Summer Internship project
... The use of RNA measurements to estimate the abundance of microorganisms in samples would be both powerful and convenient. Combined with gene expression analysis, a single RNA extraction would provide answers to a number of different questions: (i) How many microorganisms are present?; (ii) What type ...
... The use of RNA measurements to estimate the abundance of microorganisms in samples would be both powerful and convenient. Combined with gene expression analysis, a single RNA extraction would provide answers to a number of different questions: (i) How many microorganisms are present?; (ii) What type ...
Using Genes for Antibiotic Resistance to Trace Sources of Bacterial
... geographical locations. It is important to know if the same gene caused this contamination, so that the industry can take the first steps towards solving the problem.2 Our hypothesis was that there was some shared source of contamination. Even though the farms are sufficiently far apart, many farmer ...
... geographical locations. It is important to know if the same gene caused this contamination, so that the industry can take the first steps towards solving the problem.2 Our hypothesis was that there was some shared source of contamination. Even though the farms are sufficiently far apart, many farmer ...
Amino acid sequence of PR-39
... a fairly stoichiometric cleavage of the peptide was trypsin, but only at a high enzyme/substrate ratio (2 - 3 :5 ) . The tryptic fragments were separated on reverse-phase HPLC and the material corresponding to four peaks (T2, T3, T5, and T6; Fig. 4) were analyzed for amino acid composition and seque ...
... a fairly stoichiometric cleavage of the peptide was trypsin, but only at a high enzyme/substrate ratio (2 - 3 :5 ) . The tryptic fragments were separated on reverse-phase HPLC and the material corresponding to four peaks (T2, T3, T5, and T6; Fig. 4) were analyzed for amino acid composition and seque ...
Purification and some characteristics of a calcium
... Gel electrophoresis. Both denaturing (SDS) and non-denaturing PAGE were carried out on a 15% (w/v) resolving gel with the discontinuous system described by Laemmli (1970). The gels were stained with Coomassie brilliant blue R-250 (Sigma) or silver stain (Bio-Rad) depending on the protein concentrati ...
... Gel electrophoresis. Both denaturing (SDS) and non-denaturing PAGE were carried out on a 15% (w/v) resolving gel with the discontinuous system described by Laemmli (1970). The gels were stained with Coomassie brilliant blue R-250 (Sigma) or silver stain (Bio-Rad) depending on the protein concentrati ...
Molecular and Immunological Methods
... Adds a reporter molecule to a PCR reaction, allowing detection of the amplicon through the course of the PCR. This is the most important difference to conventional PCR methodologies. These reporter molecules are attached to primers, oligonucleotide probes, or the amplicon, conferring fluorescent pot ...
... Adds a reporter molecule to a PCR reaction, allowing detection of the amplicon through the course of the PCR. This is the most important difference to conventional PCR methodologies. These reporter molecules are attached to primers, oligonucleotide probes, or the amplicon, conferring fluorescent pot ...
Proteomics of spermatogenesis: from protein lists to understanding
... phosphostain (Invitrogen, Carlsbad, CA, USA) as a unique fluorescence-based detection system for the specific and sensitive analysis of protein and peptide phosphorylation status.24 A total of 68 spots representing 52 proteins were stained, thereby confirming the presence of phosphorylated forms of ...
... phosphostain (Invitrogen, Carlsbad, CA, USA) as a unique fluorescence-based detection system for the specific and sensitive analysis of protein and peptide phosphorylation status.24 A total of 68 spots representing 52 proteins were stained, thereby confirming the presence of phosphorylated forms of ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.