The GPI Transamidase Complex of Saccharomyces
... accumulation of the complete GPI lipid CP2 and of unprocessed GPI precursor proteins. Gpi8p and Gpi16p are unstable if either of them is removed by depletion. Similarly, when Gpi8p is overexpressed, it largely remains outside the 430 – 650-kDa transamidase complex and is unstable. Overexpression of ...
... accumulation of the complete GPI lipid CP2 and of unprocessed GPI precursor proteins. Gpi8p and Gpi16p are unstable if either of them is removed by depletion. Similarly, when Gpi8p is overexpressed, it largely remains outside the 430 – 650-kDa transamidase complex and is unstable. Overexpression of ...
Table of Contents - Milan Area Schools
... Carrier proteins in the cell surface membrane allow substances to enter the cell. Chemical signals such as hormones bind to proteins on the cell surface membrane. ...
... Carrier proteins in the cell surface membrane allow substances to enter the cell. Chemical signals such as hormones bind to proteins on the cell surface membrane. ...
Phenylalanineaminopeptidase of L. pneumophila
... presence of one protein band, RF0.6 (relative to the marker dye mobility), possessing activity against L-Phe-pNA and L-Leu-pNA, after PAGE at pH 8.3. SDS-PAGE also revealed only one protein band corresponding to a molecular weight of 35000. The same value was found for the native enzyme by gel filtr ...
... presence of one protein band, RF0.6 (relative to the marker dye mobility), possessing activity against L-Phe-pNA and L-Leu-pNA, after PAGE at pH 8.3. SDS-PAGE also revealed only one protein band corresponding to a molecular weight of 35000. The same value was found for the native enzyme by gel filtr ...
2.3 Carbon-Based Molecules
... • Proteins differ in the number and order of amino acids. – Amino acids interact to give a protein its shape. ...
... • Proteins differ in the number and order of amino acids. – Amino acids interact to give a protein its shape. ...
Nick Translation DNA Labeling Systems
... of modified nucleotide analogs in the synthetic reactions results in chemically modified DNA. In one hour to overnight reactions, newly synthesized labeled DNA is generated in high yield. Random primed labeling reactions are optimized for generating maximum amounts of labeled DNA from 50 ng to 1 µg ...
... of modified nucleotide analogs in the synthetic reactions results in chemically modified DNA. In one hour to overnight reactions, newly synthesized labeled DNA is generated in high yield. Random primed labeling reactions are optimized for generating maximum amounts of labeled DNA from 50 ng to 1 µg ...
Adenovirus-associated Virus Structural Protein Sequence Homology
... 0.2 mg/ml. The mixture was agitated for 5 min at room temperature, whereupon the reaction was terminated by the addition of 25/tg of sodium metabisulphite dissolved in Io/tl of buffer I. Electrophoresis of proteins. Iodinated virion proteins were dialysed exhaustively against o.ol M-phosphate buffer ...
... 0.2 mg/ml. The mixture was agitated for 5 min at room temperature, whereupon the reaction was terminated by the addition of 25/tg of sodium metabisulphite dissolved in Io/tl of buffer I. Electrophoresis of proteins. Iodinated virion proteins were dialysed exhaustively against o.ol M-phosphate buffer ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.