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BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)
Unit 13 Biochemistry and biochemical techniques
Biological Molecules
Assignment front sheet 13.1
Learner name
Assessor name
Mrs Turner-Smith
Date issued
Completion date
07/09/2015
09/10/2015
Submitted on
Unit number and title
Unit 13: Biochemistry and biochemical techniques
Assignment title
Biological molecules
In this assessment you will have opportunities to provide evidence against the following criteria.
Criteria
reference
To achieve the criteria the evidence must show that the
learner is able to:
Task
No.
Evidence
identify the structure and function of biological molecules
P1
Complete the worksheet showing the structure of carbohydrates and its
function
1
Essay on the properties of water
2
use laboratory separative techniques for the characterisation
of biological molecules
P2
3a
Complete the worksheet on TLC of amino acids
Carry out separation of DNA using gel electrophoresis
3b
interpret data obtained from experiments designed to
separate biological molecules
M1
4
Calculate the Rf values from your TLC worksheet and write a
conclusion
discuss the relationship between structure and function for
carbohydrates and lipids
D1
Complete the worksheet showing the structure of carbohydrates and
their function
1
Essay on the properties of water
2
Complete the worksheet showing the structure of Lipids and their
function
5
Learner declaration
I certify that the work submitted for this assignment is my own and research sources are fully acknowledged.
Learner signature:
Date:
1
Unit 13 Biochemistry and biochemical techniques
BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)
Assignment brief 13.1
Qualification
BTEC Level 3 Subsidiary Diploma in Applied Science (Applied Biology)
Unit number and title
Unit 13: Biochemistry and biochemical techniques
Start date
07/09/2015
Deadline
09/10/2015
Assessor name
Mrs Turner-Smith
Assignment title
Biological molecules
The purpose of this assignment is to be able to investigate properties of biological molecules in living organisms.
Scenario
The lab you work in has a sophisticated computer system used to store important files and information.
Unfortunately a virus has infected the computer system and although no important data were lost, the files have
become disorganised. It is your job to re-organise some of the files, conduct experiments to check that some of the
files are correct and produce some documents for files that cannot be found. (You should identify hazards and
necessary control measures to reduce any risks.)
P1: Identify the structure and function
of biological molecules
Task 1
D1: discuss the relationship between structure
and function for carbohydrates and lipids
Complete the worksheet to demonstrate that you understand the structure and function of carbohydrates. You need
to discuss the components of starch and how it differs from cellulose. The worksheet will also demonstrate the
properties of carbohydrates that make it a good energy storage molecule.
Date Due:
Task 2
This provides evidence for P1 and D1
P1 Identify the structure and function of biological molecules
Following the lessons on the structure and properties of water write an essay explaining its importance in the
maintenance of life. You must include the following:

Specific heat capacity and its importance

Density and its importance

Polarity and its importance

Cohesion and its importance

Adhesion and its importance

Surface tension and its importance
Date Due:
Task 3a
This provides evidence for P1
P2 Use laboratory separative techniques for the characterisation of biological molecules
The computer was also used to file information about the chemicals in the stores. As a result there are now a
number of chemicals that cannot be identified. You should investigate methods used to separate amino acids and
proteins. Provide information about thin layer chromatography, and electrophoresis. This information could be used
by people working in the laboratory to sort out the unknown chemicals in the stores.
For successful completion of this task you need to complete the worksheet on the TLC separation of 2 unknown
amino acids
Date Due:
2
This provides evidence for P2
BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)
Task 3b
Unit 13 Biochemistry and biochemical techniques
Biological Molecules
P2 Use laboratory separative techniques for the characterisation of biological molecules
Using the method of gel electrophoresis separate out a sample of DNA. Finally you need to create a crib sheet
explaining how electrophoresis can be used to separate a DNA sample. You need to explain the technique and
provide a method.
Date Due:
Task 4
This provides evidence for P2
M1 Interpret data obtained from experiments designed to separate biological molecules
Interpret the data from task 2a. You need to identify the unknown amino acids and compare them to published
data, draw up a conclusion of your results. Your interpretation should include discussions about how the structure of
the molecules allows the separation technique to work and a conclusion stating the products of the separations
(from your own data and those provided) and how you can be confident that this is the case, again referring to the
structure of the molecules concerned.
Date Due:
Task 5
This provides evidence for M1
P1 Identify the structure and function of
biological molecules
D1 discuss the relationship between structure
and function for triglycerides and phospholipids
Another important file is missing. The file contained specific information about triglycerides and phospholipids and
how their structure enables them to perform their function.
The files need to be replaced. You should complete the two worksheets explaining where these biological
molecules are found, used and formed. The worksheet will also cover how the structure of lipids makes it a suitable
molecule for its function.
Date Due:
This provides evidence for P1 & D1
Sources of information
Alberts B et al – Essential Cell Biology (Garland Science, 2003) ISBN 9780815334811
Hames D and Hooper N – Instant Notes in Biochemistry (Instant Notes Series) (Bios Scientific Publishers, 2005)
ISBN 9780415367783
Purves W K – Life: The Science of Biology Volume 1 The Cell and Heredity (WH Freeman & Co Ltd, 2001) ISBN
9780716738732
Salway J G – Metabolism at a Glance, 3rd Edition (Blackwell Publishing, 2003) ISBN 9781405107167
Adds J et al – Respiration and Coordination (Nelson Advanced Science: Biology Series)
(Nelson Thornes, 2005) ISBN 9780748774890
Boyle M et al – Human Biology (Collins Advanced Science Series) (Collins Educational, 2002)
ISBN 9780007135998
Johnson M D – Human Biology: Concepts and Current Issues, 3rd Edition (Benjamin
Cummings, 2005) ISBN 9780805372014
Evidence checklist
[Summarise evidence required, e.g. ‘leaflet’, ‘presentation notes’ etc.]
[tick boxes]
Complete the worksheet showing the structure of carbohydrates and its function
Essay on the properties of water
Complete the worksheet on TLC of amino acids
Carry out separation of DNA using gel electrophoresis
Calculate the Rf values from your TLC worksheet and write a conclusion
Complete the worksheet showing the structure of Lipids and their function
3
Unit 13 Biochemistry and biochemical techniques
BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)
This brief has been verified as being fit for purpose
Assessor
Mrs Turner-Smith
Signature
Internal verifier
Signature
4
Date
06/07/2015
Date
06/07/2015
Ms Fensome
BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)
Unit 13 Biochemistry and biochemical techniques
Biological Molecules
Assessor's comments
Qualification
BTEC Level 3 Subsidiary
Diploma in Applied Science
Assessor
name
Unit number
and title
Unit 13: Biochemistry and
Biochemical Techniques
Learner name
Criteria
reference
To achieve the criteria the evidence must show that the learner
is able to:
Mrs N Turner-Smith
Achieved?
Identify the structure and function of biological molecules
P1
P2
Use laboratory separative techniques for the
characterisation of biological molecules
M1
Interpret data obtained from experiments designed to
separate biological molecules
D1
Discuss the relationship between structure and function
for triglycerides and phospholipids
Learner feedback
Assessor feedback
Assessor signature
Date
Learner signature
Date
5
Unit 13 Biochemistry and biochemical techniques
6
BTEC Level 3 Subsidiary Diploma in Applied Science
(Applied Biology)