DNase I (AMPD1) - Technical Bulletin - Sigma
... reactions should be run without adding reverse transcriptase to check for amplification of contaminating DNA. These precautions are especially recommended if PCR primers do not span an intron, if pseudogenes that lack the intron may be present in the target cells or tissue,1 or if the RNA will be us ...
... reactions should be run without adding reverse transcriptase to check for amplification of contaminating DNA. These precautions are especially recommended if PCR primers do not span an intron, if pseudogenes that lack the intron may be present in the target cells or tissue,1 or if the RNA will be us ...
SPRI_buffers_v2_2
... Chelating agents EDTA and citrate may interfere with some enzymatic reactions by sequestering divalent cations such as Mg2+ and Mn2+. These ions may damage nucleic acids or activate contaminating nucleases. On the other hand, sequestering these ions may interfere with downstream reactions that requi ...
... Chelating agents EDTA and citrate may interfere with some enzymatic reactions by sequestering divalent cations such as Mg2+ and Mn2+. These ions may damage nucleic acids or activate contaminating nucleases. On the other hand, sequestering these ions may interfere with downstream reactions that requi ...
Section 2.3 Carbon
... Nucleic Acids • The chemical basis of life is a Unifying Theme for all organisms ...
... Nucleic Acids • The chemical basis of life is a Unifying Theme for all organisms ...
Coverage Strategy for Compounds
... Included routinely in topical compounds. Bulk powder forms of these drug chemicals have not been adequately studied for transdermal administration. Efficacy and safety are unknown. FDA approved drugs for transdermal administration are available for some drugs in these therapeutic classes. Hormone re ...
... Included routinely in topical compounds. Bulk powder forms of these drug chemicals have not been adequately studied for transdermal administration. Efficacy and safety are unknown. FDA approved drugs for transdermal administration are available for some drugs in these therapeutic classes. Hormone re ...
Multistep Small-Molecule Synthesis Programmed by
... iterated cycles of translation, selection, amplification, and diversification, collectively referred to as molecular evolution. Although the power of Nature’s approach is well appreciated,1 we cannot apply molecular evolution to molecules other than proteins or nucleic acids because we lack a method ...
... iterated cycles of translation, selection, amplification, and diversification, collectively referred to as molecular evolution. Although the power of Nature’s approach is well appreciated,1 we cannot apply molecular evolution to molecules other than proteins or nucleic acids because we lack a method ...
Identification of Virgibacillus species using 16S rRNA gene Sequence
... described below. The DNA fragment was excised from the agarose gel with a clean sharp scalpel. Then the gel slice was weighed in an eppendorf. We then added 3 volumes of buffer QG to 1 volume of gel (100 mg ~ 100 µl). The mixture was then incubated at 500C for 10 minutes. The gel was dissolved by vo ...
... described below. The DNA fragment was excised from the agarose gel with a clean sharp scalpel. Then the gel slice was weighed in an eppendorf. We then added 3 volumes of buffer QG to 1 volume of gel (100 mg ~ 100 µl). The mixture was then incubated at 500C for 10 minutes. The gel was dissolved by vo ...
3.3 How Do You Identify and Clone a Gene of Interest?
... 3.4 What Can You Do with a Cloned Gene? Applications of Recombinant DNA Technology • Agarose Gel Electrophoresis – To run a gel, it is submerged in a buffer solution that conducts electricity – DNA is loaded into wells at the top of the gel – Electric current is applied to the ends of the gel • DNA ...
... 3.4 What Can You Do with a Cloned Gene? Applications of Recombinant DNA Technology • Agarose Gel Electrophoresis – To run a gel, it is submerged in a buffer solution that conducts electricity – DNA is loaded into wells at the top of the gel – Electric current is applied to the ends of the gel • DNA ...
Production and Purification of Recombinant Fluorescent Protein
... demonstrated to be no less viable than corresponding wild type cells . In live cells, fluorescent proteins are most commonly employed to track the localization and dynamics of proteins, organelles, and other cellular compartments. A variety of techniques have been developed to construct fluorescent ...
... demonstrated to be no less viable than corresponding wild type cells . In live cells, fluorescent proteins are most commonly employed to track the localization and dynamics of proteins, organelles, and other cellular compartments. A variety of techniques have been developed to construct fluorescent ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.