Microsoft Word
... specificity eIF4E from Schistosoma mansoni in the complexes with the MMG-cap analogues, m7GpppG and m7GpppA [18], showed a similar binding mode as for the single specificity eIF4Es. The only difference seems to be in the conformation of E90 carboxylate of S. mansoni eIF4E that is rotated by ~80 in ...
... specificity eIF4E from Schistosoma mansoni in the complexes with the MMG-cap analogues, m7GpppG and m7GpppA [18], showed a similar binding mode as for the single specificity eIF4Es. The only difference seems to be in the conformation of E90 carboxylate of S. mansoni eIF4E that is rotated by ~80 in ...
Tracing the Archaeal Origins of Eukaryotic Membrane
... et al. 2014). While these observations suggest that this mechanism contributed significantly to early eukaryotic evolution, the deepest origins of the MTS remain unclear. This lack of insight may be due to the fact that, in contrast to proteins derived from endosymbiotic organelles (i.e., mitochondr ...
... et al. 2014). While these observations suggest that this mechanism contributed significantly to early eukaryotic evolution, the deepest origins of the MTS remain unclear. This lack of insight may be due to the fact that, in contrast to proteins derived from endosymbiotic organelles (i.e., mitochondr ...
Hemoglobin
... HEMOGLOBIN As deoxygenated hemoglobin becomes oxygenated, significant structural changes take place the proximal hisitidine and its helix shift one heterodimer rotates and slides relative to the other existing noncovalent bonds are broken and replaced by new ...
... HEMOGLOBIN As deoxygenated hemoglobin becomes oxygenated, significant structural changes take place the proximal hisitidine and its helix shift one heterodimer rotates and slides relative to the other existing noncovalent bonds are broken and replaced by new ...
Arginine-glycine-aspartic acid motif incorporated within turnip yellow
... A tripeptide motif used by Integrins as an attachment point. RGD motifs are found within viral proteins and facilitate cellular adhesion. Integrin (protein receptor) Integrin is a ligand used by some cells and viruses for adhesion and cell signaling. Stem cells up-regulate Integrin productio ...
... A tripeptide motif used by Integrins as an attachment point. RGD motifs are found within viral proteins and facilitate cellular adhesion. Integrin (protein receptor) Integrin is a ligand used by some cells and viruses for adhesion and cell signaling. Stem cells up-regulate Integrin productio ...
The Plant Journal
... by Kang et al. (2001) suggested that ADL1 is associated with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference ...
... by Kang et al. (2001) suggested that ADL1 is associated with the cell plate. ADL2 was localized to the plastids (Kang et al., 1998). ADL6 was shown to associate with the Golgi apparatus (Jin et al., 2001). The cellular compartment to which ADL3 associates is currently unknown. Apart from difference ...
Effects of phosphatidylethanolamine glycation on lipid–protein
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
... glycation by forming adducts with PE. Moreover, these authors found these complexes in human red blood cells, suggesting that this compound may act as a lipid glycation inhibitor in vivo, and demonstrated that supplementation of the diet of diabetic rats with pyridoxal 5 -phosphate reduces the leve ...
PolyWAX LP ERLIC instructions
... ERLIC of Amino Acids: PolyWAX LP™ columns with pore diameter of 100 Å are best for this. The limiting factor in isocratic separation of amino acids is the prolonged retention of acidic amino acids, especially aspartic acid. One can separate 16-17 amino acids isocratically with 10 mM TEAP, pH 2.0, wi ...
... ERLIC of Amino Acids: PolyWAX LP™ columns with pore diameter of 100 Å are best for this. The limiting factor in isocratic separation of amino acids is the prolonged retention of acidic amino acids, especially aspartic acid. One can separate 16-17 amino acids isocratically with 10 mM TEAP, pH 2.0, wi ...
Predicting RNA Secondary Structures
... Predicting RNA Secondary Structures It is possible to predict the secondary structure of an RNA sequence The accuracy is ~70% Predictions are easier and more accurate on small molecules (fewer than 500 nucleotides) ...
... Predicting RNA Secondary Structures It is possible to predict the secondary structure of an RNA sequence The accuracy is ~70% Predictions are easier and more accurate on small molecules (fewer than 500 nucleotides) ...
Storage globulins pass through the Golgi apparatus and
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
... storage vacuoles (PSVs). Multiple mechanisms have been proposed to be responsible for transporting storage proteins to PSVs in developing seeds. In this study, a specific antibody was raised against the mung bean (Vigna radiata) seed storage protein 8S globulin and its deposition was followed via im ...
Regulation of Photochemical Energy Transfer Accompanied
... proteins were detected using anti-pThr antibody, and phosphorylated D1/D2 proteins were quantified by densitometric analysis. As shown in Figure 3a, compared with samples under normal conditions (L25), the relative phosphorylation levels of D1/D2 proteins increased under the 40 °C treatment in light ...
... proteins were detected using anti-pThr antibody, and phosphorylated D1/D2 proteins were quantified by densitometric analysis. As shown in Figure 3a, compared with samples under normal conditions (L25), the relative phosphorylation levels of D1/D2 proteins increased under the 40 °C treatment in light ...
Keep Your Fingers Off My DNA: Protein–Protein
... (classic) zinc finger of FOG1 and TACC3, Simpson et al. [23] combined NMR and alanine mutagenesis to pinpoint critical amino acid involved in the interaction. Interestingly, they demonstrated that amino acids in the a-helix of FOG1 formed the binding surface for the interaction and that residues in ...
... (classic) zinc finger of FOG1 and TACC3, Simpson et al. [23] combined NMR and alanine mutagenesis to pinpoint critical amino acid involved in the interaction. Interestingly, they demonstrated that amino acids in the a-helix of FOG1 formed the binding surface for the interaction and that residues in ...
Facing extremes: archaeal surface-layer (glyco)proteins
... glycosylation, suggesting that motifs apart from the consensus Asn-Xaa-Ser/Thr sequence are recognized by the haloarchaeal glycosylation machinery (Zeitler et al., 1998). Finally, several studies addressing S-layer glycoprotein glycosylation suggest that in archaea, protein glycosylation occurs on t ...
... glycosylation, suggesting that motifs apart from the consensus Asn-Xaa-Ser/Thr sequence are recognized by the haloarchaeal glycosylation machinery (Zeitler et al., 1998). Finally, several studies addressing S-layer glycoprotein glycosylation suggest that in archaea, protein glycosylation occurs on t ...
ATP-binding-cassette (ABC) transport systems: Functional and
... Fig. 3. Sequence alignment of ABC domains from bacterial and eukaryotic ABC transporters. Sequences were retrieved from the SWISSPROT database and aligned using the DNASIS software package (Hitachi). The proteins considered are: MDR1NBF1, P-glycoprotein (human, N-terminal domain); MDR1NBF2, P-glycop ...
... Fig. 3. Sequence alignment of ABC domains from bacterial and eukaryotic ABC transporters. Sequences were retrieved from the SWISSPROT database and aligned using the DNASIS software package (Hitachi). The proteins considered are: MDR1NBF1, P-glycoprotein (human, N-terminal domain); MDR1NBF2, P-glycop ...
Ribosome-tethered molecular chaperones
... Around 40 amino acids of the nascent chain are protected from the cytosol by the ribosome exit tunnel [4]. Recent evidence indicates that after the chain leaves the tunnel, molecular chaperones bind, preventing aggregation. Both prokaryotic and eukaryotic chaperones have evolved to associate specifi ...
... Around 40 amino acids of the nascent chain are protected from the cytosol by the ribosome exit tunnel [4]. Recent evidence indicates that after the chain leaves the tunnel, molecular chaperones bind, preventing aggregation. Both prokaryotic and eukaryotic chaperones have evolved to associate specifi ...
... microscopy, proteomics, enzymatic activity, and serological reactivity. Secretory vesicles in C. neoformans appear to be correlated with exosome-like compartments derived from multivesicular bodies. Extracellular vesicles manifested various sizes and morphologies, including electron-lucid membrane b ...
Systematic Characterisation of Cellular Localisation and
... DRiPs would enable MHC class I molecules to monitor protein synthesis rates rather than protein concentrations, and offer the possibility of rapid detection of virus-infected cells. One implication of the DRiP hypothesis is that the correlation between protein concentration and the probability that ...
... DRiPs would enable MHC class I molecules to monitor protein synthesis rates rather than protein concentrations, and offer the possibility of rapid detection of virus-infected cells. One implication of the DRiP hypothesis is that the correlation between protein concentration and the probability that ...
... These supernatants contained vesicles and were concentrated by approximately 20-fold using an Amicon ultrafiltration system (cutoff of 100 kDa). The concentrate was again centrifuged at 4,000 and 15,000 ⫻ g (15 min, 4°C) and then at 100,000 ⫻ g for 1 h at 4°C. The supernatants were discarded, and pe ...
Identification and characterization of novel interaction partners of
... The Drosophila member of the IAP family, DIAP1 is necessary for cell survival and thus for the early embryo. Zygotic null-mutants of DIAP1 (thread 109) exhibit massive cell death during gastrulation. DIAP1 interacts both with caspases and with the proapoptotic proteins Reaper Hid and Grim (RHG). Str ...
... The Drosophila member of the IAP family, DIAP1 is necessary for cell survival and thus for the early embryo. Zygotic null-mutants of DIAP1 (thread 109) exhibit massive cell death during gastrulation. DIAP1 interacts both with caspases and with the proapoptotic proteins Reaper Hid and Grim (RHG). Str ...
Scaffold nucleoporins Nup188 and Nup192 share
... fragments of Nup188, determined the structures of these fragments, and then combined these to produce the likely structure of the full-length protein. They found that Nup188 has a structure that consists of stacked helices and is more flexible than other nups. Moreover, its structure was very simila ...
... fragments of Nup188, determined the structures of these fragments, and then combined these to produce the likely structure of the full-length protein. They found that Nup188 has a structure that consists of stacked helices and is more flexible than other nups. Moreover, its structure was very simila ...
The Proteomics Big Challenge for Biomarkers and New Drug
... deregulate these pathways, leading to disease conditions. A detailed knowledge of the pathways active inside the cell and of how they are deranged in a particular pathology is fundamental for drug discovery as it allows the identification of new drug targets. Functional proteomics focuses on the gen ...
... deregulate these pathways, leading to disease conditions. A detailed knowledge of the pathways active inside the cell and of how they are deranged in a particular pathology is fundamental for drug discovery as it allows the identification of new drug targets. Functional proteomics focuses on the gen ...
SMOOTH MUSCLE
... and A bands, & surround them. • They are surrounded by the sarcoplasm & are NOT connected to the outside of the cell. • At their both ends they show dilated ends called as Terminal cisterns or sacs. • They contain a protein called as Calsequestrin, which binds and holds CALCIUM. ...
... and A bands, & surround them. • They are surrounded by the sarcoplasm & are NOT connected to the outside of the cell. • At their both ends they show dilated ends called as Terminal cisterns or sacs. • They contain a protein called as Calsequestrin, which binds and holds CALCIUM. ...
The Origin of Life on Earth by Leslie E. Orgel Growing evidence
... the amino acids needed for the construction of proteins - and for life itself - would have been abundant on the primitive planet. At the time, investigators were not yet paying much attention to the origin of nucleic acids- they were most interested in explaining how proteins appeared on the earth. ...
... the amino acids needed for the construction of proteins - and for life itself - would have been abundant on the primitive planet. At the time, investigators were not yet paying much attention to the origin of nucleic acids- they were most interested in explaining how proteins appeared on the earth. ...
The Bacterial Phosphotransferase System: Structure, Function
... In 1964, the three recognized activities of the PTS were presumed to correspond merely to three proteins. We now recognize dozens of PTS proteins in E. coli as well as hundreds of PTS proteins in other bacteria. Numerous genes encoding these proteins have been fully sequences, and their phylogenetic ...
... In 1964, the three recognized activities of the PTS were presumed to correspond merely to three proteins. We now recognize dozens of PTS proteins in E. coli as well as hundreds of PTS proteins in other bacteria. Numerous genes encoding these proteins have been fully sequences, and their phylogenetic ...
Protein sorting at the ER–Golgi interface
... vesicles mediate the retrieval of escaped ER resident proteins and transport machinery that cycles continuously between the ER and Golgi. The COPI complex, also known as coatomer, consists of seven subunits (α, β, β', γ, δ, ε, and ζ-COP), which are recruited as an intact complex to membranes (Hara-K ...
... vesicles mediate the retrieval of escaped ER resident proteins and transport machinery that cycles continuously between the ER and Golgi. The COPI complex, also known as coatomer, consists of seven subunits (α, β, β', γ, δ, ε, and ζ-COP), which are recruited as an intact complex to membranes (Hara-K ...
Protein quality control and elimination of protein waste: The role of
... functions are manifold: signaling, movement, transport, membrane fusion, cell protection, regulation or catalysis are only some of them [1]. The three-dimensional structure that proteins acquire after ribosomal synthesis of their amino acid chain is crucial to their function. This function has to be ...
... functions are manifold: signaling, movement, transport, membrane fusion, cell protection, regulation or catalysis are only some of them [1]. The three-dimensional structure that proteins acquire after ribosomal synthesis of their amino acid chain is crucial to their function. This function has to be ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.