RevertAid First Strand cDNA Synthesis Kit, #K1621
... bands should be visible after denaturing agarose gel electrophoresis of total eukaryotic RNA. Follow general recommendations to avoid RNase contamination. Low template purity. Trace amounts of agents used in RNA purification protocols may remain in solution and inhibit first strand synthesis, e.g., ...
... bands should be visible after denaturing agarose gel electrophoresis of total eukaryotic RNA. Follow general recommendations to avoid RNase contamination. Low template purity. Trace amounts of agents used in RNA purification protocols may remain in solution and inhibit first strand synthesis, e.g., ...
Molecular diagnosis of human immuno deficiency virus (HIV) by
... real- time PCR for detection of specific nucleic acid and viral genome respectively. Viral genome was extracted and reverse transcription was performed with M-Mulv and the cDNA kept at -80º C. The semi nested PCR products were analyzed by running on an agarose gel electrophoresis. Fifteen samples we ...
... real- time PCR for detection of specific nucleic acid and viral genome respectively. Viral genome was extracted and reverse transcription was performed with M-Mulv and the cDNA kept at -80º C. The semi nested PCR products were analyzed by running on an agarose gel electrophoresis. Fifteen samples we ...
p19 siRNA Binding Protein | NEB
... 3. p19 siRNA Binding Protein does not bind ssRNA.Double-stranded 21 bp RNA with a 2-base overhang and a 5´ phosphate binds the most efficiently. Double-stranded miRNAs with mismatched base pairs will bind with lower affinity. 4. Due to the difference in molecular weight between p19 siRNA Binding Pro ...
... 3. p19 siRNA Binding Protein does not bind ssRNA.Double-stranded 21 bp RNA with a 2-base overhang and a 5´ phosphate binds the most efficiently. Double-stranded miRNAs with mismatched base pairs will bind with lower affinity. 4. Due to the difference in molecular weight between p19 siRNA Binding Pro ...
A Human Centromere Protein, CENP-B, Has a DNA Binding Domain
... and thrombin from human plasma from human placenta (Boehringer Mannheim GmbN). GIu-C protease hydrolyzes specifically peptide bonds at the carboxylic side of glutamic acid. Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving aromatic L-amino acids (Tyr, Phe, and Trp). Thr ...
... and thrombin from human plasma from human placenta (Boehringer Mannheim GmbN). GIu-C protease hydrolyzes specifically peptide bonds at the carboxylic side of glutamic acid. Chymotrypsin preferentially catalyzes the hydrolysis of peptide bonds involving aromatic L-amino acids (Tyr, Phe, and Trp). Thr ...
Probing the active site of homoserine trans
... which could not be detected in the wild-type strain, not carrying the metA plasmid. These spots corresponded to HTS, as could be determined by Western blotting with anti-HTS antibodies and mass spectrometry. The four spots had a similar apparent molecular weight and differed in their pI (Fig. 1). Suc ...
... which could not be detected in the wild-type strain, not carrying the metA plasmid. These spots corresponded to HTS, as could be determined by Western blotting with anti-HTS antibodies and mass spectrometry. The four spots had a similar apparent molecular weight and differed in their pI (Fig. 1). Suc ...
AccuStart™ II Mouse Genotyping Kit
... The AccuStart II Mouse Genotyping Kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with ...
... The AccuStart II Mouse Genotyping Kit is designed for fast and easy preparation of PCR-ready DNA extracts and endpoint PCR analysis from tissues such as tail snips and ear punches commonly used for genotyping knockout and transgenic animals. The kit combines Extracta™ DNA Prep for PCR - Tissue with ...
Replacing Traditional SDS-PAGE and Bradford Techniques
... Figure 2B displays the analysis of the corresponding fractions with SDS-PAGE, stained with Coomassie. Whereas the SDS-PAGE analysis of the samples required two gels, the 5100 ALP is capable of analyzing up to twelve 96-well plates in one “job”. Therefore, samples can be compared more easily and view ...
... Figure 2B displays the analysis of the corresponding fractions with SDS-PAGE, stained with Coomassie. Whereas the SDS-PAGE analysis of the samples required two gels, the 5100 ALP is capable of analyzing up to twelve 96-well plates in one “job”. Therefore, samples can be compared more easily and view ...
Chapter 3 Kinetic analysis of ribozyme cleavage
... group II introns are changed during single-turnover reactions. However, multipleturnover enzymes can be engineered. An early example was the L-19 version of the Tetrahymena group I intron by Zaug and Cech (5). The first 19 nt of the ribozyme were removed, leaving the G-rich 3 -strand of the P1 stem– ...
... group II introns are changed during single-turnover reactions. However, multipleturnover enzymes can be engineered. An early example was the L-19 version of the Tetrahymena group I intron by Zaug and Cech (5). The first 19 nt of the ribozyme were removed, leaving the G-rich 3 -strand of the P1 stem– ...
Title Detection of osteopontin as matrix protein in calcium
... immediately before application to individual wells. A 10% gel plate was put into an electrophoresis tank filled with electrolyte buffer (15.1 g/I, Tris, 72 g/I ...
... immediately before application to individual wells. A 10% gel plate was put into an electrophoresis tank filled with electrolyte buffer (15.1 g/I, Tris, 72 g/I ...
pharmaceutical biochemistry
... skeletal muscle. Mobilization of energy from glycogen stores is a rapid process and it works under anaerobic conditions as well, however it contains less energy than the same amount of stored fat. Glycogen is the polymeric form of glucose using 1,4-glycosidic bonds inside the chain and 1,6-bonds in ...
... skeletal muscle. Mobilization of energy from glycogen stores is a rapid process and it works under anaerobic conditions as well, however it contains less energy than the same amount of stored fat. Glycogen is the polymeric form of glucose using 1,4-glycosidic bonds inside the chain and 1,6-bonds in ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.