Expanded protein information at SGD: new pages and proteome browser.
... describes the specific function of the protein when it is known. These fields have been recently reviewed and rewritten using a standard, consistent format so that they accurately reflect the current state of knowledge for each gene product. The references for this information are found at the botto ...
... describes the specific function of the protein when it is known. These fields have been recently reviewed and rewritten using a standard, consistent format so that they accurately reflect the current state of knowledge for each gene product. The references for this information are found at the botto ...
In immunology, an adjuvant is an agent that may stimulate the
... biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding. An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of ...
... biochemical assays. Other assays measure processes such as enzyme activity, antigen capture, stem cell activity, and competitive protein binding. An immunoassay is a biochemical test that measures the presence or concentration of a substance in solutions that frequently contain a complex mixture of ...
PDF Format - Kinexus Bioinformatics Corporation
... Over 800 different commercial antibodies sourced from over 25 vendors are printed on the Kinex™ Antibody Microarray chip. These antibodies have been tested in-house at Kinexus and shown to perform on Western blots and proto-type microarrays to bind to their intended target proteins. These include a ...
... Over 800 different commercial antibodies sourced from over 25 vendors are printed on the Kinex™ Antibody Microarray chip. These antibodies have been tested in-house at Kinexus and shown to perform on Western blots and proto-type microarrays to bind to their intended target proteins. These include a ...
Methods for identifying microRNA binding motifs
... • Reduces background as the strong streptavidin binding affords the use of harsher (but more effective) washing conditions ...
... • Reduces background as the strong streptavidin binding affords the use of harsher (but more effective) washing conditions ...
Replacing Traditional SDS-PAGE and Bradford Techniques
... user. It is therefore very convenient and allows the user to focus immediately on the samples of interest. There is no need to look at all the analyses in detail. The result-flagging rules can also be applied across several “jobs” in the database, for example, in order to monitor processes over time ...
... user. It is therefore very convenient and allows the user to focus immediately on the samples of interest. There is no need to look at all the analyses in detail. The result-flagging rules can also be applied across several “jobs” in the database, for example, in order to monitor processes over time ...
HuCAL® Antibodies Technical Manual Introduction to Recombinant
... Over the last three decades the use of antibodies has increased greatly, both as tools for basic research and diagnostics and as therapeutic agents. This has been driven in part by advances in recombinant antibody technology. At the forefront of these advances is the Human Combinatorial Antibody Lib ...
... Over the last three decades the use of antibodies has increased greatly, both as tools for basic research and diagnostics and as therapeutic agents. This has been driven in part by advances in recombinant antibody technology. At the forefront of these advances is the Human Combinatorial Antibody Lib ...
Adobe PDF - CL Davis Foundation
... • The analyte mixtures B–F are defined by gray areas to indicate the parts of the ADC structure that would not be fully measured by the respective assay and colored areas to indicate information that would be fully determined. ...
... • The analyte mixtures B–F are defined by gray areas to indicate the parts of the ADC structure that would not be fully measured by the respective assay and colored areas to indicate information that would be fully determined. ...
Immunology Review
... When the zeta potential is lowered, the red cells can more closely approach each other, allowing antibodies to crosslink antigens between cells. ...
... When the zeta potential is lowered, the red cells can more closely approach each other, allowing antibodies to crosslink antigens between cells. ...
ELECTROPHORESIS
... 5. Smaller DNA molecules will travel faster as compared to larger ones. Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
... 5. Smaller DNA molecules will travel faster as compared to larger ones. Commonly used buffers are tris acetate, tris borate or tris phosphate at a concentration of 50mM over a pH range of 7.5-8.0. ...
Affinity and folding properties both influence the selection of
... times independently, and Table 1 gives the results how often each mutant was found. It can be seen that all mutants are found at comparable levels, but the three mutations with a large decrease in affinity and/or folding defects (His-H58-Ala, Arg-H95-Ala and Trp-L91-Ala) are immediately lost. On the ...
... times independently, and Table 1 gives the results how often each mutant was found. It can be seen that all mutants are found at comparable levels, but the three mutations with a large decrease in affinity and/or folding defects (His-H58-Ala, Arg-H95-Ala and Trp-L91-Ala) are immediately lost. On the ...
NMR Analysis of Protein
... • As we have seen before, line-width is directly related to apparent MW a small-molecule (~100-1,000Da) is orders of magnitude lighter than a typical protein (10s of KDa) a small molecule has sharp NMR line-widths (few Hz at most)) protein has broad line-widths (10s of Hz) if a small molecule ...
... • As we have seen before, line-width is directly related to apparent MW a small-molecule (~100-1,000Da) is orders of magnitude lighter than a typical protein (10s of KDa) a small molecule has sharp NMR line-widths (few Hz at most)) protein has broad line-widths (10s of Hz) if a small molecule ...
5.5_antibodies
... the simplest, is polyclonal. That is, the system manufactures antibodies for a great range of proteins found on the cell surface membrane. • Even if you were to isolate a single antibody-secreting cell, and place it in culture, it would die out after a few generations because of the limited growth p ...
... the simplest, is polyclonal. That is, the system manufactures antibodies for a great range of proteins found on the cell surface membrane. • Even if you were to isolate a single antibody-secreting cell, and place it in culture, it would die out after a few generations because of the limited growth p ...
CHAPTER 4 Proteins: Structure, Function, Folding
... • Conformational changes may occur upon ligand binding (Daniel Koshland in 1958) – This adaptation is called the induced fit – Induced fit allows for tighter binding of the ligand – Induced fit allows for high affinity for different ligands • Both the ligand and the protein can change their conforma ...
... • Conformational changes may occur upon ligand binding (Daniel Koshland in 1958) – This adaptation is called the induced fit – Induced fit allows for tighter binding of the ligand – Induced fit allows for high affinity for different ligands • Both the ligand and the protein can change their conforma ...
LKM-1 Autoantibodies Recognize a Short Linear Sequence
... the figure legends. After electrophoresis the protein content ofthe gels was transferred electrophoretically to nitrocellulose (12). The nitrocellulose filters were probed with antibody as described previously (3), and bound antibodies were detected as described in the legends to figures. Affinitypu ...
... the figure legends. After electrophoresis the protein content ofthe gels was transferred electrophoretically to nitrocellulose (12). The nitrocellulose filters were probed with antibody as described previously (3), and bound antibodies were detected as described in the legends to figures. Affinitypu ...
Rapid screening of PCR products using a novel agarose gel
... Fig 2. Comparison of separations on a large-format agarose gel vs. a Ready-To-Run gel. (A) 1 µl of a 1:10 dilution of PCR product separated in a 2% agarose gel containing SYBR Green I at 83 V for approximately 2 h. (B) 1 µl of a 1:5 dilution of PCR product separated in a 1.5% agarose Ready-To-Run ge ...
... Fig 2. Comparison of separations on a large-format agarose gel vs. a Ready-To-Run gel. (A) 1 µl of a 1:10 dilution of PCR product separated in a 2% agarose gel containing SYBR Green I at 83 V for approximately 2 h. (B) 1 µl of a 1:5 dilution of PCR product separated in a 1.5% agarose Ready-To-Run ge ...
Nugget
... The E. coli SSB protein is a helix-destabilizing protein that binds with high affinity to ssDNA intermediates during replication, recombination and DNA repair. It has been proposed that SSB, while bound to ssDNA, can translocate along the ssDNA via a rolling mechansim. The protein cannot translocate ...
... The E. coli SSB protein is a helix-destabilizing protein that binds with high affinity to ssDNA intermediates during replication, recombination and DNA repair. It has been proposed that SSB, while bound to ssDNA, can translocate along the ssDNA via a rolling mechansim. The protein cannot translocate ...
3P Color Buffer
... 10X P-Green Buffer The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that sep ...
... 10X P-Green Buffer The 10X P-Green Buffer allows you to go directly from the thermal cycler to gel analysis. The buffer contains a compound that increases sample density, so that samples sink easily into the wells of an agarose gel. The 10X P-Green Buffer contains two dyes (yellow and blue) that sep ...
Site-specific functionalization of proteins and their applications to
... scheme in figure ) [2 ]. Since then, many analogs have been synthesized and tested in auxotrophic bacterial hosts for incorporation at the expense of canonical amino acids [22]. The strict biological machinery accepts only minor modifications such as alkenes [23], alkynes [24] and azides [24] as ami ...
... scheme in figure ) [2 ]. Since then, many analogs have been synthesized and tested in auxotrophic bacterial hosts for incorporation at the expense of canonical amino acids [22]. The strict biological machinery accepts only minor modifications such as alkenes [23], alkynes [24] and azides [24] as ami ...
Sensitive Detection of Botulinum Neurotoxin Type A
... could be used to capture BoNT/A from complex matrices. Methods Used: Magnetic beads coated with a polyclonal antibody specific for BoNT/A heavy chain, List Prod #730, were used to capture and concentrate the toxin prior to addition of the substrate, FRET peptide SNAPtide®, List Prod #520. Various bu ...
... could be used to capture BoNT/A from complex matrices. Methods Used: Magnetic beads coated with a polyclonal antibody specific for BoNT/A heavy chain, List Prod #730, were used to capture and concentrate the toxin prior to addition of the substrate, FRET peptide SNAPtide®, List Prod #520. Various bu ...
Ouchterlony Procedure
... attach to mast cells and basophils. When antigens bind, they signal release of histamine. IgA - A dimer - Produced by cells in the mucus membranes to prevent attachment of pathogens. IgA is also found in many body secretions including milk. IgM - A pentamer - First antibody to appear following expos ...
... attach to mast cells and basophils. When antigens bind, they signal release of histamine. IgA - A dimer - Produced by cells in the mucus membranes to prevent attachment of pathogens. IgA is also found in many body secretions including milk. IgM - A pentamer - First antibody to appear following expos ...
Dr Asmat Salim MM707-electrophoresis 2014
... samples from a gel to a membrane and their subsequent detection on the surface of the membrane. • Western blotting (also called immunoblotting because an antibody is used to specifically detect its antigen) is now a routine technique for protein analysis. • The specificity of the antibody-antigen in ...
... samples from a gel to a membrane and their subsequent detection on the surface of the membrane. • Western blotting (also called immunoblotting because an antibody is used to specifically detect its antigen) is now a routine technique for protein analysis. • The specificity of the antibody-antigen in ...
2-D Quant Kit - GE Healthcare Life Sciences
... is essential. If the sample is viscous, it may be difficult to accurately pipette volumes below 10 µl. More accurate results may be obtained by diluting a concentrated sample into water prior to assaying. Performing the assay in duplicate is recommended. Unlike most protein assays, the absorbance of ...
... is essential. If the sample is viscous, it may be difficult to accurately pipette volumes below 10 µl. More accurate results may be obtained by diluting a concentrated sample into water prior to assaying. Performing the assay in duplicate is recommended. Unlike most protein assays, the absorbance of ...
SIZE EXCLUSION AND HI CHROMATOGRAPHY
... • The mixture of molecules is added to the column • Large molecules move through the column quickly traveling around the beads • Smaller molecules move through the pores of the beads and take longer to pass through the column ...
... • The mixture of molecules is added to the column • Large molecules move through the column quickly traveling around the beads • Smaller molecules move through the pores of the beads and take longer to pass through the column ...
Structural characterization of an antibody in complex with
... the exact role of its protease activity in ...
... the exact role of its protease activity in ...