Amino Acids, Peptides, and Proteins
... 13. The Isoelectric Point of Histones Histones are proteins found in eukaryotic cell nuclei, tightly bound to DNA, which has many phosphate groups. The pI of histones is very high, about 10.8. What amino acid residues must be present in relatively large numbers in histones? In what way do these resi ...
... 13. The Isoelectric Point of Histones Histones are proteins found in eukaryotic cell nuclei, tightly bound to DNA, which has many phosphate groups. The pI of histones is very high, about 10.8. What amino acid residues must be present in relatively large numbers in histones? In what way do these resi ...
Biology - Kenyon College
... IPG strip (pH 4 to 7). 2-D gels were performed on the ESA Investigator 2-D electrophoresis system (Genomic Solutions). Thirteen to 15 ml of each culture was chilled, pelleted, and washed with LBK broth. The cell pellets were then treated with urea-sodium dodecyl sulfate sample buffer and DNase or RN ...
... IPG strip (pH 4 to 7). 2-D gels were performed on the ESA Investigator 2-D electrophoresis system (Genomic Solutions). Thirteen to 15 ml of each culture was chilled, pelleted, and washed with LBK broth. The cell pellets were then treated with urea-sodium dodecyl sulfate sample buffer and DNase or RN ...
Cress and Potato Soluble Epoxide Hydrolases
... substrate have retention times of 12.3, 16.2, and 18.3 min, respectively. Racemic 3H-labeled juvenile hormone-III (23; JH-III), 14Clabeled cis-9,10-epoxystearic acid (24; ESA), and [ 14C]cis-9,10-epoxy12-octadecenoate methyl ester (25; EODM) EH activities were measured as described (33, 35). Electro ...
... substrate have retention times of 12.3, 16.2, and 18.3 min, respectively. Racemic 3H-labeled juvenile hormone-III (23; JH-III), 14Clabeled cis-9,10-epoxystearic acid (24; ESA), and [ 14C]cis-9,10-epoxy12-octadecenoate methyl ester (25; EODM) EH activities were measured as described (33, 35). Electro ...
In vitro gastrointestinal digestion study of a novel bio-tofu
... these findings mentioned above, the novel food structures with improved properties such as controlled energy intake, good satiety and digestibility may develop rapidly in the future. Thus, enzymatic modification of proteins by MTGase could lead to firmer matrices that are digested to a lower extent ...
... these findings mentioned above, the novel food structures with improved properties such as controlled energy intake, good satiety and digestibility may develop rapidly in the future. Thus, enzymatic modification of proteins by MTGase could lead to firmer matrices that are digested to a lower extent ...
DNA Structure Changes Coupled to Protein Binding
... DNA molecule in which bends arise only from thermal agitation remain straight on the average, while intrinsically bent DNA sequences produce nonlinear structures with a preferred direction of bending. Induced DNA bending is usually associated with protein binding, but can also be caused by binding o ...
... DNA molecule in which bends arise only from thermal agitation remain straight on the average, while intrinsically bent DNA sequences produce nonlinear structures with a preferred direction of bending. Induced DNA bending is usually associated with protein binding, but can also be caused by binding o ...
Southern molecular hybridization experiments with parallel
... parallel complementary probes Encouraged by efficient hybridization of Southern blots containing cloned sequences with parallel complementary probes, we attempted to hybridize the probes with genomic Southern blots in 2x SSC solution, as well as in solutions containing 3 M tetramethylammonium chlori ...
... parallel complementary probes Encouraged by efficient hybridization of Southern blots containing cloned sequences with parallel complementary probes, we attempted to hybridize the probes with genomic Southern blots in 2x SSC solution, as well as in solutions containing 3 M tetramethylammonium chlori ...
Metabolic adaptation of Mycobacterium avium subsp
... environment in their natural hosts is limited. Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a chronic and incurable granulomatous enteritis of ruminants, and has been suggested to be a putative aetiological agent of Crohn’s disease in humans. Using a comprehensive LC-MS- ...
... environment in their natural hosts is limited. Mycobacterium avium subsp. paratuberculosis (MAP) causes Johne’s disease, a chronic and incurable granulomatous enteritis of ruminants, and has been suggested to be a putative aetiological agent of Crohn’s disease in humans. Using a comprehensive LC-MS- ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.