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Rousselot® Gelatine
Rousselot® Gelatine

New Generation Collagens
New Generation Collagens

... - Formerly triple spiral collagen, because of lipid residue, had a hint of fish smell. Year by year, the manufacturers have learnt to separate this lipid residue which reduced the fishy smell. Now, the smell of native collagen is subjective. Some like it, some do not. However, any odours evaporate v ...
Solution Structure of the Tandem Acyl Carrier Protein Domains from
Solution Structure of the Tandem Acyl Carrier Protein Domains from

... determined by the amino acid sequence (Figure 5A). However, there is a small portion of the protein which shows a change of +322 Da which suggests that some of the protein may have been modified by the endogenous PPTase from the E. coli expression host. The reaction products show a distribution of m ...
Purification of Arrestin from Bovine Retinas
Purification of Arrestin from Bovine Retinas

Phosphorylation of the F1Fo ATP Synthase Я Subunit
Phosphorylation of the F1Fo ATP Synthase Я Subunit

The Protein Cevalently Linked  to  the  5'... of  Poliovirus RNA by Victor Robert  Ambros
The Protein Cevalently Linked to the 5'... of Poliovirus RNA by Victor Robert Ambros

... be recovered from poliovirus RNA chains varying in length from 7,50C nucleotides (full-sized RNA) to about 500 nucleotides. ...
PDF Full-text
PDF Full-text

Purification and Biochemical Characterization of Digestive Lipase in
Purification and Biochemical Characterization of Digestive Lipase in

b156 - IPB Repository - Bogor Agricultural University
b156 - IPB Repository - Bogor Agricultural University

Marine Biotecnology
Marine Biotecnology

DOCTORAL THESIS
DOCTORAL THESIS

Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad
Biotechnology Explorer™ GMO Investigator™ Kit: A - Bio-Rad

Purification and Characterization of Two Thermostable Proteases
Purification and Characterization of Two Thermostable Proteases

SV 96 Total RNA Isolation System Technical Bulletin
SV 96 Total RNA Isolation System Technical Bulletin

RNA 3`-terminal phosphate cyclases and cyclase
RNA 3`-terminal phosphate cyclases and cyclase

... the 5’-phosphates in RNA and DNA into A5’pp5’N structures. This modification optimally occurs at pH of 5.5-6.0, in contrast with the 3’-phosphate cyclisation reaction, which has optimum at pH 8.0-9.0 and very low activity at pH 5.5/6.0; [26]. In the view of the rather strict requirements for accepta ...
Post-PCR sterilization: a method to control carryover contamination
Post-PCR sterilization: a method to control carryover contamination

Full-Text PDF
Full-Text PDF

Isoenzymes in Clinical Diagnosis
Isoenzymes in Clinical Diagnosis

Purification and Physico-Chemical Analysis of Fractions from the
Purification and Physico-Chemical Analysis of Fractions from the

Purification and Some: Characteristics of a Monomeric Racemase
Purification and Some: Characteristics of a Monomeric Racemase

Formation of Monoterpenes in Antirrhinum majus
Formation of Monoterpenes in Antirrhinum majus

For immediate release GC introduces MI Varnish with Recaldent
For immediate release GC introduces MI Varnish with Recaldent

... has launched MI Varnish, a topical fluoride varnish containing the patented Recaldent technology. Recaldent, also known as casein phosphopeptide – amorphous calcium phosphate (CPP-ACP), is a milk-derived protein that remineralises tooth surfaces by offering bio-available calcium and phosphate ions. ...
Comparing the Prevalence of Verotoxin
Comparing the Prevalence of Verotoxin

... then autoclaved at 21 psi for 15 minutes at 121°C. Once the beaker was ≈ 50°C I poured the agar into sterile Petri dishes. I then waited for the agar to solidify and placed them in the refrigerator upside down for storage until use. The same procedures and concentrations were used for the MacConkey ...
RIBOZYMES
RIBOZYMES

... and III.  Hammerhead ribozymes can be classified into three types based on which helix the 5' and 3' ends are found in.  If the 5' and 3' ends of the sequence contribute to stem I then it is a type I hammerhead ribozyme, and if the and 3' ends of the sequence contribute to stem III then it is a ty ...
TRIzol Reagent
TRIzol Reagent

1 2 3 4 5 ... 69 >

Gel electrophoresis



Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.
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