Nucleic Acid structure - part 1
... Discovery of DNA structure and its role in housing genetic information Avery-MacLeod-McCarty 1944 Direct evidence that DNA carries genetic info ...
... Discovery of DNA structure and its role in housing genetic information Avery-MacLeod-McCarty 1944 Direct evidence that DNA carries genetic info ...
NZY First-Strand cDNA Synthesis Kit
... RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H min ...
... RNA. The kit includes a combination of random hexamers and oligo(dT)18 primers in order to increase sensitivity. The primers are included in the NZYRT 2× Master Mix, which also contains dNTPs, MgCl2 and an optimized RT buffer. NZYRT Enzyme Mix includes both the NZY Reverse Transcriptase (RNase H min ...
President`s DNA Initiative – Analyst Training
... • Run temperature -- 60 oC helps reduce secondary structure on DNA and improves precision • Electrophoresis buffer -- urea in running buffer helps keep DNA strands denatured • Capillary wall coating -- dynamic coating with polymer ...
... • Run temperature -- 60 oC helps reduce secondary structure on DNA and improves precision • Electrophoresis buffer -- urea in running buffer helps keep DNA strands denatured • Capillary wall coating -- dynamic coating with polymer ...
Aromatic compounds of biological importance
... subunits with respect to one another. Interaction between subunits is mediated by noncovalent forces, such as hydrogen bonds and hydrophobic interactions. ...
... subunits with respect to one another. Interaction between subunits is mediated by noncovalent forces, such as hydrogen bonds and hydrophobic interactions. ...
chemical modification of carboxylic groups
... SDS. It seemed thus very likely that the numerous negative charges of the protein hindered an optimal fixation of SDS and that Actinomadura R39 /3-lactamase bound much less SDS per gram than do most polypeptides. On that basis, the remarkably low electrophoretic mobility of the Actinomnadura R39 /3- ...
... SDS. It seemed thus very likely that the numerous negative charges of the protein hindered an optimal fixation of SDS and that Actinomadura R39 /3-lactamase bound much less SDS per gram than do most polypeptides. On that basis, the remarkably low electrophoretic mobility of the Actinomnadura R39 /3- ...
Structure of a protein - Campus
... the twisting of the protein chains due to the formation of bonds between amino acid residual groups that are distant from each other and in association with the presence of nontwisted sections that form the pivot for any folding. ...
... the twisting of the protein chains due to the formation of bonds between amino acid residual groups that are distant from each other and in association with the presence of nontwisted sections that form the pivot for any folding. ...
Proteinase K, solution
... Proteinase K (CAS: 39450-01-6) is a non-specific serine protease having a very high specific activity (cleaves the carboxylic ends of aromatic, hydrophobic and aliphatic amino acids). It has been used for isolation of mRNA, high molecular weight DNA and to inactivate other enzymatic activities. Prot ...
... Proteinase K (CAS: 39450-01-6) is a non-specific serine protease having a very high specific activity (cleaves the carboxylic ends of aromatic, hydrophobic and aliphatic amino acids). It has been used for isolation of mRNA, high molecular weight DNA and to inactivate other enzymatic activities. Prot ...
What are proteins - Assiut University
... Color change proportional to [protein] Bradford, Lowry, BCA ...
... Color change proportional to [protein] Bradford, Lowry, BCA ...
Datasheet for T4 RNA Ligase 1 (ssRNA Ligase), High Concentration
... Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´[32P rA16 into a phosphatase-resistant form in 30 minutes at 37°C Unit Assay Conditions: 1X T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of 5´-[32P]rA1 ...
... Unit Definition: One unit is defined as the amount of enzyme required to convert 1 nanomole of 5´[32P rA16 into a phosphatase-resistant form in 30 minutes at 37°C Unit Assay Conditions: 1X T4 RNA Ligase reaction buffer, supplemented with 1 mM ATP, is mixed with the RNA substrate (10µM of 5´-[32P]rA1 ...
ATAC-Seq - NeuroLINCS
... transposase is used to interrogate chromatin accessibility by inserting high-throughput DNA sequencing adapters into open genomic regions, which allows for the preferential amplification of DNA fragments located at sites of active chromatin. The ATAC-Seq protocol was adapted from Buenrostro et al. ( ...
... transposase is used to interrogate chromatin accessibility by inserting high-throughput DNA sequencing adapters into open genomic regions, which allows for the preferential amplification of DNA fragments located at sites of active chromatin. The ATAC-Seq protocol was adapted from Buenrostro et al. ( ...
Functional proteome analysis of wheat: systematic classification of
... 2009a,b). Protein spots in 2-DE gels were visualized by Coomassie Brilliant Blue (CBB) R-250 staining (Woo et al. 2002). Each sample was run three times and the best visualized gels were selected. In-gel digestion and mass spectrometry analysis Selected protein spots were excised from preparative lo ...
... 2009a,b). Protein spots in 2-DE gels were visualized by Coomassie Brilliant Blue (CBB) R-250 staining (Woo et al. 2002). Each sample was run three times and the best visualized gels were selected. In-gel digestion and mass spectrometry analysis Selected protein spots were excised from preparative lo ...
Supporting Information S1: 1. Establishment of hSMP30 transcription
... cooled to room temp and was reverse transcribed at 42ºC for 1hour using primer extension system (Promega, USA) according to manufacturer’s instruction. The same primer was used for the sequencing reactions of cloned SMP30 promoter containing exon 1. Sequencing reactions and primer extension product ...
... cooled to room temp and was reverse transcribed at 42ºC for 1hour using primer extension system (Promega, USA) according to manufacturer’s instruction. The same primer was used for the sequencing reactions of cloned SMP30 promoter containing exon 1. Sequencing reactions and primer extension product ...
PDF
... determination. PCR amplicons containing the potentially edited genomic locus are used in a cleavage reaction set up with the same sgRNA as was used for the initial gene editing. The cleavage products are then separated on an agarose gel. If both alleles of the target site are wild type (wt), the Cas ...
... determination. PCR amplicons containing the potentially edited genomic locus are used in a cleavage reaction set up with the same sgRNA as was used for the initial gene editing. The cleavage products are then separated on an agarose gel. If both alleles of the target site are wild type (wt), the Cas ...
PDF Links - Journal of the Korean Ceramic Society
... the HAp phase.7) The 1339 cm-1 band of GEL is red-shifted in HAp/GEL nanocomposites. The lower chemical shift of 1339 cm-1 band is caused by the smaller crystallites of HAp bound with GEL macromolecules, suggesting the lower energy wagging of the proline side chain. The proline band peak of HG2Asn a ...
... the HAp phase.7) The 1339 cm-1 band of GEL is red-shifted in HAp/GEL nanocomposites. The lower chemical shift of 1339 cm-1 band is caused by the smaller crystallites of HAp bound with GEL macromolecules, suggesting the lower energy wagging of the proline side chain. The proline band peak of HG2Asn a ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.