the solubility and stability of several nuclear complexes in 750 mM 6-AcA (not shown). It was not unexpected since differences in solubility of individual nuclea ...
Intrinsically disordered proteins

An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.