The Cytokeratin Filament-Aggregating Protein Filaggrin Is the Target
... They have been found to be highly specific for the disease (615) and their detection is now widely used as a diagnostic test for RA. Although their role has not yet been defined, they are associated with more active and / or severe forms of RA ( 1316) and they are detected at early stages ( 16) and ...
... They have been found to be highly specific for the disease (615) and their detection is now widely used as a diagnostic test for RA. Although their role has not yet been defined, they are associated with more active and / or severe forms of RA ( 1316) and they are detected at early stages ( 16) and ...
Whole body and tissue protein synthesis in cattle
... samples. This was investigated in m. longissimus dorsi, from which myofibrils were prepared as described by Perry & Zydowo (1959). The values for the specific radioactivity of the myofibrillar protein:specific radioactivity of total muscle were 0.96, 0.80 and 0.76 for the animals nos. 439, 440 and 9 ...
... samples. This was investigated in m. longissimus dorsi, from which myofibrils were prepared as described by Perry & Zydowo (1959). The values for the specific radioactivity of the myofibrillar protein:specific radioactivity of total muscle were 0.96, 0.80 and 0.76 for the animals nos. 439, 440 and 9 ...
Colostrum and the Health of Newborn Kids By Jack Mauldin I have
... language and combine it together. I may or may not have succeeded. Antigen is the medical name for a substance that when introduced into the body, stimulates an antibody (a disease fighter). The substances that can generate antibodies can be from bacterial toxins and bacteria. I will use the word “b ...
... language and combine it together. I may or may not have succeeded. Antigen is the medical name for a substance that when introduced into the body, stimulates an antibody (a disease fighter). The substances that can generate antibodies can be from bacterial toxins and bacteria. I will use the word “b ...
Targeting of specific domains of diphtheria toxin by site
... conformation of this region ia the native protein. The epitopes for two of the anti-peptideantibodies were mapped. The results indicated that even though the antisera were raised to peptides containing 14 amino acids (aa) they were directed predominately against a narrow region within the peptide, c ...
... conformation of this region ia the native protein. The epitopes for two of the anti-peptideantibodies were mapped. The results indicated that even though the antisera were raised to peptides containing 14 amino acids (aa) they were directed predominately against a narrow region within the peptide, c ...
Intranasal immunization of mice with Echinococcus
... optimal presentation, i.e. in several copies in a submicroscopic particle, such as a multimer (micelle or proteasome), a liposome (Morein & Simons 1985), or an immuno stimulating complex (iscom) (Morein et al. 1984). An inadequate immune response is not only irrelevant but may even favor the invadin ...
... optimal presentation, i.e. in several copies in a submicroscopic particle, such as a multimer (micelle or proteasome), a liposome (Morein & Simons 1985), or an immuno stimulating complex (iscom) (Morein et al. 1984). An inadequate immune response is not only irrelevant but may even favor the invadin ...
TD12 Characterization of DnaJ substrate specificity Reference
... Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers substrate to binding cavity of DnaK If this is true, what is the substrate specificity of DnaJ and how does it compare to that of DnaK? Ⅱ. ...
... Model: DnaJ first binds to unfolded protein substrates, and then hands them over to DnaK, triggering ATP hydrolysis as it delivers substrate to binding cavity of DnaK If this is true, what is the substrate specificity of DnaJ and how does it compare to that of DnaK? Ⅱ. ...
ISOLATE II PCR and Gel Kit
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
... PCR primers from reactions are eliminated while small DNA fragments are still bound and purified with high recovery. The cut-off for small DNA fragments can be shifted from <50bp to several hundred base pairs by diluting Binding Buffer CB to remove primer-dimers from target PCR products. A yellow pH ...
Supplemental Methods
... from 10 L of specimen spiked with 500 fmol/uL of corresponding heavy (SIS) peptides and diluted 1/10 (v/v) in PBS/0.03% CHAPS. Custom made, prototype magnetic protein G coated beads (1.0 micron diameter; Invitrogen, Oslo, Norway) were used to capture 1 g/well of specific monoclonal antibodies. Sta ...
... from 10 L of specimen spiked with 500 fmol/uL of corresponding heavy (SIS) peptides and diluted 1/10 (v/v) in PBS/0.03% CHAPS. Custom made, prototype magnetic protein G coated beads (1.0 micron diameter; Invitrogen, Oslo, Norway) were used to capture 1 g/well of specific monoclonal antibodies. Sta ...
Agglutination
... protein A in its outer surface that naturally adsorbs the Fc portion of the antibody ...
... protein A in its outer surface that naturally adsorbs the Fc portion of the antibody ...
Genbiotech Store
... smeared bands can be caused by overloading. This is frequently observed with DNA ladders. Biotium offers a 1 kb ladder that has been optimized for use with GelRed (see related products below). 2. Perform post-staining instead of pre-casting. 3. Pour a lower percentage agarose gel for better resoluti ...
... smeared bands can be caused by overloading. This is frequently observed with DNA ladders. Biotium offers a 1 kb ladder that has been optimized for use with GelRed (see related products below). 2. Perform post-staining instead of pre-casting. 3. Pour a lower percentage agarose gel for better resoluti ...
BIGGER, STRONGER and FASTER!
... The debate over how much protein you need as an athlete has spawned a hot bed of controversy. First of all, let me say this. No literature supports the idea that consuming too much protein is harmful or lethal! It is true that when you are consuming a high protein diet, you require lots of water to ...
... The debate over how much protein you need as an athlete has spawned a hot bed of controversy. First of all, let me say this. No literature supports the idea that consuming too much protein is harmful or lethal! It is true that when you are consuming a high protein diet, you require lots of water to ...
GelRed™ Product Information Sheet
... smeared bands can be caused by overloading. This is frequently observed with DNA ladders. Biotium offers a 1 kb ladder that has been optimized for use with GelRed (see related products below). 2. Perform post-staining instead of pre-casting. 3. Pour a lower percentage agarose gel for better resoluti ...
... smeared bands can be caused by overloading. This is frequently observed with DNA ladders. Biotium offers a 1 kb ladder that has been optimized for use with GelRed (see related products below). 2. Perform post-staining instead of pre-casting. 3. Pour a lower percentage agarose gel for better resoluti ...
Analysis of protein carbonylation
... for determination of complex and large analytes is a challenge, especially if they are in physiological fluids ...
... for determination of complex and large analytes is a challenge, especially if they are in physiological fluids ...
Synthesis and Characterization of Hapten-Protein
... Small molecules such as pesticides, drugs, etc. are usually nonimmunogenic and hence do not elicit an immune response unless coupled with some macromolecules such as proteins. It is, therefore, required to modify these small substances (hapten) for coupling with macromolecules (carrier) so as to mak ...
... Small molecules such as pesticides, drugs, etc. are usually nonimmunogenic and hence do not elicit an immune response unless coupled with some macromolecules such as proteins. It is, therefore, required to modify these small substances (hapten) for coupling with macromolecules (carrier) so as to mak ...
Hydrogen exchange mass spectrometry for the analysis of protein
... sample is by dilution. Typically, a solution of protein in a protiated buffer is diluted with a deuterated buffer that has a deuterium content of 99% or more. Dilutions of 15-fold or greater will produce final deuterium concentrations of >95%. This serves to force the labeling reaction (k2) in one d ...
... sample is by dilution. Typically, a solution of protein in a protiated buffer is diluted with a deuterated buffer that has a deuterium content of 99% or more. Dilutions of 15-fold or greater will produce final deuterium concentrations of >95%. This serves to force the labeling reaction (k2) in one d ...
Monoclonal Antibodies Binding Renal Renin
... not prominently recognized in conventional renin antisera since there is usually little antigenic cross reactivity between human and animal renins. As expected, another mouse monoclonal antibody specific for digoxin (our laboratory code: 26-10) did not bind renin in this assay. Measurement of the bi ...
... not prominently recognized in conventional renin antisera since there is usually little antigenic cross reactivity between human and animal renins. As expected, another mouse monoclonal antibody specific for digoxin (our laboratory code: 26-10) did not bind renin in this assay. Measurement of the bi ...
Expressway™ Cell-Free E. coli Expression System
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
... to allow high-level synthesis of your recombinant protein of interest. At one or several time points after initiating the protein synthesis reaction, the reaction is supplemented with an optimized Feed Buffer containing a proprietary mixture of salts, amino acids, and other substrates that are deple ...
... analyzed on sodium dodecyl sulfate (SDS)–10% polyacrylamide gels. SDS-polyacrylamide gel electrophoresis revealed fragments of the expected sizes with no breakdown products (data not shown). The protein concentration was determined with a protein assay kit (Bio-Rad, Hercules, CA) with bovine serum a ...
REVIEWS
... reagents has the advantage that there are several isotypes, which can function therapeutically in either an intact form or as fragments. In the intact molecule, the variable region (Fab) binds antigen, whereas the constant region (Fc) determines the biological properties of the immunoglobulin molecu ...
... reagents has the advantage that there are several isotypes, which can function therapeutically in either an intact form or as fragments. In the intact molecule, the variable region (Fab) binds antigen, whereas the constant region (Fc) determines the biological properties of the immunoglobulin molecu ...
Localization of protein-binding sites within families of proteins
... 2B). In particular, for the randomly generated proteinbinding sites, only 6% of the families have a binding site coverage higher than 0.5; on average, 23% of binding site residues obtained by chance are covered by an existing cumulative binding map. How well does the conservation of location of prot ...
... 2B). In particular, for the randomly generated proteinbinding sites, only 6% of the families have a binding site coverage higher than 0.5; on average, 23% of binding site residues obtained by chance are covered by an existing cumulative binding map. How well does the conservation of location of prot ...
Antibody
... An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, termed an antigen.[1][2] Each tip of t ...
... An antibody, also known as an immunoglobulin, is a large Y-shaped protein produced by B-cells that is used by the immune system to identify and neutralize foreign objects such as bacteria and viruses. The antibody recognizes a unique part of the foreign target, termed an antigen.[1][2] Each tip of t ...
HiTrap Heparin HP,1 ml and 5 ml
... The nature of Heparin Sepharose™ High Performance makes it a very versatile tool for the separation of many proteins e.g. DNA binding proteins, growth factors, coagulation protein synthesis factors and steroid receptors. The ligand in Heparin Sepharose High Performance is a naturally occuring sulfat ...
... The nature of Heparin Sepharose™ High Performance makes it a very versatile tool for the separation of many proteins e.g. DNA binding proteins, growth factors, coagulation protein synthesis factors and steroid receptors. The ligand in Heparin Sepharose High Performance is a naturally occuring sulfat ...
Protein Engineering in the Development of
... reversible inverse phase transition to form insoluble β-spiral aggregates (17, 18); and controlled degradation of a hydrogel matrix is possible with the incorporation of protease cleavage sequences ...
... reversible inverse phase transition to form insoluble β-spiral aggregates (17, 18); and controlled degradation of a hydrogel matrix is possible with the incorporation of protease cleavage sequences ...
(9E10): sc-40 - Santa Cruz Biotechnology
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
... c-Myc-, N-Myc- and L-Myc-encoded proteins function in cell proliferation, differentiation and neoplastic disease. Amplification of the c-Myc gene has been found in several types of human tumors including lung, breast and colon carcinomas. The presence of three sequence motifs in the c-Myc COOH termi ...
Preparation of Lentiviral expression construct DNA
... Secondary HRP conjugated antibody Calculate the amount of HRP conjugated antibody needed to perform the desired experiments and make 1:1000 dilution with 1x Binding Buffer B (100 μl/well). TMB substrate solution Take appropriate volume of TMB Substrate (100 μl/well) and warm it up to room temperatur ...
... Secondary HRP conjugated antibody Calculate the amount of HRP conjugated antibody needed to perform the desired experiments and make 1:1000 dilution with 1x Binding Buffer B (100 μl/well). TMB substrate solution Take appropriate volume of TMB Substrate (100 μl/well) and warm it up to room temperatur ...