IDENTIFICATION OF A BACTERIO
... 2. Radioactively labelled lysine, tyrosine and valine could not be ~corporated into peptide IV; the known sequence data show this peptide to lack these amino acids (as well as Ser, Phe, Asx). No other BrCN peptide lacks this combination of amino acids. 3. The amino acid analysis of purified peptide ...
... 2. Radioactively labelled lysine, tyrosine and valine could not be ~corporated into peptide IV; the known sequence data show this peptide to lack these amino acids (as well as Ser, Phe, Asx). No other BrCN peptide lacks this combination of amino acids. 3. The amino acid analysis of purified peptide ...
High Resolution Melt: species identification in theory and practice
... “In theory, theory and practice are the same. In practice, they are not.” This quotation from Albert Einstein describes well the theoretical expectations and the practical reality of High Resolution Melt (HRM) method, especially from the aspect of species identification in two-case-studies described ...
... “In theory, theory and practice are the same. In practice, they are not.” This quotation from Albert Einstein describes well the theoretical expectations and the practical reality of High Resolution Melt (HRM) method, especially from the aspect of species identification in two-case-studies described ...
II. Conversion Tables and Formulas
... RNA can be dried briefly at 37°C or in a vacuum oven. When working with RNA, place all samples on ice. For the reasons mentioned above, RNA is very susceptible to degradation when left at room temperature. Dissolve RNA by adding RNase-free buffer or water, then standing the tube on ice for 15 min. Ge ...
... RNA can be dried briefly at 37°C or in a vacuum oven. When working with RNA, place all samples on ice. For the reasons mentioned above, RNA is very susceptible to degradation when left at room temperature. Dissolve RNA by adding RNase-free buffer or water, then standing the tube on ice for 15 min. Ge ...
Supplementary Information (docx 4776K)
... quickly transferred to five flasks, and PP was sterilely added to each flask to achieve the final concentrations of 0, 100, 500, 2000, and 10000 μM respectively. Samples of ...
... quickly transferred to five flasks, and PP was sterilely added to each flask to achieve the final concentrations of 0, 100, 500, 2000, and 10000 μM respectively. Samples of ...
DNA Profiling - Mrs. Blackmon`s Science Blackboard
... specific person. • Small amounts may be only trace evidence • Polymerase chain reaction (PCR) technique generates multiple copies of DNA evidence ...
... specific person. • Small amounts may be only trace evidence • Polymerase chain reaction (PCR) technique generates multiple copies of DNA evidence ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.