Your EasyGuide to DNA Polymerases
... suitable for effective integration into TA cloning vectors. BIOTAQ Red is an ideal choice for highthroughput applications, since it contains an inert red dye to facilitate easy recognition. When the reaction is complete, a sample of the Reaction Mix can be loaded directly onto agarose gels without t ...
... suitable for effective integration into TA cloning vectors. BIOTAQ Red is an ideal choice for highthroughput applications, since it contains an inert red dye to facilitate easy recognition. When the reaction is complete, a sample of the Reaction Mix can be loaded directly onto agarose gels without t ...
STRUCTURE AND FUNCTION
... column. A typical gel is composed of small polymeric beads with pores of varying size. As the solution flows through the gel column smaller proteins can enter the pores of the beads and are retarded while the larger proteins do not enter the pores and thus are eluted faster. ...
... column. A typical gel is composed of small polymeric beads with pores of varying size. As the solution flows through the gel column smaller proteins can enter the pores of the beads and are retarded while the larger proteins do not enter the pores and thus are eluted faster. ...
Chromatography
... Removal of salts and small molecules from macromolecules can be easily performed by using gel filtration . It is used in the determination of molecular weight of macromolecules . It is used for the purpose of separation of biological molecules leading to their ultimate purification . ...
... Removal of salts and small molecules from macromolecules can be easily performed by using gel filtration . It is used in the determination of molecular weight of macromolecules . It is used for the purpose of separation of biological molecules leading to their ultimate purification . ...
DNA Sequencing
... • The resulting terminated chains are resolved by electrophoresis. • Fragments from each of the four tubes are placed in four separate gel lanes. ...
... • The resulting terminated chains are resolved by electrophoresis. • Fragments from each of the four tubes are placed in four separate gel lanes. ...
Mechanism, and Role in Recombination Type-1
... is observed with single-stranded DNA and nicked DNA, we conclude that the enzyme recognizes a similar conformation in each case. The strong binding affinity of topoisomerase I for negatively supercoiled DNA is likely also due to its preference for an unwound region. We have suggested a model for rel ...
... is observed with single-stranded DNA and nicked DNA, we conclude that the enzyme recognizes a similar conformation in each case. The strong binding affinity of topoisomerase I for negatively supercoiled DNA is likely also due to its preference for an unwound region. We have suggested a model for rel ...
Proteomic profiling of non-obese type 2 diabetic skeletal muscle
... carefully placed on top of 12.5% (w/v) slab gels and electrophoresed in an Amersham Ettan DALT-Twelve system at 1.5 W per gel until the bromophenol blue dye front had just ran off the gel. The protein separation pattern on twodimensional gels was visualized by colloidal Coomassie Blue (29), silver ( ...
... carefully placed on top of 12.5% (w/v) slab gels and electrophoresed in an Amersham Ettan DALT-Twelve system at 1.5 W per gel until the bromophenol blue dye front had just ran off the gel. The protein separation pattern on twodimensional gels was visualized by colloidal Coomassie Blue (29), silver ( ...
RECOMBINANT-DNA METHODOLOGY
... all depends on the sequence of the specific piece of DNA in question. Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces of DNA. For example, a piece of DNA that is cut from a bigger piece of DNA i ...
... all depends on the sequence of the specific piece of DNA in question. Cutting with restriction endonucleases is very useful for moving specific pieces of DNA around from place to place. It’s also a useful way to name pieces of DNA. For example, a piece of DNA that is cut from a bigger piece of DNA i ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.