A simple and improved PCR-based technique for white
... cross-species application of similar zinc-finger based techniques (Morin et al. 2005; Shaw et al. 2003; Wilson and White 1998). Although previous studies have provided more complicated protocols to distinguish sexes of whitetailed deer tissue samples, this technique provides several advantages for r ...
... cross-species application of similar zinc-finger based techniques (Morin et al. 2005; Shaw et al. 2003; Wilson and White 1998). Although previous studies have provided more complicated protocols to distinguish sexes of whitetailed deer tissue samples, this technique provides several advantages for r ...
Restriction mapping
... modification system. Today, most commercially available REs are not purified from their natural sources.. Instead, REs are usually isolated from bacteria that overexpress large quantities of REs from plasmids. These recombinant REs have often been engineered by molecular biologists to include amino ...
... modification system. Today, most commercially available REs are not purified from their natural sources.. Instead, REs are usually isolated from bacteria that overexpress large quantities of REs from plasmids. These recombinant REs have often been engineered by molecular biologists to include amino ...
Detection of Viral, Bacterial and Human Genomic DNA
... For visual analysis, 10 L of DNA from the final DNA elution was loaded onto a 1% agarose TAE gel and run for 25 minutes at 150 V alongside Norgen’s HighRanger 1 kb DNA ladder (Cat. 11900). The gel photo was taken using an AlphaImagerTM IS-2200 (Alpha Innotech). PCR Amplification The purified DNA wa ...
... For visual analysis, 10 L of DNA from the final DNA elution was loaded onto a 1% agarose TAE gel and run for 25 minutes at 150 V alongside Norgen’s HighRanger 1 kb DNA ladder (Cat. 11900). The gel photo was taken using an AlphaImagerTM IS-2200 (Alpha Innotech). PCR Amplification The purified DNA wa ...
Ion exchange chromatography
... Bauman as a new analytical method. In 1979, Fritz et al. described an alternative separation and detection scheme for inorganic anions, in which the separator column is directly coupled to the conductivity cell. At the end of the 1970s, ion chromatographic techniques were used to analyze organic ion ...
... Bauman as a new analytical method. In 1979, Fritz et al. described an alternative separation and detection scheme for inorganic anions, in which the separator column is directly coupled to the conductivity cell. At the end of the 1970s, ion chromatographic techniques were used to analyze organic ion ...
Developing a Novel Means of Observing the
... post-transcriptional control of a specific range of mRNAs (Liu & Szaro, 2011). During neural development, the protein helps to coordinate the synthesis of structural proteins used to organize microtubules, microfilaments, and NFs [neurofilaments] into an axon through its export out of the nucleus. H ...
... post-transcriptional control of a specific range of mRNAs (Liu & Szaro, 2011). During neural development, the protein helps to coordinate the synthesis of structural proteins used to organize microtubules, microfilaments, and NFs [neurofilaments] into an axon through its export out of the nucleus. H ...
Extraction of High Molecular Weight Genomic DNA from Soils and
... environmental genomic libraries for downstream sequencing and screening applications. The procedure starts with cell lysis. Cell walls and membranes of microbes are lysed by both mechanical (grinding) and chemical forces (β-mercaptoethanol). Genomic DNA is then isolated using extraction buffer, chlo ...
... environmental genomic libraries for downstream sequencing and screening applications. The procedure starts with cell lysis. Cell walls and membranes of microbes are lysed by both mechanical (grinding) and chemical forces (β-mercaptoethanol). Genomic DNA is then isolated using extraction buffer, chlo ...
INFORMATION FOR FOREIGN STUDENTS
... between the supporting medium and the insulating plate. 2. Pencil a "start" mark on a dry cellulose acetate membrane and immerse it slowly into a cuvette filled with a buffer in such a way that it will absorb the fluid only from below, by capillary forces, for its rapid immersion traps air bubbles i ...
... between the supporting medium and the insulating plate. 2. Pencil a "start" mark on a dry cellulose acetate membrane and immerse it slowly into a cuvette filled with a buffer in such a way that it will absorb the fluid only from below, by capillary forces, for its rapid immersion traps air bubbles i ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.