One of the most famous examples
... As an introduction into an example of the function of proteins, let's consider first a special class of proteins that do not follow some of the rules we have have seen that govern protein structure in general. These are the MEMBRANE PROTEINS, the proteins that reside, not in the aqueous environment ...
... As an introduction into an example of the function of proteins, let's consider first a special class of proteins that do not follow some of the rules we have have seen that govern protein structure in general. These are the MEMBRANE PROTEINS, the proteins that reside, not in the aqueous environment ...
Tth RecA
... Tth RecA is a RecA homolog isolated from Thermus thermophilus. It has a ssDNA-dependent ATPase activity at an optimal temperature between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid ...
... Tth RecA is a RecA homolog isolated from Thermus thermophilus. It has a ssDNA-dependent ATPase activity at an optimal temperature between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated temperature condition, such as nucleic acid ...
Total RNA MinElute Cleanup - Yale Center for Genome Analysis
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
... 4. Transfer RNeasy MinElute column into a new 2 ml collection tube. Save flow through until sample quantitation is completed. 5. Pipet 500 μl of Buffer RPE onto column. Spin for 15 sec at ≥10,000 rpm. Discard flowthrough. 6. Pipette 500 μl of 80% ethanol to column. Centrifuge for 2 min at ≥10,000 rp ...
Untitled
... Generation Sequencing. Non toxic solution that allows the storage of saliva at room temperature, preserving and stabilizing DNA for its following extraction. ...
... Generation Sequencing. Non toxic solution that allows the storage of saliva at room temperature, preserving and stabilizing DNA for its following extraction. ...
Sodium dodecyl sulfate (L3771)
... The Electrophoresis grade SDS is tested for suitability as a reagent in electrophoresis. This SDS does not contain any contaminating ions that might impair proper electrophoretic mobility of test samples. SDS is an anionic detergent and wetting agent that is ...
... The Electrophoresis grade SDS is tested for suitability as a reagent in electrophoresis. This SDS does not contain any contaminating ions that might impair proper electrophoretic mobility of test samples. SDS is an anionic detergent and wetting agent that is ...
Protein Structure Analysis - G
... Sodium dodecyl sulfate (SDS) is commonly used for denaturing proteins into their constituent subunits or polypeptides and the method is known as sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE). In a SDS-polyacrylamide gel, the protein mixture is denatured by heating at 100 ...
... Sodium dodecyl sulfate (SDS) is commonly used for denaturing proteins into their constituent subunits or polypeptides and the method is known as sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (SDS-PAGE). In a SDS-polyacrylamide gel, the protein mixture is denatured by heating at 100 ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.