Regulation of microtubule dynamics by microtubule
... However the molecular mechanisms responsible tor the MAPstimulated microtubule stabilization are still unclear. One possibility is that the binding of MAPs to the microtubule lattice would simply diminish the rate of loss of tubulin from the polymer. Additionally, the binding of MAPs may increase th ...
... However the molecular mechanisms responsible tor the MAPstimulated microtubule stabilization are still unclear. One possibility is that the binding of MAPs to the microtubule lattice would simply diminish the rate of loss of tubulin from the polymer. Additionally, the binding of MAPs may increase th ...
Signal Sequences Specify the Targeting Route to the Endoplasmic
... Construction of pDNl06 and pDNl07. The reporter gene termed CU was constructed by fusingsequences encoding the amino-terminal 110 amino acids of PRC1 (CPY) and its promoter in frame to URA3 with glycine replacing the Ura3p initiator methionine as a linker~ This recombinant gene was inserted in the c ...
... Construction of pDNl06 and pDNl07. The reporter gene termed CU was constructed by fusingsequences encoding the amino-terminal 110 amino acids of PRC1 (CPY) and its promoter in frame to URA3 with glycine replacing the Ura3p initiator methionine as a linker~ This recombinant gene was inserted in the c ...
Hsp70 and Hsp90 of E. coli Directly Interact for Collaboration in
... conformations such as the open or the closed state of Hsp90. For example, Hop/Sti1 interacts simultaneously with Hsp70 and Hsp90 through its multiple tetratricopeptide repeat domains and facilitates substrate transfer from Hsp70 to Hsp90 by stabilizing the open conformation of Hsp90 [18,19]. The bac ...
... conformations such as the open or the closed state of Hsp90. For example, Hop/Sti1 interacts simultaneously with Hsp70 and Hsp90 through its multiple tetratricopeptide repeat domains and facilitates substrate transfer from Hsp70 to Hsp90 by stabilizing the open conformation of Hsp90 [18,19]. The bac ...
Efficiency assay of detergent removal columns on - G
... and suppress peptide ionization when analyzed by mass spectrometry. New (GB‐S10) detergent removal columns from G‐Biosciences were tested for their efficiency to remove anionic, nonionic or zwitterionic detergents (e.g. SDS, TritonX100 or CHAPS) from protein and tryptic peptide samples with minimal ...
... and suppress peptide ionization when analyzed by mass spectrometry. New (GB‐S10) detergent removal columns from G‐Biosciences were tested for their efficiency to remove anionic, nonionic or zwitterionic detergents (e.g. SDS, TritonX100 or CHAPS) from protein and tryptic peptide samples with minimal ...
Streptomycin Poster
... the first cure found for tuberculosis, was isolated from the soil organism Streptomyces griseus in 1943. We are using rapid prototyping technology to model the interaction between streptomycin and the 30S bacterial ribosomal subunit of RNA. This antibiotic binds tightly to bacterial 16S rRNA, causin ...
... the first cure found for tuberculosis, was isolated from the soil organism Streptomyces griseus in 1943. We are using rapid prototyping technology to model the interaction between streptomycin and the 30S bacterial ribosomal subunit of RNA. This antibiotic binds tightly to bacterial 16S rRNA, causin ...
Chapter 1: Biological Introduction: RING domain proteins
... the ubiquitination of the substrate. At least three different classes of E3 ligases have been found that mediate substrate ubiquitination. These E3 enzymes differ in the domain that recognizes the E2 enzymes, which can be a RING, a PHD (plant homeodomain) or a HECT (Homologous to E6AP COOH terminus) ...
... the ubiquitination of the substrate. At least three different classes of E3 ligases have been found that mediate substrate ubiquitination. These E3 enzymes differ in the domain that recognizes the E2 enzymes, which can be a RING, a PHD (plant homeodomain) or a HECT (Homologous to E6AP COOH terminus) ...
Identification of Pexl3p, a Peroxisomal Membrane Receptor for the
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
... are imported posttranslationally (for review see Lazarow and Fujiki, 1985). Import of peroxisomal matrix proteins requires both ATP and cytosolic factors (Imanaka et al., 1987; Wendland and Subramani, 1993), and recent evidence suggests that peroxisomal proteins can be imported in a folded state (Mc ...
Myoglobin and Hemoglobin - UF Macromolecular Structure Group
... During periods of oxygen deprivation oxymyoglobin releases its bound oxygen which is then used for metabolic purposes The tertiary structure of myoglobin is that of a typical water soluble globular protein Its secondary structure is unusual in that it contains a very high proportion (75%) of α-helic ...
... During periods of oxygen deprivation oxymyoglobin releases its bound oxygen which is then used for metabolic purposes The tertiary structure of myoglobin is that of a typical water soluble globular protein Its secondary structure is unusual in that it contains a very high proportion (75%) of α-helic ...
Promiscuity of the Euonymus Carbohydrate-Binding Domain
... of lectins led to the hypothesis that lectin-mediated protein-glycoconjugate interactions are essential for some important cellular processes in Embryophyta [9–11]. It is hypothesized that EUL proteins can help the plant to cope with a lot of environmental stresses and improve the stress tolerance i ...
... of lectins led to the hypothesis that lectin-mediated protein-glycoconjugate interactions are essential for some important cellular processes in Embryophyta [9–11]. It is hypothesized that EUL proteins can help the plant to cope with a lot of environmental stresses and improve the stress tolerance i ...
tRNA - U of L Class Index
... similar amino acids are encoded by similar codons ⇒ Change of 1 base has only small effect on protein ...
... similar amino acids are encoded by similar codons ⇒ Change of 1 base has only small effect on protein ...
Molecular Microbiology
... 2000) and transiently expressed in N. benthamiana through Agrobacterium-mediated infiltration to examine their cell death-eliciting activities. We have previously shown that HopZ1a and HopZ1b both trigger HR in N. benthamiana. However, they appear to induce defence responses through different pathwa ...
... 2000) and transiently expressed in N. benthamiana through Agrobacterium-mediated infiltration to examine their cell death-eliciting activities. We have previously shown that HopZ1a and HopZ1b both trigger HR in N. benthamiana. However, they appear to induce defence responses through different pathwa ...
Affinity Chromatography
... attachment of ligands to spacer arms already present on the pre-activated affinity matrix, whereas the ‘pre-assembly’ approach uses conventional organic chemistry to modify the ligand with a suitably derivatized spacer arm, after which the whole assembly is coupled to the matrix. The solid-phase ass ...
... attachment of ligands to spacer arms already present on the pre-activated affinity matrix, whereas the ‘pre-assembly’ approach uses conventional organic chemistry to modify the ligand with a suitably derivatized spacer arm, after which the whole assembly is coupled to the matrix. The solid-phase ass ...
Protein secretion and surface display in Gram
... of precursors that have been translocated across the plasma membrane into the extracellular milieu. A simple argument in favour of protein transport channels across peptidoglycan is the finding that boiling staphylococci in hot SDS does not release membrane or lipoproteins from the murein sacculus [ ...
... of precursors that have been translocated across the plasma membrane into the extracellular milieu. A simple argument in favour of protein transport channels across peptidoglycan is the finding that boiling staphylococci in hot SDS does not release membrane or lipoproteins from the murein sacculus [ ...
Proteomics insights: proteins related to larval attachment and
... Proteomics tools and pipelines used in the study of marine larval biology are outlined in Figure 1. A list of studies on larval proteomics is shown in Table 1. In the majority of these studies, the larval proteome was characterized by gel-based (2-DE) or gelfree proteomics, followed by mass spectrom ...
... Proteomics tools and pipelines used in the study of marine larval biology are outlined in Figure 1. A list of studies on larval proteomics is shown in Table 1. In the majority of these studies, the larval proteome was characterized by gel-based (2-DE) or gelfree proteomics, followed by mass spectrom ...
Thai Sports Supplements Co., Ltd.
... time to take whey protein because your body has been fasting during the night and most people don’t eat enough protein with their breakfast. Mid afternoon is another good time to consume a protein supplement because many people take a long break between lunch and dinner. This is especially important ...
... time to take whey protein because your body has been fasting during the night and most people don’t eat enough protein with their breakfast. Mid afternoon is another good time to consume a protein supplement because many people take a long break between lunch and dinner. This is especially important ...
Systemic methods for capturing protein–lipid interactions (PDF
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
... • 25% of the identified lipid interaction proteome is enriched in drug targets, while 12% of total human proteome is drugged. lipid probes may preferentially interact with proteins that can bind other small molecule ligands ...
Primary Structure Determination (Sanger)
... and determine the amino acid composition of these smaller fragments. • 3. Identify the N-terminus and C-terminus in the parent peptide and in each fragment. • 4. Organize the information so that the sequences of small fragments can be overlapped to reveal the full sequence. ...
... and determine the amino acid composition of these smaller fragments. • 3. Identify the N-terminus and C-terminus in the parent peptide and in each fragment. • 4. Organize the information so that the sequences of small fragments can be overlapped to reveal the full sequence. ...
The proPO and clotting system in crustaceans
... binding proteins ŽGNBPs. have been discovered in insect hemolymph; a Periplaneta americana LPS binding protein ŽNatori and Kubo, 1996., hemolin, a bacterial surface binding protein belonging to the immunoglobulin superfamily ŽSun et al., 1990., a 50-kDa GNBP from the silkworm, B. mori ŽLee et al., 1 ...
... binding proteins ŽGNBPs. have been discovered in insect hemolymph; a Periplaneta americana LPS binding protein ŽNatori and Kubo, 1996., hemolin, a bacterial surface binding protein belonging to the immunoglobulin superfamily ŽSun et al., 1990., a 50-kDa GNBP from the silkworm, B. mori ŽLee et al., 1 ...
Tubular structures involved in movement of cowpea mosaic virus are
... 5 8 K / 4 8 K antibodies; the percentage of infected protoplasts showing fluorescent tubules was also determined (Table 1). The percentage o f infected protoplasts determined using anti-58K/48K antibodies was similar to that determined using anti-capsid antibodies. O f the infected protoplasts, 67 t ...
... 5 8 K / 4 8 K antibodies; the percentage of infected protoplasts showing fluorescent tubules was also determined (Table 1). The percentage o f infected protoplasts determined using anti-58K/48K antibodies was similar to that determined using anti-capsid antibodies. O f the infected protoplasts, 67 t ...
The standard procedure starts with a set of sequences
... distributions into weights. They attempt to characterize a protein family or domain over its entire length. With a profile covering conserved as well as divergent sequence regions, there is a chance to obtain a significant similarity scores. This possibility is taken into account by the quality eval ...
... distributions into weights. They attempt to characterize a protein family or domain over its entire length. With a profile covering conserved as well as divergent sequence regions, there is a chance to obtain a significant similarity scores. This possibility is taken into account by the quality eval ...
Introduction to NMR spectroscopy
... Depending on the length of the RF pulse, the bulk magnetisation of an ensemble of spins will flip at a different angle with respect to the static ...
... Depending on the length of the RF pulse, the bulk magnetisation of an ensemble of spins will flip at a different angle with respect to the static ...
The Genome-linked Protein of Picornaviruses. VIII. Complete Amino
... Nomoto et aL, 1977), and then immediately subjected to automated Edman degradation. Digestion was at 37 °C for 30 rain at an enzyme/RNA ratio of 2 units/40 ~tg RNA. VPg RNA and P3-9 were digested with carboxypeptidase-A (Worthington Biochemicals) following the procedure of Bhown et al. (1980) as mod ...
... Nomoto et aL, 1977), and then immediately subjected to automated Edman degradation. Digestion was at 37 °C for 30 rain at an enzyme/RNA ratio of 2 units/40 ~tg RNA. VPg RNA and P3-9 were digested with carboxypeptidase-A (Worthington Biochemicals) following the procedure of Bhown et al. (1980) as mod ...
C3 receptors on macrophages - Journal of Cell Science
... zymosan, a value of 10% is generally accepted. Both fluid-phase and surface-bound C3b are cleaved by factor I in the presence of cofactors. To date, three molecules are known to have the cofactor activity for this reaction. They are factor H (previously known as the /3lH-globulin) (Whaley & Ruddy, I ...
... zymosan, a value of 10% is generally accepted. Both fluid-phase and surface-bound C3b are cleaved by factor I in the presence of cofactors. To date, three molecules are known to have the cofactor activity for this reaction. They are factor H (previously known as the /3lH-globulin) (Whaley & Ruddy, I ...
Intrinsically disordered proteins
An intrinsically disordered protein (IDP) is a protein that lacks a fixed or ordered three-dimensional structure. IDPs cover a spectrum of states from fully unstructured to partially structured and include random coils, (pre-)molten globules, and large multi-domain proteins connected by flexible linkers. They constitute one of the main types of protein (alongside globular, fibrous and membrane proteins).The discovery of IDPs has challenged the traditional protein structure paradigm, that protein function depends on a fixed three-dimensional structure. This dogma has been challenged over the last decades by increasing evidence from various branches of structural biology, suggesting that protein dynamics may be highly relevant for such systems. Despite their lack of stable structure, IDPs are a very large and functionally important class of proteins. In some cases, IDPs can adopt a fixed three-dimensional structure after binding to other macromolecules.