Forever 100bp Ladder Personalizer
... ♦ Lifetime usage: sufficient template and primers are supplied to provide enough markers for about a 20 year supply in the typical laboratory. If needed, the plasmids can be transformed to yield an unlimited supply of templates and the primer sequences are supplied so that you can replace them if ne ...
... ♦ Lifetime usage: sufficient template and primers are supplied to provide enough markers for about a 20 year supply in the typical laboratory. If needed, the plasmids can be transformed to yield an unlimited supply of templates and the primer sequences are supplied so that you can replace them if ne ...
Tagging the Rolling Circle Products with Nanocrystal Clusters for
... precipitated with centrifugation at 16.1k X g for 12 min and dispersed in 100 μL of linking buffer. ZnSstreptavidin products were blocked by 10 mL of 5% BSA/PBS solution before being used in the assay to reduce the nonspecific adsorption. ...
... precipitated with centrifugation at 16.1k X g for 12 min and dispersed in 100 μL of linking buffer. ZnSstreptavidin products were blocked by 10 mL of 5% BSA/PBS solution before being used in the assay to reduce the nonspecific adsorption. ...
Biological Molecules: Structure and Methods of Analysis
... visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, and these are compared to DNA standards (known sizes) which are also present in the gel. This allows you to identify the size of the DNA or RNA in your samples, but it does not reveal anything about the sequen ...
... visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, and these are compared to DNA standards (known sizes) which are also present in the gel. This allows you to identify the size of the DNA or RNA in your samples, but it does not reveal anything about the sequen ...
Biological Molecules: Structure and Methods of Analysis
... visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, and these are compared to DNA standards (known sizes) which are also present in the gel. This allows you to identify the size of the DNA or RNA in your samples, but it does not reveal anything about the sequen ...
... visualize the DNA or RNA. The locations of the DNA or RNA appear as bands within the gel, and these are compared to DNA standards (known sizes) which are also present in the gel. This allows you to identify the size of the DNA or RNA in your samples, but it does not reveal anything about the sequen ...
Polymerase Chain Reaction (PCR)
... through a firm gel which is really a sieve with small holes of a fixed size – Phosphate group in DNA is negatively charged so it is moved towards a positive electrode by the current – Longer fragments have more nucleotides • So have a larger molecular weight • So are bigger in size • So aren’t able ...
... through a firm gel which is really a sieve with small holes of a fixed size – Phosphate group in DNA is negatively charged so it is moved towards a positive electrode by the current – Longer fragments have more nucleotides • So have a larger molecular weight • So are bigger in size • So aren’t able ...
(mmg) operon of Bacillus
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
... various industries. Hence this organism is extensively studied. The genome of this bacterium has around 4000 protein coding sequences, which include 87% of the genome sequence. Because of its ability to use different carbohydrates, the glycolytic pathway along with the TCA cycle is utilized in this ...
363 Isoelectric focusing of indoleacetic acid degrading enzymes
... G-100. The first 8 fractions after the passage of the void volume ...
... G-100. The first 8 fractions after the passage of the void volume ...
Proteolytic Enzymes in Detergents: Evidence of Their
... Although this could be attributed to denaturation of the enzyme in this acidic pH, it is more likely due to the protonation state of the catalytic triad (the protonated histidine cannot extract the proton from serine). Control reactions without the enzyme can also be carried out if desired. In this ...
... Although this could be attributed to denaturation of the enzyme in this acidic pH, it is more likely due to the protonation state of the catalytic triad (the protonated histidine cannot extract the proton from serine). Control reactions without the enzyme can also be carried out if desired. In this ...
Genomic DNA Extraction From Buccal Epithelial Cells
... Day ONE: Collecting your DNA sample and running PCR 1. Obtain a Chelex tube. Note that this tube is identified with a number. Record this number in your notebook. Only you will know this anonymous code. 2. To collect buccal epithelial cells, use a sterile toothpick or sterile pipette tip to gently s ...
... Day ONE: Collecting your DNA sample and running PCR 1. Obtain a Chelex tube. Note that this tube is identified with a number. Record this number in your notebook. Only you will know this anonymous code. 2. To collect buccal epithelial cells, use a sterile toothpick or sterile pipette tip to gently s ...
RNA Secondary Structure Based Prediction of Simian
... regions, the secondary structures were predicted to find several stem-loops. Gel shift assay showed that some SIV candidate stem-loops had relatively high binding affinity with NCp8. Although further experiments are required, they are considered to be one of the NCp8 recognition site. In the case of DN ...
... regions, the secondary structures were predicted to find several stem-loops. Gel shift assay showed that some SIV candidate stem-loops had relatively high binding affinity with NCp8. Although further experiments are required, they are considered to be one of the NCp8 recognition site. In the case of DN ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.