Omni-Tube PCR, PCR Reaction,Digest, Ligation, and the 3
... he Omni-Tube PCR system is designed and optimized for performing PCR, digestion, ligation, and purification all in a single tube. No extra tubes, spin-columns, filter plates, silica membrane, magnetic beads, vacuum or filtration steps are needed for purification, and no transfer of reaction mixtures ...
... he Omni-Tube PCR system is designed and optimized for performing PCR, digestion, ligation, and purification all in a single tube. No extra tubes, spin-columns, filter plates, silica membrane, magnetic beads, vacuum or filtration steps are needed for purification, and no transfer of reaction mixtures ...
CSA Biopharm - Moodle Lille 2
... Culture of the protein of interest with DCs.DCs take up the protein and process it HLA molecules present epitopes from the protein on the DC surface. Cells are lyzedand HLApeptide molecules are then recovered in an immune affinity step and analyzed by sequencing mass spectrometry. Peptides are recov ...
... Culture of the protein of interest with DCs.DCs take up the protein and process it HLA molecules present epitopes from the protein on the DC surface. Cells are lyzedand HLApeptide molecules are then recovered in an immune affinity step and analyzed by sequencing mass spectrometry. Peptides are recov ...
A human SARS-CoV neutralizing antibody against epitope on S2
... subjected to 96-well plate coated with SARS-CoV particles and incubated for another 1 h at 37 C. HRP-conjugated anti-human Fc was added as secondary antibody. The following procedures were the same as described in ELISA. Antibody affinity assay. The binding kinetics of scFv B1 to SARSCoV was analyzed ...
... subjected to 96-well plate coated with SARS-CoV particles and incubated for another 1 h at 37 C. HRP-conjugated anti-human Fc was added as secondary antibody. The following procedures were the same as described in ELISA. Antibody affinity assay. The binding kinetics of scFv B1 to SARSCoV was analyzed ...
A Survey of Flexible Protein Binding Mechanisms and their
... partner proteins.35 Several physiological advantages have been suggested for the use of disordered proteins that only fold upon reaching their targets. One functional advantage is that natively unfolded proteins are more adaptive, giving them the capability to bind to several different targets,31,36 ...
... partner proteins.35 Several physiological advantages have been suggested for the use of disordered proteins that only fold upon reaching their targets. One functional advantage is that natively unfolded proteins are more adaptive, giving them the capability to bind to several different targets,31,36 ...
Whey and Glutathione - Part III
... protein. It contains little if any fat or lactose. "Whey protein concentrate" will vary between 25-89% protein. "Whey protein concentrate" at an 80% protein content is the form most readily available as a protein powder supplement. Hydrolyzed Whey Protein Hydrolysis breaks the protein chains down in ...
... protein. It contains little if any fat or lactose. "Whey protein concentrate" will vary between 25-89% protein. "Whey protein concentrate" at an 80% protein content is the form most readily available as a protein powder supplement. Hydrolyzed Whey Protein Hydrolysis breaks the protein chains down in ...
DNA-Directed Antibody Immobilization for
... masking the antigen binding sites due to multiple covalent interactions between the antibody and the surface.2,3 In addition, some antibodies might lose their activity due to steric hindrance by the surface and adjacent antibody molecules.4 Moreover, issues such as nonuniform spot morphologies and v ...
... masking the antigen binding sites due to multiple covalent interactions between the antibody and the surface.2,3 In addition, some antibodies might lose their activity due to steric hindrance by the surface and adjacent antibody molecules.4 Moreover, issues such as nonuniform spot morphologies and v ...
innovative development strategies and applications for bispecific
... is label-free, allows users to view the dynamics of bispecific antibody binding and dissociation events with two targets. SPR systems such as Biacore (GE Healthcare) are label free, the readout is continuous, and data analysis can be done at multiple time points, or by fitting entire binding curves ...
... is label-free, allows users to view the dynamics of bispecific antibody binding and dissociation events with two targets. SPR systems such as Biacore (GE Healthcare) are label free, the readout is continuous, and data analysis can be done at multiple time points, or by fitting entire binding curves ...
Zika Virus Proteins - Peptides International
... like the other flaviviruses, Zika virus is envelthe CDC, however it is not yet known whethoped and icosahedral and has a nonsegmented, single- er Zika virus causes microcephaly. Furthermore, a constranded, positive-sense RNA genome. Zika fever is an nection has been established with neurologic condi ...
... like the other flaviviruses, Zika virus is envelthe CDC, however it is not yet known whethoped and icosahedral and has a nonsegmented, single- er Zika virus causes microcephaly. Furthermore, a constranded, positive-sense RNA genome. Zika fever is an nection has been established with neurologic condi ...
BEBPA 2015 Nab Conference Brochure
... requirements of these assays for sensitivity, precision, robustness, retreatment with therapeutic monoclonal antibodies can induce anti-drug producibility and the need for matrix (serum/plasma) tolerance make antibody (ADA) formation, also known as immunogenicity, which is asso- this an even more ch ...
... requirements of these assays for sensitivity, precision, robustness, retreatment with therapeutic monoclonal antibodies can induce anti-drug producibility and the need for matrix (serum/plasma) tolerance make antibody (ADA) formation, also known as immunogenicity, which is asso- this an even more ch ...
Monoclonal antibodies - Federation of American Societies for
... the pool of antibodies found in the blood had an infinite ability to combine with any substance, yet superficially seemed to be a nearly uniform group of proteins. This confounded him and bolstered his determination to decipher antibody structure and function. In the late 1950’s, he took an innovati ...
... the pool of antibodies found in the blood had an infinite ability to combine with any substance, yet superficially seemed to be a nearly uniform group of proteins. This confounded him and bolstered his determination to decipher antibody structure and function. In the late 1950’s, he took an innovati ...
INPS: predicting the impact of non-synonymous variations on protein
... The available methods were trained under different conditions and on different datasets. They address three different questions. Briefly, they can: (i) predict the DDG real values (in regression) upon residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrea ...
... The available methods were trained under different conditions and on different datasets. They address three different questions. Briefly, they can: (i) predict the DDG real values (in regression) upon residue substitution, (ii) predict whether a residue substitution promotes a DDG increase or decrea ...
A VLP-based Platform for Vaccine Discovery
... These are the results of a single round of selection. Although the consensus sequence matches that of the wildtype epitope, no member of this family is itself a perfect match. We suspect these are relatively low affinity ligands, isolated as a result of our inability to distinguish intrinsically tig ...
... These are the results of a single round of selection. Although the consensus sequence matches that of the wildtype epitope, no member of this family is itself a perfect match. We suspect these are relatively low affinity ligands, isolated as a result of our inability to distinguish intrinsically tig ...
Antigen-Antibody Interaction
... soluble antibody of the same specificity as the cells receptor, and (2) remembering and refining the cells receptor as a clone of cells for future exposure to the antigen. A single has many Ig receptors, as many as 104 or 105 [7], [13] (page 34). Not all B-lymphocyte have surface bound Ig (typically ...
... soluble antibody of the same specificity as the cells receptor, and (2) remembering and refining the cells receptor as a clone of cells for future exposure to the antigen. A single has many Ig receptors, as many as 104 or 105 [7], [13] (page 34). Not all B-lymphocyte have surface bound Ig (typically ...
Overview of tag protein fusions
... recombinant proteins are well-recognized. Nevertheless, it is difficult to choose the right purification system for a specific protein of interest. This review gives an overview of the most frequently used and interesting systems: Argtag, calmodulin-binding peptide, cellulose-binding domain, DsbA, c ...
... recombinant proteins are well-recognized. Nevertheless, it is difficult to choose the right purification system for a specific protein of interest. This review gives an overview of the most frequently used and interesting systems: Argtag, calmodulin-binding peptide, cellulose-binding domain, DsbA, c ...
Pvlea-18, a Member of a New Late-Embryogenesis
... We extracted the proteins from fresh plant tissues by the TCA/acetone method, as described above. Different dilutions of the total protein extract were separated in 12% SDS-PAGE and transferred to nitrocellulose. We included different known amounts of purified GST-PvLEA fusion protein as an internal ...
... We extracted the proteins from fresh plant tissues by the TCA/acetone method, as described above. Different dilutions of the total protein extract were separated in 12% SDS-PAGE and transferred to nitrocellulose. We included different known amounts of purified GST-PvLEA fusion protein as an internal ...
Linking folding and binding
... induced folding or conformational selection (Figure 2). In the first mechanism, the protein associates with its binding partner in a fully disordered state and subsequently folds in association with the target protein; folding is induced by association with the target. In the conformational selectio ...
... induced folding or conformational selection (Figure 2). In the first mechanism, the protein associates with its binding partner in a fully disordered state and subsequently folds in association with the target protein; folding is induced by association with the target. In the conformational selectio ...
Axonal Transport of Monoclonal Antibodies
... sucrose. Transported mouse immunoglobulins were localized in frozen sections with a modification of the peroxidase-antiperoxidase (PAP) method of Stemberger (1978), by the procedure detailed previously (Ritchie et al., 1985b), using goat anti-mouse IgG (heavy and light chains specific, without cross ...
... sucrose. Transported mouse immunoglobulins were localized in frozen sections with a modification of the peroxidase-antiperoxidase (PAP) method of Stemberger (1978), by the procedure detailed previously (Ritchie et al., 1985b), using goat anti-mouse IgG (heavy and light chains specific, without cross ...
Docking of B-cell epitope antigen to specific hepatitis B antibody
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
... antigen and in understanding where important regions of an antigen are located in its three-dimensional structure. Therefore, mapping the interaction surface between antibody and antigen that bind each other is of outstanding interest in molecular immunology. Complementary to various experimental te ...
Antibody Conjugates with Unnatural Amino Acids
... acids, must be efficiently expressed. Third, an engineered tRNA, which serves as a substrate for the engineered tRNA synthetase, must also be coexpressed. This orthogonal reactivity of the RS/ tRNA pair is crucial for effective uAA incorporation in the absence of unwanted background incorporation of na ...
... acids, must be efficiently expressed. Third, an engineered tRNA, which serves as a substrate for the engineered tRNA synthetase, must also be coexpressed. This orthogonal reactivity of the RS/ tRNA pair is crucial for effective uAA incorporation in the absence of unwanted background incorporation of na ...
Monoclonal Anti-human IL-18 BP Antibody Catalogue Number
... Centrifuge the vial before opening to prevent loss of the powder. ...
... Centrifuge the vial before opening to prevent loss of the powder. ...
chordin (H-300): sc-28964 - Santa Cruz Biotechnology
... chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGFβ-like bone morphogenetic proteins and sequestering them in latent complexes. Chordin dorsalizes early vertebrate embryonic tissues by binding to bone morphogenetic protei ...
... chordin. Chordin is a key developmental protein that dorsalizes early vertebrate embryonic tissues by binding to ventralizing TGFβ-like bone morphogenetic proteins and sequestering them in latent complexes. Chordin dorsalizes early vertebrate embryonic tissues by binding to bone morphogenetic protei ...
NMR spectroscopy brings invisible protein states into
... rotations about unhindered dihedral angles16, to substantial chain displacements17. In some favorable cases such picosecond to nanosecond dynamics can be inferred from temperature factors derived from crystal structures18 or often more rigorously from NMR spin relaxation properties19–22. In addition ...
... rotations about unhindered dihedral angles16, to substantial chain displacements17. In some favorable cases such picosecond to nanosecond dynamics can be inferred from temperature factors derived from crystal structures18 or often more rigorously from NMR spin relaxation properties19–22. In addition ...
Principles of Nucleic Acid Separation by Agarose Gel Electrophoresis
... common methods for staining and visualization of DNA are described in details. Agarose gel electrophoresis provides multiple advantages that make it widely popular. For example, nucleic acids are not chemically altered during the size separation process and agarose gels can easily be viewed and hand ...
... common methods for staining and visualization of DNA are described in details. Agarose gel electrophoresis provides multiple advantages that make it widely popular. For example, nucleic acids are not chemically altered during the size separation process and agarose gels can easily be viewed and hand ...
Exploring Prostate Proteins and Antibodies
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...
... Which proteins are expressed in the prostate? To acknowledge Prostate Cancer Awareness Month, we take a closer look at the human prostate specific proteome. We explore the Human Protein Atlas's analysis of the prostate specific transcriptome, and present selected antibodies against proteins specific ...