basic laboratory course 3
... A major goal in the modern biology laboratory is the isolation of specific proteins from complex mixtures. Such isolation procedures frequently involve chromatography. Proteins are most often separated by column chromatography, in which a mixture of different proteins is passed through a column or t ...
... A major goal in the modern biology laboratory is the isolation of specific proteins from complex mixtures. Such isolation procedures frequently involve chromatography. Proteins are most often separated by column chromatography, in which a mixture of different proteins is passed through a column or t ...
Macromolecules: Their Structure and Function A. Lipids: Water
... • The tertiary structure is generated by bending and folding of the polypeptide chain. • The quaternary structure is the arrangement of polypeptides in a single functional unit consisting of more than one polypeptide subunit. Figure 3.5 – Part 2 ...
... • The tertiary structure is generated by bending and folding of the polypeptide chain. • The quaternary structure is the arrangement of polypeptides in a single functional unit consisting of more than one polypeptide subunit. Figure 3.5 – Part 2 ...
RNA - GVI.cz
... synthesis (= synthesis of proteins) synthesis of proteins = protein synthesis : fundamental process, by which the information from DNA is transferred into a particular sign polynucleotide chain ...
... synthesis (= synthesis of proteins) synthesis of proteins = protein synthesis : fundamental process, by which the information from DNA is transferred into a particular sign polynucleotide chain ...
Amino Acids
... Electrophoresis for Protein Analysis Separation in analytical scale is commonly done by electrophoresis – Electric field pulls proteins according to their charge – Gel matrix hinders mobility of proteins according to their size and shape ...
... Electrophoresis for Protein Analysis Separation in analytical scale is commonly done by electrophoresis – Electric field pulls proteins according to their charge – Gel matrix hinders mobility of proteins according to their size and shape ...
PCRBIO Taq DNA Polymerase
... PCRBIO Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA. PCRBIO Taq DNA Polymerase is a robust ...
... PCRBIO Taq DNA Polymerase uses the latest developments in polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system allow for superior PCR performance on complex templates such as mammalian genomic DNA. PCRBIO Taq DNA Polymerase is a robust ...
Unit 1.1 Building Blocks of Life The student knows the significance of
... occur in living organisms. The student is expected to: ...
... occur in living organisms. The student is expected to: ...
Southern Blotting and Related DNA Detection Techniques
... The system shown in Figure 1 is an accurate description of Southern blotting as still carried out in many laboratories, but various modifications have been introduced over the years to improve the efficiency of DNA transfer from gel to membrane. The major improvement has been the introduction of nylon ...
... The system shown in Figure 1 is an accurate description of Southern blotting as still carried out in many laboratories, but various modifications have been introduced over the years to improve the efficiency of DNA transfer from gel to membrane. The major improvement has been the introduction of nylon ...
Biology 3.2
... – Two monosaccharides join to form a double sugar called a disaccharide. – A complex sugar, or polysaccharide, is made of ...
... – Two monosaccharides join to form a double sugar called a disaccharide. – A complex sugar, or polysaccharide, is made of ...
Key Molecule for the Evolution of Life—Nucleic Acid
... The principle of structure formation lies in the baseof both information and function? Rationally speaking, the pairing for both of double helix and hairpin loop structures. secret must be within the molecule structure. In fact we can The former can be said to be a complete type of the basefind the ...
... The principle of structure formation lies in the baseof both information and function? Rationally speaking, the pairing for both of double helix and hairpin loop structures. secret must be within the molecule structure. In fact we can The former can be said to be a complete type of the basefind the ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.