Detection and characterization of gamete‐specific molecules in
... HEIKO STUCKAS,1* KATRIN MESSERSCHMIDT,2 SASCHA PUTZLER,2 OTTO BAUMANN,3 JÖRG A. SCHENK,4 ...
... HEIKO STUCKAS,1* KATRIN MESSERSCHMIDT,2 SASCHA PUTZLER,2 OTTO BAUMANN,3 JÖRG A. SCHENK,4 ...
A single-molecule FRET sensor for monitoring DNA synthesis in real
... 1) Hand selected time traces that indicated one or more polymerization events were loaded into ebFRET. 2) The selected time traces are filtered for photo bleaching: Parts that indicated a bleached acceptor were excluded from further analysis. 3) Due to the long duration of measurements, some traces ...
... 1) Hand selected time traces that indicated one or more polymerization events were loaded into ebFRET. 2) The selected time traces are filtered for photo bleaching: Parts that indicated a bleached acceptor were excluded from further analysis. 3) Due to the long duration of measurements, some traces ...
Programmed Cell Death during Leaf Senescence in Eucommia
... 15 min at 37℃ and rinsed twice with PBS. Then sections were stained for PCD observation with in situ cell death detection kit, AP (Boehringer, Mannheim, Germany). Reaction products were dark blue. In positive control, DNase I (grade I, 0.5 mg/ml in 50 mmol/L Tris-HCl, pH 7.5, 1 mg/ml BSA) was added ...
... 15 min at 37℃ and rinsed twice with PBS. Then sections were stained for PCD observation with in situ cell death detection kit, AP (Boehringer, Mannheim, Germany). Reaction products were dark blue. In positive control, DNase I (grade I, 0.5 mg/ml in 50 mmol/L Tris-HCl, pH 7.5, 1 mg/ml BSA) was added ...
Rapid sequencing of DNA based on single molecule detection
... photostability. We have been able to utilize the increased photon yield of R6G in EtOH to observe the bursts of photons from individual molecules of R6G using cW excitation as indicated from non-random correlations in the autocorrelation function and tails in the Poisson distributions (13). For TRIT ...
... photostability. We have been able to utilize the increased photon yield of R6G in EtOH to observe the bursts of photons from individual molecules of R6G using cW excitation as indicated from non-random correlations in the autocorrelation function and tails in the Poisson distributions (13). For TRIT ...
Determination of Molecular Weight of
... has played a critical role in laying the foundations for modern molecular biology. Among its achievements is the demonstration that proteins are macromolecules rather than complexes of smaller units, and direct support for the semiconservative replication mechanism of DNA as proposed by Watson and C ...
... has played a critical role in laying the foundations for modern molecular biology. Among its achievements is the demonstration that proteins are macromolecules rather than complexes of smaller units, and direct support for the semiconservative replication mechanism of DNA as proposed by Watson and C ...
Serological and molecular techniques to detect and identify plant
... agents by biological tests. The sensitivity of biological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. It may take f 0-450 particles of a virus to successfully initiate a new infection. Inoculation with fewer particles could result in a fal ...
... agents by biological tests. The sensitivity of biological tests is usually determined by the minimum number of pathogen propagules required to initiate infection. It may take f 0-450 particles of a virus to successfully initiate a new infection. Inoculation with fewer particles could result in a fal ...
Lab IV: Recombinant DNA Analysis
... separating the fragments by size on an agarose gel, and determining their sizes. For more information about the sequences recognized by the restriction enzymes that we will use, see the websites of biotechnology firms such as New England Biolabs (www.neb.com). ...
... separating the fragments by size on an agarose gel, and determining their sizes. For more information about the sequences recognized by the restriction enzymes that we will use, see the websites of biotechnology firms such as New England Biolabs (www.neb.com). ...
Methods and approaches for the comprehensive characterization
... then compared with publicly available protein sequence information. The observed masses of the proteolytic fragments are compared with theoretical in silico sequences to identify the peptide sequence and thereby the protein (14, 29, 36, 44, 65, 77). With the advancements in the separation techniques ...
... then compared with publicly available protein sequence information. The observed masses of the proteolytic fragments are compared with theoretical in silico sequences to identify the peptide sequence and thereby the protein (14, 29, 36, 44, 65, 77). With the advancements in the separation techniques ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.