Biological Membrane Structure By Solid-State NMR
... and biological membranes. Different approaches have been developed to study these systems in which the restricted molecular motions result in broad NMR spectra. This contribution will first present an overview of the different techniques used to study lipid bilayers, namely 31P, 2H and 13C solid-sta ...
... and biological membranes. Different approaches have been developed to study these systems in which the restricted molecular motions result in broad NMR spectra. This contribution will first present an overview of the different techniques used to study lipid bilayers, namely 31P, 2H and 13C solid-sta ...
Genome-wide Screen for Inner Nuclear Membrane
... nuclear pore complexes (NPC), the nucleus contains other subnuclear structures including the nucleolus, cajal bodies, speckles, gems, and the nuclear matrix (Review: LAMOND and SLEEMAN 2003). Because membranes do not separate the various subnuclear compartments from one another, their structure, bio ...
... nuclear pore complexes (NPC), the nucleus contains other subnuclear structures including the nucleolus, cajal bodies, speckles, gems, and the nuclear matrix (Review: LAMOND and SLEEMAN 2003). Because membranes do not separate the various subnuclear compartments from one another, their structure, bio ...
PDF
... readily detected in the exocoelomic fluid and within 15min of injection in the case of that conceptus nearest the injection site. Entry into the exocoel was clearly by way of the paraplacental chorion, as evidenced by diffuse fluorescence around the trophoblastic giant cells and cytotrophoblast, and ...
... readily detected in the exocoelomic fluid and within 15min of injection in the case of that conceptus nearest the injection site. Entry into the exocoel was clearly by way of the paraplacental chorion, as evidenced by diffuse fluorescence around the trophoblastic giant cells and cytotrophoblast, and ...
Characterization of a novel phosphatidylinositol 3
... nih.gov) using the BLAST program [20]. Interrogation of the human genome sequences at the NCBI show that the gene sequence resides on chromosome 14. A matching EST sequence ( joint-database accession number AA399630) from a human testis library was found. The corresponding clone (ID 729901) harbouri ...
... nih.gov) using the BLAST program [20]. Interrogation of the human genome sequences at the NCBI show that the gene sequence resides on chromosome 14. A matching EST sequence ( joint-database accession number AA399630) from a human testis library was found. The corresponding clone (ID 729901) harbouri ...
Chapter 10
... targeted for binding to the ER membrane by the amino-acid sequence of the polypeptide chain being synthesized, rather than by intrinsic properties of the ribosome itself. Free and membrane-bound ribosomes are functionally indistinguishable, and protein synthesis generally initiates on ribosomes that ...
... targeted for binding to the ER membrane by the amino-acid sequence of the polypeptide chain being synthesized, rather than by intrinsic properties of the ribosome itself. Free and membrane-bound ribosomes are functionally indistinguishable, and protein synthesis generally initiates on ribosomes that ...
Bimolecular fluorescence complementation (BiFC)
... method has the technical disadvantage of drastically lower detectable signal levels of complemented fluorophores. The intensities resulting from protein-interaction complementations typically reach only about 1-10% when compared to the intact fluorophores. This, however, is not the result of a lower ...
... method has the technical disadvantage of drastically lower detectable signal levels of complemented fluorophores. The intensities resulting from protein-interaction complementations typically reach only about 1-10% when compared to the intact fluorophores. This, however, is not the result of a lower ...
The presence of monoglucosylated N196
... Glycosylation is an important cellular modification, and it is related to many human diseases and developmental defects [1]. It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such a ...
... Glycosylation is an important cellular modification, and it is related to many human diseases and developmental defects [1]. It introduces diversity into a biological system because of its inherent structural heterogeneity, and thus plays critical roles during a variety of cellular processes, such a ...
Heart Failure
... by the proteasome. If accumulation of ubiquitinated proteins outpaces proteasomal degradation, buildup of intracellular ubiquitinated protein occurs, resulting in formation of protein aggregates, large heterogeneous complexes that are poor substrates for proteasome-mediated proteolysis.17 To test fo ...
... by the proteasome. If accumulation of ubiquitinated proteins outpaces proteasomal degradation, buildup of intracellular ubiquitinated protein occurs, resulting in formation of protein aggregates, large heterogeneous complexes that are poor substrates for proteasome-mediated proteolysis.17 To test fo ...
Role of Streptococcus sanguinis sortase A in bacterial
... 10557. Lane 4: S. pyogenes strain SSI-1. Strain SSI-1 is known to express the Fn-binding protein FbaB [16]. ...
... 10557. Lane 4: S. pyogenes strain SSI-1. Strain SSI-1 is known to express the Fn-binding protein FbaB [16]. ...
Zinc finger family
... linkers, wrap smoothly around the major groove like those of Zif268 In contrast, fingers 4–5–6 form an open, extended structure running along one side of the DNA. Of these, only finger 5 makes contacts with bases in the major groove. The flanking fingers, 4 and 6, appear to serve primarily as spac ...
... linkers, wrap smoothly around the major groove like those of Zif268 In contrast, fingers 4–5–6 form an open, extended structure running along one side of the DNA. Of these, only finger 5 makes contacts with bases in the major groove. The flanking fingers, 4 and 6, appear to serve primarily as spac ...
A Nascent Membrane Protein Is Located Adjacent to ER Membrane
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
A Nascent Membrane Protein Is Located Adjacent to
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
... Lingappa, 1986). In contrast, the nonpolar transmembrane segments of membrane proteins (also termed "stop-transfer" sequences; Blobel, 1980) do not pass through the bilayer, but are integrated into the membrane. The stop-transfer sequence anchors the nascent polypeptide in the plane of the membrane ...
Strategy for Nonenveloped Virus Entry
... ascribed to ISVP-like particles, membrane disruption in vitro and membrane penetration within cells, are linked to N-myristoylated outer-capsid protein 1, present in 600 copies at the surfaces of ISVPs. To understand how 1 fulfills its role as the reovirus penetration protein, we monitored changes ...
... ascribed to ISVP-like particles, membrane disruption in vitro and membrane penetration within cells, are linked to N-myristoylated outer-capsid protein 1, present in 600 copies at the surfaces of ISVPs. To understand how 1 fulfills its role as the reovirus penetration protein, we monitored changes ...
Review Antibody flexibility observed in antigen binding and its
... and the conformational change of antibodies induced upon antigen binding, which is critical for intracellular signaling. In this article, I review several reports, including our recent results of dynamic structural analyses for antibodies. These reports strongly suggest the difficulty in interpretin ...
... and the conformational change of antibodies induced upon antigen binding, which is critical for intracellular signaling. In this article, I review several reports, including our recent results of dynamic structural analyses for antibodies. These reports strongly suggest the difficulty in interpretin ...
the scf ubiquitin ligase: insights into a molecular machine
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
... (LRR)), the domain is an arc-shaped α–β-repeat structure that is also found in many protein-binding contexts, including the extracellular-binding domain of certain surface receptors11,12. In most cases, FBLs also seem to involve substrate phosphorylation for their interaction, but this does not seem ...
PDF
... For this study, we used the consensus site, R⫺3-R⫺2-x⫺1-S兾TB⫹1, that had been defined by previous work with PKA enzymes from a variety of sources, including yeast and humans (19–22). A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, whi ...
... For this study, we used the consensus site, R⫺3-R⫺2-x⫺1-S兾TB⫹1, that had been defined by previous work with PKA enzymes from a variety of sources, including yeast and humans (19–22). A search of the S. cerevisiae proteome found 553 occurrences of this consensus sequence in 491 proteins (Table 2, whi ...
Assembly and disassembly of the influenza C matrix protein layer on
... Influenza is an RNA virus of the orthomyxovirus family. Like all viruses it infects healthy cells, reproduces in them, and then spreads to other cells. For the virus to spread from infected cells to healthy ones, the infected cell needs to produce a virion. The virion is a extracellular vesicle, in ...
... Influenza is an RNA virus of the orthomyxovirus family. Like all viruses it infects healthy cells, reproduces in them, and then spreads to other cells. For the virus to spread from infected cells to healthy ones, the infected cell needs to produce a virion. The virion is a extracellular vesicle, in ...
Translation
... acid, and the two ribosomal subunits • First, a small ribosomal subunit binds with mRNA and a special initiator tRNA • Then the small subunit moves along the mRNA until it reaches the start codon (AUG) • Proteins called initiation factors bring in the large subunit that completes the translation ...
... acid, and the two ribosomal subunits • First, a small ribosomal subunit binds with mRNA and a special initiator tRNA • Then the small subunit moves along the mRNA until it reaches the start codon (AUG) • Proteins called initiation factors bring in the large subunit that completes the translation ...
Small-molecule binding sites to explore new targets in the cancer
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
... The transcription factor HNF4A forms a homodimer complex to interact with DNA to control the expression of other genes. In the monomer structure, two binding sites were detected on the protein surface (Fig. S5D). One of these two sites is bound to a saturated fatty acid in multiple superimposed crys ...
Journal of Bacteriology
... Since only Cys32 and Cys34 are required to form the type 1 metal binding site in ArsR, one has to predict that As-S2 is sufficient to produce the desired conformational change. A type 1 metal binding site is also present in CadC as Cys58 and Cys60. However, CadC has an N-terminal extension containin ...
... Since only Cys32 and Cys34 are required to form the type 1 metal binding site in ArsR, one has to predict that As-S2 is sufficient to produce the desired conformational change. A type 1 metal binding site is also present in CadC as Cys58 and Cys60. However, CadC has an N-terminal extension containin ...
Essential Bioinformatics and Biocomputing
... resource in support of biological research. 2. We can learn much about a particular molecule by searching databases and using available analysis tools 3. A large number of databases are available for that task. Some databases are very general, some are more specialized, while some are very specializ ...
... resource in support of biological research. 2. We can learn much about a particular molecule by searching databases and using available analysis tools 3. A large number of databases are available for that task. Some databases are very general, some are more specialized, while some are very specializ ...
Advanced
... selecting and showing them atoms/bonds view. Menu Favorites … Sequences … Show sequences for 1ema chain A and 1g7k chain A (select both with shift pressed). Select residue 215E in 1g7k chain A (DsRed structure), Show atoms/bonds. Now select residue and 95R and Show atoms/bonds. Similarly select resi ...
... selecting and showing them atoms/bonds view. Menu Favorites … Sequences … Show sequences for 1ema chain A and 1g7k chain A (select both with shift pressed). Select residue 215E in 1g7k chain A (DsRed structure), Show atoms/bonds. Now select residue and 95R and Show atoms/bonds. Similarly select resi ...
Poster
... and understanding how electrons are transported in photosynthesis. Many of the studies conducted with hole burning spectroscopy are not simply to find out more about myoglobin, but are also meant to test out the technique. Someday, hole burning spectroscopy might be used on more proteins, and electr ...
... and understanding how electrons are transported in photosynthesis. Many of the studies conducted with hole burning spectroscopy are not simply to find out more about myoglobin, but are also meant to test out the technique. Someday, hole burning spectroscopy might be used on more proteins, and electr ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.