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Home › Products › Adenosine 5'-Triphosphate (ATP) Adenosine 5'-Triphosphate (ATP) Did you know this product can be customized or purchased in larger volumes? Submit an inquiry to find out more about customization options. Catalog # P0756S Size 1 ml Concentration 10 mM Price $33.00 Qty P0756L 5 ml 10 mM $132.00 1 Categories: Nucleotide Solution Products, Nucleotide Solution Products Applications: DNA Ligation, In vitro Synthesis & Processing, Protein Phosphatases & Kinases | More + Product Information Protocols & Manuals Other Tools & Resources Quality & Safety Description Notes Legal Information Properties and Usage References Description Adenosine 5´-Triphosphate (ATP) is a substrate for many ATP-dependent enzyme systems (1). Product Source Isolated from a microbial source. Properties and Usage Storage Temperature -20°C Molecular Weight Theoretical: 551.2 daltons Storage Notes For long term storage (>30 days), store at -80°C. Notes 1. Storage: Sterile purified water adjusted to pH 7.0 with NaOH. 2. For long term storage, keep in small aliquots at -70°C. References 1. Knowles, J.R. (1980). 49, 479-919. Annie Rev. Biochem.. 1 Datacards Datacards The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document. P0756Datasheet-Lot0081204 P0756Datasheet-Lot0081207 P0756Datasheet-Lot0081212 P0756Datasheet-Lot0091306 P0756Datasheet-Lot0101401 P0756Datasheet-Lot0111406 P0756Datasheet-Lot0111409 P0756Datasheet-Lot0121503 P0756Datasheet-Lot0121510 P0756Datasheet-Lot0131603 P0756Datasheet-Lot0131610 Usage Guidelines & Tips Interactive Tools Troubleshooting Guides Usage Guidelines & Tips Tips for Maximizing Ligation Efficiencies Troubleshooting Guides Troubleshooting Tips for Ligation Reactions Interactive Tools NEBioCalculator Quality Control Datacards Safety Data Sheet Quality Control Quality Control Assays The following Quality Control Tests are performed on each new lot and meet the specifications designated for the product. Individual lot data can be found on the Product Summary Sheet/Datacard or Manual which can be found in the Supporting Documents section of this page. Further information regarding NEB product quality can be found here. Endonuclease Activity (Nicking): The product is tested in a reaction containing a supercoiled DNA substrate. After incubation for 16 hours the percent converted to the nicked form is determined by agarose gel electrophoresis. Exonuclease Activity (Radioactivity Release): The product is tested in a reaction containing a radiolabeled mixture of single and double-stranded DNA. After incubation for 4 hours the exonuclease activity is determined by the % release of radioactive nucleotides. Non-Specific RNase Activity (16 hour, RNA): The RNA is tested in a reaction under standard reaction conditions. After incubation for 16 hours there is no detectable degradation of the RNA as determined by agarose gel electrophoresis. Phosphatase Activity (Oligo): The product is tested in a reaction containing a 5'-phosphorylated oligo. After incubation there is no detectable dephosphorylation as determined by SDS-PAGE Protease Activity (SDS-PAGE): The product is tested for protease activity by incubation with a standard mixture of proteins resulting in no detectable degradation of the proteins as determined by SDS-PAGE. Safety Data Sheet The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely. Adenosine 5'-Triphosphate (ATP) Datacards The Product Summary Sheet, or Data Card, includes details for how to use the product, as well as details of its formulation and quality controls. The following file naming structure is used to name the majority of these document files: [Catalog Number]Datasheet-Lot[Lot Number]. For those product lots not listed below, please contact NEB at [email protected] or fill out the Technical Support Form for appropriate document. P0756Datasheet-Lot0081204 P0756Datasheet-Lot0081207 P0756Datasheet-Lot0081212 P0756Datasheet-Lot0091306 P0756Datasheet-Lot0101401 P0756Datasheet-Lot0111406 P0756Datasheet-Lot0111409 P0756Datasheet-Lot0121503 P0756Datasheet-Lot0121510 P0756Datasheet-Lot0131603 P0756Datasheet-Lot0131610 Legal and Disclaimers Legal and Disclaimers This product is covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). While NEB develops and validates its products for various applications, the use of this product may require the buyer to obtain additional third party intellectual property rights for certain applications. For more information about commercial rights, please contact NEB's Global Business Development team at [email protected]. This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
