PR Reagent (Plant Total RNA Isolation Kit)
... The PR Reagent provides an easy 3-step method to isolate the total RNA from plant samples. This unique reagent system ensures the total RNA with a high yield and good quality from the most common plant samples as well as samples high in polysaccharides. If a larger sample is required, the kit volume ...
... The PR Reagent provides an easy 3-step method to isolate the total RNA from plant samples. This unique reagent system ensures the total RNA with a high yield and good quality from the most common plant samples as well as samples high in polysaccharides. If a larger sample is required, the kit volume ...
The Electrophoretic Movement of Proteins from Various
... pattern even after 8 days, provided that bacterial contamination was avoided. I n practice, all the runs on any one extract were completed within 3 days. The ability to store these extracts allows the comparison on the same day of cultures harvested at different times. Other investigators (Chang et ...
... pattern even after 8 days, provided that bacterial contamination was avoided. I n practice, all the runs on any one extract were completed within 3 days. The ability to store these extracts allows the comparison on the same day of cultures harvested at different times. Other investigators (Chang et ...
Designing and making sgRNA constructs
... cloning backbone, the example guide sequence one base ‘G’ followed by 19 Ns, instead of the 20 Ns shown for the PX260/334 cloning backbones. This difference in oligo design was due to the requirement of human U6 promoter to have a ‘G’ base at the transcription start site. Hence, we recommend finding ...
... cloning backbone, the example guide sequence one base ‘G’ followed by 19 Ns, instead of the 20 Ns shown for the PX260/334 cloning backbones. This difference in oligo design was due to the requirement of human U6 promoter to have a ‘G’ base at the transcription start site. Hence, we recommend finding ...
Supplementary information (SI) Description of technique The
... amplified in eight separate 25uL LR-PCR reactions (Roche, Long Range Expand kit) using PCR primers (Table S2). Two overlapping 8Kb fragments were generated spanning 16,569bp. In total 42 cycles of PCR were carried out using the Bio-Rad Tetrad Thermal Cycler. Cycling conditions included: initial dena ...
... amplified in eight separate 25uL LR-PCR reactions (Roche, Long Range Expand kit) using PCR primers (Table S2). Two overlapping 8Kb fragments were generated spanning 16,569bp. In total 42 cycles of PCR were carried out using the Bio-Rad Tetrad Thermal Cycler. Cycling conditions included: initial dena ...
Plant RNA/DNA Purification Kit
... however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more re ...
... however this novel technology will allow for their simultaneous isolation from the same sample. This will not only save time, but will also be of a great benefit when isolating RNA and DNA from precious, difficult to obtain or very small samples. Furthermore, gene expression analysis will be more re ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.