Acetate kinase from CIostridiurn acetobutylicurn : a highly specific
... and applied to affinity chromatography on Procion Blue HE-3B. The enzyme was eluted with ATP and no butyrate kinase activity was detected in the pooled fractions. After column chromatography on hydroxylapatite the acetate kinase seemed to be homogeneous as only one protein band was observed in denat ...
... and applied to affinity chromatography on Procion Blue HE-3B. The enzyme was eluted with ATP and no butyrate kinase activity was detected in the pooled fractions. After column chromatography on hydroxylapatite the acetate kinase seemed to be homogeneous as only one protein band was observed in denat ...
PowerCut™ Dicer
... patent or in violation of any law or regulation. It is the user’s responsibility to determine for himself or herself the suitability of any material and/or procedure for a specific purpose and to adopt such safety precautions as may be necessary. Disclaimer The use of this product in some applicatio ...
... patent or in violation of any law or regulation. It is the user’s responsibility to determine for himself or herself the suitability of any material and/or procedure for a specific purpose and to adopt such safety precautions as may be necessary. Disclaimer The use of this product in some applicatio ...
Definitions of terms relating to the structure and
... Note 1: A gel has a finite, usually rather small, yield stress. Note 2: A gel can contain (i) a covalent polymer network, e.g., a network formed by crosslinking polymer chains or by nonlinear polymerization; (ii) a polymer network formed through the physical aggregation of polymer chains, caused by ...
... Note 1: A gel has a finite, usually rather small, yield stress. Note 2: A gel can contain (i) a covalent polymer network, e.g., a network formed by crosslinking polymer chains or by nonlinear polymerization; (ii) a polymer network formed through the physical aggregation of polymer chains, caused by ...
Di-(2-ethylhexyl) phthalate mediates glycolysis and the TCA cycle
... then washed with electrophoresis buffer (25 mM Tris, 192 mM glycine, 0.1 % SDS) and placed on a 12 % polyacrylamide gel containing 0.1 % SDS. The gels were sealed with low melting point agarose, and the second dimension of gel electrophoresis was conducted at 70 v for 30 min and then 130 v for appro ...
... then washed with electrophoresis buffer (25 mM Tris, 192 mM glycine, 0.1 % SDS) and placed on a 12 % polyacrylamide gel containing 0.1 % SDS. The gels were sealed with low melting point agarose, and the second dimension of gel electrophoresis was conducted at 70 v for 30 min and then 130 v for appro ...
Redalyc.MOLECULAR CHARACTERIZATION OF CRUDE SEED
... extract; interestingly, in this study a protein of ~30kDa is highlighted, which suggests that it was not possible to break the peptide bonds of protein by the effect of the salt and that it is necessary to improve the method by increasing the stirring time of the solution. Because Moringa seed extra ...
... extract; interestingly, in this study a protein of ~30kDa is highlighted, which suggests that it was not possible to break the peptide bonds of protein by the effect of the salt and that it is necessary to improve the method by increasing the stirring time of the solution. Because Moringa seed extra ...
NZY Ribonuclease Inhibitor
... Use 40 units of protein in a 20 µL reaction mixture to protect the template RNA, improve total cDNA yields and increase the percentage of full length cDNA. The presence of NZY Ribonuclease Inhibitor does not affect the use of RNase H after first-strand cDNA synthesis. ...
... Use 40 units of protein in a 20 µL reaction mixture to protect the template RNA, improve total cDNA yields and increase the percentage of full length cDNA. The presence of NZY Ribonuclease Inhibitor does not affect the use of RNase H after first-strand cDNA synthesis. ...
Biochemistry - Stryer - Science and Technology
... to separate protein molecules (Section 3.1). Because the phosphodiester backbone of DNA is highly negatively charged, this technique is also suitable for the separation of nucleic acid fragments. For most gels, the shorter the DNA fragment, the farther the migration. Polyacrylamide gels are used to ...
... to separate protein molecules (Section 3.1). Because the phosphodiester backbone of DNA is highly negatively charged, this technique is also suitable for the separation of nucleic acid fragments. For most gels, the shorter the DNA fragment, the farther the migration. Polyacrylamide gels are used to ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.