IS1245 restriction fragment length polymorphism typing - HAL
... use of this enzyme is the appearance of faint bands in the RFLP patterns (5, 18). This can largely be overcome by using a probe for hybridization prepared by PCR amplification on an IS1245 DNA-containing plasmid and higher-stringency washing conditions after hybridization. Except for the strains wit ...
... use of this enzyme is the appearance of faint bands in the RFLP patterns (5, 18). This can largely be overcome by using a probe for hybridization prepared by PCR amplification on an IS1245 DNA-containing plasmid and higher-stringency washing conditions after hybridization. Except for the strains wit ...
NAME: AKALABU, MAUREEN CHIDINMA COURSE: BCH 301 MAT
... discarded, while the two flanking RNA pieces (called exons) are ligated together. This reaction is called splicing. Besides ribozyme-mediated splicing, which involves RNA alone; there are some splicing reactions that involve RNA-protein complexes. These complexes are called small nucleus ribonucleop ...
... discarded, while the two flanking RNA pieces (called exons) are ligated together. This reaction is called splicing. Besides ribozyme-mediated splicing, which involves RNA alone; there are some splicing reactions that involve RNA-protein complexes. These complexes are called small nucleus ribonucleop ...
HiTrap Chelating HP 1 ml and 5 ml
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
... coupled to the Sepharose High Performance matrix by stable ether bonds via a seven-atom spacer arm. This gives a very stable adsorbent that can be used over the pH range 4–12. When charged with a suitable metal ion, HiTrap Chelating HP will selectively retain proteins if complex-forming amino acid r ...
Expedient synthesis of 1,2,4-triazolin-3
... Lomaiviticin A was reported to cleave DNA under reducing conditions17. Many research programmes have been directed towards understanding the mechanism by which the kinamycins/lomaiviticins cleave DNA and subsequently harnessing this information toward the design of simple analogues for the developme ...
... Lomaiviticin A was reported to cleave DNA under reducing conditions17. Many research programmes have been directed towards understanding the mechanism by which the kinamycins/lomaiviticins cleave DNA and subsequently harnessing this information toward the design of simple analogues for the developme ...
Variation in Glutenin Protein Subunits of Wheat
... them-i, I, m and n-are controlled by a gene or genes on chromosome lB. It would seem likely that the remaining five patterns in this group also are controlled by genes on chromosome IB, but direct evidence for this has not yet been obtained. Three further points may be noted concerning the different ...
... them-i, I, m and n-are controlled by a gene or genes on chromosome lB. It would seem likely that the remaining five patterns in this group also are controlled by genes on chromosome IB, but direct evidence for this has not yet been obtained. Three further points may be noted concerning the different ...
Platinum DNA polymerases
... Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficult to determine fidelity values greater than 100x Taq in a statis ...
... Polymerase fidelity was measured by next-generation sequencing. The background level of experimental errors was estimated from PCR-free library sequencing data. The polymerase fidelities were normalized to Taq polymerase. It is difficult to determine fidelity values greater than 100x Taq in a statis ...
Comparison of methods for high quantity and quality - Funpec-RP
... ABSTRACT. Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three ...
... ABSTRACT. Isolation of sufficient quantities of high quality DNA is a prerequisite for molecular studies. Milk somatic cells can be used; however, inhibitors such as fats and proteins make milk a difficult medium for extracting large amounts of quality DNA. We optimized, evaluated and compared three ...
RNA Biology: Structures to the people! | eLife
... the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. ...
... the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited. ...
Protocol
... DMSO solution in TE (10 mM Tris-HCl, 1 mM EDTA, pH 7.5 in DEPC treated water). For example, add 50 μL StrandBrite™ Green to 10 mL TE buffer to prepare enough working solution to assay 100 samples in a 200 L final volume. Protect the working solution from light by covering it with foil or placing it ...
... DMSO solution in TE (10 mM Tris-HCl, 1 mM EDTA, pH 7.5 in DEPC treated water). For example, add 50 μL StrandBrite™ Green to 10 mL TE buffer to prepare enough working solution to assay 100 samples in a 200 L final volume. Protect the working solution from light by covering it with foil or placing it ...
Discriminating E. coli Isolated from Various Human and Nonhuman
... (sewage, feces, blood and urine) can be further separated into 3 clusters on the MANOVA plot. Ten sewage isolates not in data base were grouped in HM cluster on MONOVA plot. Seven of the 10 sewage isolates were identified as human isolates based on maximum similarity. ...
... (sewage, feces, blood and urine) can be further separated into 3 clusters on the MANOVA plot. Ten sewage isolates not in data base were grouped in HM cluster on MONOVA plot. Seven of the 10 sewage isolates were identified as human isolates based on maximum similarity. ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.