RNA/Protein Purification 96-Well Kit
... from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. It is often necessary to isolate total RNA and proteins from a single sample, such as for studies of gene expression including gene silencing experiments, mRNA knockdowns or experiments corr ...
... from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. It is often necessary to isolate total RNA and proteins from a single sample, such as for studies of gene expression including gene silencing experiments, mRNA knockdowns or experiments corr ...
Protein Phosphorylation in Rhodomicrobium vmnielii
... Two-dimensional electrophoresis was done using a non-denaturing gel in the first dimension, adapted by D. Porter (personal communication) from a system described by Sargent & George (1975), and by SDS-PAGE in the second dimension. Samples were analysed in the first dimension by electrophoresis on a ...
... Two-dimensional electrophoresis was done using a non-denaturing gel in the first dimension, adapted by D. Porter (personal communication) from a system described by Sargent & George (1975), and by SDS-PAGE in the second dimension. Samples were analysed in the first dimension by electrophoresis on a ...
PowerPoint file - Birmingham
... • slow growth and increased cell size phenotypes • UV sensitivity • increased recombination rates ...
... • slow growth and increased cell size phenotypes • UV sensitivity • increased recombination rates ...
Lehninger Principles of Biochemistry 5/e
... The net charge is zero; such ions are called Zwitterions At alkaline pH, the amino group is neutral –NH2 and the amino acid is in the anionic form. The R groups also gets protonated. This varies from amino acid to amino acid. Thus different amino acids have different pKa. ...
... The net charge is zero; such ions are called Zwitterions At alkaline pH, the amino group is neutral –NH2 and the amino acid is in the anionic form. The R groups also gets protonated. This varies from amino acid to amino acid. Thus different amino acids have different pKa. ...
Quant-iT™ Assay Kits for microplate
... linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of ...
... linear detection range of 0.2–100 ng and is selective for dsDNA, even in the presence of an equal mass of RNA. The x-axis gives the mass of nucleic acid when DNA or RNA is assayed alone; in the 1:1 mixture, the total mass of nucleic acid is double the amount shown. The inset shows the sensitivity of ...
SDS-PAGE strongly overestimates the molecular
... addition these domains are rather acidic [2,3]. There are several acidic proteins which, probably due to restricted SDS-binding, give too high apparent molecular masses in SDS-PAGE (see, e.g., [13]), although the discrepancies seem not as extreme as observed for H and M. That the higher degree of ph ...
... addition these domains are rather acidic [2,3]. There are several acidic proteins which, probably due to restricted SDS-binding, give too high apparent molecular masses in SDS-PAGE (see, e.g., [13]), although the discrepancies seem not as extreme as observed for H and M. That the higher degree of ph ...
Isolation and Amino Acid Sequence of Two New PR
... Two New PR:4 Proteins from Wheat gradient of 35–55 mM NaCl in the piperazine buffer (150 ml of each); and washing with 75 ml of the piperazine buffer containing 100 mM NaCl. Elution was monitored for absorbance at 280 nm. Wheatwin1 and wheatwin3 were eluted in different peaks. Wheatwin2 and wheatwi ...
... Two New PR:4 Proteins from Wheat gradient of 35–55 mM NaCl in the piperazine buffer (150 ml of each); and washing with 75 ml of the piperazine buffer containing 100 mM NaCl. Elution was monitored for absorbance at 280 nm. Wheatwin1 and wheatwin3 were eluted in different peaks. Wheatwin2 and wheatwi ...
Principles of Chromatography File
... • After five equilibrations, the compound is distributed throughout the whole column but is maximally concentrated at the center of the column. • If the distribution coefficient is <1 • More than 50% of the compound would be left on solid phase after each equilibration and the concentration peak is ...
... • After five equilibrations, the compound is distributed throughout the whole column but is maximally concentrated at the center of the column. • If the distribution coefficient is <1 • More than 50% of the compound would be left on solid phase after each equilibration and the concentration peak is ...
Nucleic Acid structure - part 1
... Discovery of DNA structure and its role in housing genetic information Avery-MacLeod-McCarty 1944 Direct evidence that DNA carries genetic info ...
... Discovery of DNA structure and its role in housing genetic information Avery-MacLeod-McCarty 1944 Direct evidence that DNA carries genetic info ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.