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Tth RecA
Developed and produced by BioHelix Corp., a NEB-affiliated company.
This product was discontinued on 01/27/2016
Catalog #
Size
Concentration
Categories: Discontinued
Product
Information
Protocols &
Manuals
Quality &
Safety
Legal
Information
Description
Notes
Properties and Usage
References
Description
Tth RecA is a RecA homolog isolated from Thermus thermophilus. It has a ssDNA-dependent ATPase activity at an optimal temperature
between 65–75°C. The extreme thermostability makes Tth RecA ideal for molecular biology applications that require an elevated
temperature condition, such as nucleic acid amplification and sequencing.
Highlights
Extreme thermostability
Nucleic acid amplification and sequencing
Isolated from a recombinant source
Product Source
An E. coli strain that carries the cloned RecA gene from Thermus thermophilus
Properties and Usage
Unit Definition
Sold by mass of pure protein as determined by OD280.
Storage Temperature
-20°C
Storage Conditions
10 mM Tris-HCl
100 mM KCl
1 mM DTT
0.1 mM EDTA
50% Glycerol
0.1% Triton® X-100
pH 7.5 @ 25°C
Heat Inactivation
No
Notes
1. Tth RecA is active in any polymerase buffer. Add 400 ng of the Tth RecA per 50 µl reaction.
References
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Wasserman, S.A. and Cozzarelli, N.R. (1985). Proc. Natl. Acad. Sci. USA. 82, 1079-1083.
Biet, E. et al. (2001). Biochemistry. 40, 1779-1786.
Shortle, D. et al. Proc. Natl. Acad. Sci. USA. 77, 5375-5379.
Rigas, B. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9591-9595.
Honigberg, S.M. et al. (1986). Proc. Natl. Acad. Sci. USA. 83, 9586-9590.
Koob, M. et al. (1992). Nucleic Acids Res.. 20, 5831-5836.
Shigemori, Y. et al. (2005). Nucleic Acids Res.. e126.
Datacards
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M2402Datasheet-Lot0061207
M2402Datasheet-Lot0061211
M2402Datasheet-Lot0061304
M2402Datasheet-Lot0071308
M2402Datasheet-Lot0081401
M2402Datasheet-Lot0081407
M2402Datasheet-Lot0711508
Quality Control
Datacards
Safety Data Sheet
Quality Control
Quality Assurance Statement
Each lot is tested for its ability to form a stable triple helix and is visually determined to be > 99% pure on an
SDSpolyacrylamide gel.
Quality Control Assays
The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product.
Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting
Documents section of this page. Further information regarding NEB product quality can be found here.
Endonuclease Activity (Nicking):
The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 4 hours the percent converted to
the nicked form is determined by agarose gel electrophoresis.
Exonuclease Activity (Radioactivity Release):
The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4
hours the exonuclease activity is determined by the % release of radioactive nucleotides.
Non-Specific DNase Activity (16 hour):
The product is tested for non-specific nuclease degradation in a reaction containing a DNA substrate. After incubation for 16
hours there is no detectable degradation of the DNA substrate as determined by agarose gel electrophoresis.
Safety Data Sheet
The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.
Tth RecA
Datacards
The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and
quality controls. The following file naming structure is used to name the majority of these document files: [Catalog
Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the
Technical Support Form for appropriate document.
M2402Datasheet-Lot0061207
M2402Datasheet-Lot0061211
M2402Datasheet-Lot0061304
M2402Datasheet-Lot0071308
M2402Datasheet-Lot0081401
M2402Datasheet-Lot0081407
M2402Datasheet-Lot0711508
Legal and Disclaimers
Legal and Disclaimers
This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc
(NEB).
While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain
additional third party intellectual property rights for certain applications.
For more information about commercial rights, please contact NEB's Global Business Development team at [email protected].
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes
in humans or animals.