Protocol for archaeal 16S (A16S) rRNA amplification and
... of PCR uses locus-‐specific primers with overhang adapters (A2F_Nex and 519R_Nex). The locus-‐ specific region of the forward primer was based on the A2F primer from Reysenbach et al.(1995), which is specifi ...
... of PCR uses locus-‐specific primers with overhang adapters (A2F_Nex and 519R_Nex). The locus-‐ specific region of the forward primer was based on the A2F primer from Reysenbach et al.(1995), which is specifi ...
Bacterial Screening PCR Kit
... Options for Preparation of Bacterial DNA [Option 1] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Wash the pellet containing the separated bacteria (see step 2 in section C above) using sterilized water and TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) then suspend in a 200 μ l ...
... Options for Preparation of Bacterial DNA [Option 1] (Use of 1.5 ml micro test tube with screw cap is recommended.) 1) Wash the pellet containing the separated bacteria (see step 2 in section C above) using sterilized water and TE buffer (10 mM Tris-HCl, 0.1 mM EDTA, pH 8.0) then suspend in a 200 μ l ...
Evaluation of Genotypic variation using SDS-PAGE
... described by (Jha and Ohri, 2002) [5]. The seed material was homogenized using 0.1 M TrisHCl buffer (pH: 7.5) in the ratio 1:10 (W/V). Total protein was extracted after centrifugation at 17,600 g for 20 min at 4 °C and clear supernatants were used for analysis. ...
... described by (Jha and Ohri, 2002) [5]. The seed material was homogenized using 0.1 M TrisHCl buffer (pH: 7.5) in the ratio 1:10 (W/V). Total protein was extracted after centrifugation at 17,600 g for 20 min at 4 °C and clear supernatants were used for analysis. ...
Advanced Organic Chemistry of Nucleic Acids
... of the primary structure of nucleic acids, their synthesis, macromolecular structure, and chemical modification. Many chapters, including all of Chapter 10 dealing with ribozymes, have entirely been written anew. However, at the same time (much to our surprise), many fundamentals of nucleic acid che ...
... of the primary structure of nucleic acids, their synthesis, macromolecular structure, and chemical modification. Many chapters, including all of Chapter 10 dealing with ribozymes, have entirely been written anew. However, at the same time (much to our surprise), many fundamentals of nucleic acid che ...
protein - Portal UniMAP
... segments line up side by side Each individual segment = β-strand Each β-strand is fully extended β-pleated sheet stabilized by hydrogen bonds form between the polypeptide backbone N-H and carbonyl groups of adjacent chains ...
... segments line up side by side Each individual segment = β-strand Each β-strand is fully extended β-pleated sheet stabilized by hydrogen bonds form between the polypeptide backbone N-H and carbonyl groups of adjacent chains ...
10858_2015_9967_MOESM1_ESM
... five selected RNA constructs shown in a and b, where lanes marked with D correspond to transcriptions performed in 20% of DMSO. The loading mixture contained 50% formamide, 10% of the transcription mixture for a and 1% of the transcription mixture for b (0.2% DMSO), respectively. Gels were stained w ...
... five selected RNA constructs shown in a and b, where lanes marked with D correspond to transcriptions performed in 20% of DMSO. The loading mixture contained 50% formamide, 10% of the transcription mixture for a and 1% of the transcription mixture for b (0.2% DMSO), respectively. Gels were stained w ...
Comparative analyses of Saccharomyces cerevisiae RNAs using
... areas of a yeast colony for subsequent Northern blot analyses with speci¢c probes could be of considerable interest. 3.2. RNA Nano Assay makes it possible to analyse RNAs protected against RNases by formamide When comparative studies on the content of particular mRNAs within a total yeast RNA are pe ...
... areas of a yeast colony for subsequent Northern blot analyses with speci¢c probes could be of considerable interest. 3.2. RNA Nano Assay makes it possible to analyse RNAs protected against RNases by formamide When comparative studies on the content of particular mRNAs within a total yeast RNA are pe ...
Biotechnology toolkit part 2
... containing millions of strands of DNA of identical length. A dye is added to the DNA sample, which moves slightly faster than the shortest fragments so you can see how far the bands have travelled. The DNA can be visualised by adding ethidium bromide, which fluoresces under UV light. Gel electrophor ...
... containing millions of strands of DNA of identical length. A dye is added to the DNA sample, which moves slightly faster than the shortest fragments so you can see how far the bands have travelled. The DNA can be visualised by adding ethidium bromide, which fluoresces under UV light. Gel electrophor ...
Chapter 3 Amino Acids, Peptides, Proteins
... molecule. Another way to think is that charge-charge interaction stabilizes the zwitterion so is occurs more easily Use a different logic on NH2. Base is close to COO-. These electronegative atoms (O’s of COO-) pull electrons toward them. NH3+ is more easily deprotonated (more acidic) because these ...
... molecule. Another way to think is that charge-charge interaction stabilizes the zwitterion so is occurs more easily Use a different logic on NH2. Base is close to COO-. These electronegative atoms (O’s of COO-) pull electrons toward them. NH3+ is more easily deprotonated (more acidic) because these ...
19-9-ET-V1-S1__preci..
... the salt has to be added in small amount under constant stirring to avoid accumulation of high concentration of salts. When large amount of salt is added to an aqueous solution of proteins the salt requires more amount of water for its dissolution. This leads to competition for water molecule on the ...
... the salt has to be added in small amount under constant stirring to avoid accumulation of high concentration of salts. When large amount of salt is added to an aqueous solution of proteins the salt requires more amount of water for its dissolution. This leads to competition for water molecule on the ...
Methods S1.
... mitochondrial respiration was initiated using glutamate (20mM, Aldrich, Steinheim, Germany) with malate (20mM, Aldrich, Steinheim, Germany). Exactly 60 s later, state 3 respiration was initiated by 200 μmol/L ADP injected into the respiration chamber. Respiration rates were recorded under state 3 co ...
... mitochondrial respiration was initiated using glutamate (20mM, Aldrich, Steinheim, Germany) with malate (20mM, Aldrich, Steinheim, Germany). Exactly 60 s later, state 3 respiration was initiated by 200 μmol/L ADP injected into the respiration chamber. Respiration rates were recorded under state 3 co ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.