Nucleic Acids Research
... interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface (6,7,8–11) In addition to nucleic acid binding, other essential protein functions could be dependent on the presenc ...
... interfaces (5–8). Recently, several methods have been developed for automatic prediction of DNA-binding proteins based on the existence of large positive patches on the protein surface (6,7,8–11) In addition to nucleic acid binding, other essential protein functions could be dependent on the presenc ...
ab115058 – Histone H3 (pan-methyl K4) Quantification Kit (Colorimetric)
... Any variation in operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding ...
... Any variation in operator, pipetting technique, washing technique, incubation time or temperature, and kit age can cause variation in binding ...
CLINICAL CHEMISTRY CHAPTER 5
... antibodies to a parallel trough along the separated proteins – The antibodies diffuse through the agar and form lines of precipitation with their respective antigens – The visible precipitant arcs can be compared to known standards to identify specific protein bands – Or to detect missing bands ...
... antibodies to a parallel trough along the separated proteins – The antibodies diffuse through the agar and form lines of precipitation with their respective antigens – The visible precipitant arcs can be compared to known standards to identify specific protein bands – Or to detect missing bands ...
OptCDR: a general computational method for the design
... user-specified size of antibody CDRs that can be grafted into the framework of an antibody using standard humanization techniques (Almagro and Fransson, 2008). Benchmarking against three test cases reveals OptCDR’s ability to expediently generate multiple original and diverse antibody libraries with ...
... user-specified size of antibody CDRs that can be grafted into the framework of an antibody using standard humanization techniques (Almagro and Fransson, 2008). Benchmarking against three test cases reveals OptCDR’s ability to expediently generate multiple original and diverse antibody libraries with ...
Introduction to Immunoassays
... human would - as a defense mechanism when exposed to an antigen. • Antiserum contains a mixture of antibodies, each of which may bind to different antigen binding sites, or epitopes. ...
... human would - as a defense mechanism when exposed to an antigen. • Antiserum contains a mixture of antibodies, each of which may bind to different antigen binding sites, or epitopes. ...
Serological tests
... to non-specific adsorption, it is important for the total protein concentration to be similar to that of the antigen standards. 3. A concentrated solution of non-interacting protein, such as Bovine Serum Albumin (BSA) or casein, is added to all plate wells. This step is known as blocking, because th ...
... to non-specific adsorption, it is important for the total protein concentration to be similar to that of the antigen standards. 3. A concentrated solution of non-interacting protein, such as Bovine Serum Albumin (BSA) or casein, is added to all plate wells. This step is known as blocking, because th ...
UltraClean 15 DNA Purification Kit
... 7. Incubate 5 minutes at room temperature. Important: Mix several times during this binding step by flicking the tube for small volumes or shaking and inverting for large volumes. 8. Centrifuge 5 seconds. Remove supernatant and set aside. (If DNA does not bind, it can be recovered. See Hints and Tro ...
... 7. Incubate 5 minutes at room temperature. Important: Mix several times during this binding step by flicking the tube for small volumes or shaking and inverting for large volumes. 8. Centrifuge 5 seconds. Remove supernatant and set aside. (If DNA does not bind, it can be recovered. See Hints and Tro ...
Horizontal, agarose systems
... E-Gel® EX gels were developed for ultimate sensitivity and demonstrate over 5-fold greater sensitivity than comparable gels containing ethidium bromide. Quick check of RNA samples E-Gel® EX gels can also be used for fast analysis of RNA samples to check on their integrity before proceeding with down ...
... E-Gel® EX gels were developed for ultimate sensitivity and demonstrate over 5-fold greater sensitivity than comparable gels containing ethidium bromide. Quick check of RNA samples E-Gel® EX gels can also be used for fast analysis of RNA samples to check on their integrity before proceeding with down ...
- SlideBoom
... • It absorbs faster in your body and helps in building muscles • It contains amino acids which helps in muscle recovery faster ...
... • It absorbs faster in your body and helps in building muscles • It contains amino acids which helps in muscle recovery faster ...
humanized antibodies - Assets - Cambridge
... animal mAbs are administered in multiple doses, the patient almost invariably raises an immune response to the mAbs causing attenuation of their biological activity and clinical symptoms similar to serum sickness and sometimes serious enough to endanger life. This anti-antibody response (AAR), also ...
... animal mAbs are administered in multiple doses, the patient almost invariably raises an immune response to the mAbs causing attenuation of their biological activity and clinical symptoms similar to serum sickness and sometimes serious enough to endanger life. This anti-antibody response (AAR), also ...
nCounter® Vantage 3D™ RNA:Protein Immune Cell Profiling Assay
... once, followed by blotting of the inverted plate on a fresh paper towel or lab wipe; OR ...
... once, followed by blotting of the inverted plate on a fresh paper towel or lab wipe; OR ...
Operon Models
... repressor protein. 2. Again, each end of the noodle/operon should feature an unlabeled/untaped section, to show the continuation of the DNA strand. 3. Wrap spirals of colored electrical tape (or shade the noodle with colored markers) where each of the 3 gene domain regions would be found (lac Z, lac ...
... repressor protein. 2. Again, each end of the noodle/operon should feature an unlabeled/untaped section, to show the continuation of the DNA strand. 3. Wrap spirals of colored electrical tape (or shade the noodle with colored markers) where each of the 3 gene domain regions would be found (lac Z, lac ...
A Method To Define the Carboxyl Terminal of Proteins
... is repeated a number of times to provide the sequence of the C-terminus.4-6 The relatively modest sensitivity attainable to date has limited the utility of this approach, although improvements are steadily being made.7,8 An alternative set of chemical procedures has been developed that utilize acid- ...
... is repeated a number of times to provide the sequence of the C-terminus.4-6 The relatively modest sensitivity attainable to date has limited the utility of this approach, although improvements are steadily being made.7,8 An alternative set of chemical procedures has been developed that utilize acid- ...
Texas Tech University Health Science Center School of Medicine
... C. Involves anti-immunoglobulin to cross link antibody bound to its specific receptor on red blood cells D. Involves anti-immunoglobulin coupled with an easily detectable enzyme binding to antibody bound to antigen attached to a solid surface (usually plastic) E. Involves an increase of phagocytosis ...
... C. Involves anti-immunoglobulin to cross link antibody bound to its specific receptor on red blood cells D. Involves anti-immunoglobulin coupled with an easily detectable enzyme binding to antibody bound to antigen attached to a solid surface (usually plastic) E. Involves an increase of phagocytosis ...
Characterization and application of monoclonal antibodies against
... mainly focus on detecting viral antigen-specific antibodies in the sera of suspected patients. Generally, the antibody response often develops on 10–14 days following SARS-CoV infection [3], so that the diagnosis based on a viral-specific Ab would miss a good time point for antiviral therapy effectivel ...
... mainly focus on detecting viral antigen-specific antibodies in the sera of suspected patients. Generally, the antibody response often develops on 10–14 days following SARS-CoV infection [3], so that the diagnosis based on a viral-specific Ab would miss a good time point for antiviral therapy effectivel ...
Function of Conserved Tryptophans in the Aspergillus niger
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
... In this paper, we have followed the binding site numbering convention of Lawson et al. (1994) in domain E of cyclodextrin glycosyltransferase. This is opposite to the numbering adopted by Klein and Schulz (1991) and Coutinho and Reilly (1994b). Production of the Variants. The three variants, W615K, ...
Antibody Engineering and Therapeutics 2016
... consist of complex mixtures of antibodies directed against distinct disease-related targets and epitopes. Antibody mixtures thereby provide an ability to target disease heterogeneity and plasticity and to prevent escape. Strikingly, most approved passive immunotherapy approaches (i.e., based on admi ...
... consist of complex mixtures of antibodies directed against distinct disease-related targets and epitopes. Antibody mixtures thereby provide an ability to target disease heterogeneity and plasticity and to prevent escape. Strikingly, most approved passive immunotherapy approaches (i.e., based on admi ...
Definition of a Conserved Immunodominant Domain on Hepatitis C
... domain B. Each HMAb broadly neutralizes retroviral pseudotype particles expressing HCV E1 and E2 glycoproteins, as well as the infectious chimeric genotype 1a and genotype 2a viruses. Alanine substitutions of residues within a region of E2 involved in binding to CD81 showed that critical E2 contact ...
... domain B. Each HMAb broadly neutralizes retroviral pseudotype particles expressing HCV E1 and E2 glycoproteins, as well as the infectious chimeric genotype 1a and genotype 2a viruses. Alanine substitutions of residues within a region of E2 involved in binding to CD81 showed that critical E2 contact ...
Protein Tyrosine Nitration
... spleen, is heat and trypsin labile and is induced by endotoxin The products of the reaction are not known but it does not appear to be aminotyrosine ...
... spleen, is heat and trypsin labile and is induced by endotoxin The products of the reaction are not known but it does not appear to be aminotyrosine ...
keyhole limpet haemocyanin, KLH
... staining of tissues from some plant species. Passing serum through HiTrap™ NHS-activated column or a column with CNBr-activated Sepharose™ 4B coupled with KLH, will efficiently remove anti-KLH antibodies in a single purification step. Thus it is a flexible and convenient way of processing serum irre ...
... staining of tissues from some plant species. Passing serum through HiTrap™ NHS-activated column or a column with CNBr-activated Sepharose™ 4B coupled with KLH, will efficiently remove anti-KLH antibodies in a single purification step. Thus it is a flexible and convenient way of processing serum irre ...
The P Domain of the P0 Protein of Plasmodium falciparum Protects
... been studied extensively in humans because of their association with systemic lupus erythematosus (SLE), an autoimmune disorder. Approximately 10 to 15% of patients suffering from SLE possess autoantibodies against the conserved carboxyterminal 22 amino acids of the P proteins (4). The reactive doma ...
... been studied extensively in humans because of their association with systemic lupus erythematosus (SLE), an autoimmune disorder. Approximately 10 to 15% of patients suffering from SLE possess autoantibodies against the conserved carboxyterminal 22 amino acids of the P proteins (4). The reactive doma ...
Antigenic structure of foot-and-mouth disease virus
... Thus the proteins have a repeating order e.g. VP2-VP1-VP3 around each " a p e x " . Note the alternate interactions of different regions of each protein e.g. VP2-VP3 and VP3-VP2 (different sequences) along the " e d g e s " of the icosahedral model. Alternate interactions at the interface between pe ...
... Thus the proteins have a repeating order e.g. VP2-VP1-VP3 around each " a p e x " . Note the alternate interactions of different regions of each protein e.g. VP2-VP3 and VP3-VP2 (different sequences) along the " e d g e s " of the icosahedral model. Alternate interactions at the interface between pe ...
chapter 4 antibody structure ii
... combining site and the epitope or antigenic determinant ("lock and key" concept). The combining site itself is made up by both VL and VH, most directly involving the hypervariable regions or CDRs ("complementarity determining regions") of both. A variety of non-covalent forces are involved in this b ...
... combining site and the epitope or antigenic determinant ("lock and key" concept). The combining site itself is made up by both VL and VH, most directly involving the hypervariable regions or CDRs ("complementarity determining regions") of both. A variety of non-covalent forces are involved in this b ...
μMACS™ Epitope Tag Protein Isolation Kits
... ▲ The lysis of the cells and the magnetic labeling should be performed as described in 2.3, Lysis of cells and 2.4, Magnetic labeling and separation for subsequent analysis by SDS-PAGE. However, neither lysis nor wash buffer should contain SDS since it may impair the biological activity of the immun ...
... ▲ The lysis of the cells and the magnetic labeling should be performed as described in 2.3, Lysis of cells and 2.4, Magnetic labeling and separation for subsequent analysis by SDS-PAGE. However, neither lysis nor wash buffer should contain SDS since it may impair the biological activity of the immun ...
Part 2
... 2. Immunoblotting: This process, also known as Western blotting, is a commonly used analytical technique for detection of specific proteins in a given mixture by means of specific antibodies to the given target protein. a) Electrophoresis: Electrophoresis is a gel-based analytical technique that is ...
... 2. Immunoblotting: This process, also known as Western blotting, is a commonly used analytical technique for detection of specific proteins in a given mixture by means of specific antibodies to the given target protein. a) Electrophoresis: Electrophoresis is a gel-based analytical technique that is ...