DP Chemistry Standard Level
... chains of 2-amino acids formed by condensation reactions. • Macromolecule: a really big molecule • Amino acid • Condensation Reactions: This is when two typically larger molecules come together and in the process release a smaller ...
... chains of 2-amino acids formed by condensation reactions. • Macromolecule: a really big molecule • Amino acid • Condensation Reactions: This is when two typically larger molecules come together and in the process release a smaller ...
PPT File
... • DNA samples can be studied and compared by DNA fingerprinting • DNA is digested with restriction enzymes and then run on an agarose gel • When soaked in ethidium bromide, the DNA fragments can be seen directly under UV light • If greater sensitivity needed or if number of fragments would be too gr ...
... • DNA samples can be studied and compared by DNA fingerprinting • DNA is digested with restriction enzymes and then run on an agarose gel • When soaked in ethidium bromide, the DNA fragments can be seen directly under UV light • If greater sensitivity needed or if number of fragments would be too gr ...
Ch. 13 Bioengineering
... • Separating DNA – In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel. – When the power is turned on, the negatively-charged DNA molecules move toward the positive end of the gel. ...
... • Separating DNA – In gel electrophoresis, DNA fragments are placed at one end of a porous gel, and an electric voltage is applied to the gel. – When the power is turned on, the negatively-charged DNA molecules move toward the positive end of the gel. ...
Plasma Proteins - neutralposture
... when subjected to an electric field Positively charged particles (cations) move to cathode ...
... when subjected to an electric field Positively charged particles (cations) move to cathode ...
plasmid isolation from bacteria
... between 1 and 200 kb. Extremely large plasmids with sizes far beyond 200 kb are also known. Plasmids of more than 50 kb might be characterized as “large”, plasmids of less than 10 kb as “small”. The aim of this compilation is to describe some fast methods for small scale plasmid isolation leading to ...
... between 1 and 200 kb. Extremely large plasmids with sizes far beyond 200 kb are also known. Plasmids of more than 50 kb might be characterized as “large”, plasmids of less than 10 kb as “small”. The aim of this compilation is to describe some fast methods for small scale plasmid isolation leading to ...
DNA - JSH BIOLOGY with Ms. Barbanel
... a. DNA can be visualized through the process of electrophoresis b. In the lab, DNA molecules are cut by restriction enzymes into fragments of various sizes. Restriction enzymes cut at specific sequences throughout the DNA. c. The resulting fragments are forced to move along a gel-coated plate under ...
... a. DNA can be visualized through the process of electrophoresis b. In the lab, DNA molecules are cut by restriction enzymes into fragments of various sizes. Restriction enzymes cut at specific sequences throughout the DNA. c. The resulting fragments are forced to move along a gel-coated plate under ...
UV Light Cleaning of Amputee Liners RAKES Inc. NCIIA Grant
... on their amputated limbs. Often times, fungal and bacterial infections develop due to inabilities to completely clean the gel. This is a widespread problem among amputees, especially those who are active athletically, as the sweat from their physical activity produces metabolites that are favorable ...
... on their amputated limbs. Often times, fungal and bacterial infections develop due to inabilities to completely clean the gel. This is a widespread problem among amputees, especially those who are active athletically, as the sweat from their physical activity produces metabolites that are favorable ...
MOLECULAR BIOLOGY and GENETICS
... genetics are also introduced and some of the current techniques used in molecular biology research are presented. The practical course (6 five hour sessions; one every fortnight) gives you hands-on experience in the methodology behind many of the modern techniques in molecular biology. It is hoped t ...
... genetics are also introduced and some of the current techniques used in molecular biology research are presented. The practical course (6 five hour sessions; one every fortnight) gives you hands-on experience in the methodology behind many of the modern techniques in molecular biology. It is hoped t ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.