IMMUNOCHEMICAL TECHNIQUES Antigens Antibodies
... Polyspecific antibodies are sometimes incorrectly denoted as “polyvalent” and monospecific as “monovalent”. The term “valence” does not, however, correspond to the number of binding sites of the antibody. ...
... Polyspecific antibodies are sometimes incorrectly denoted as “polyvalent” and monospecific as “monovalent”. The term “valence” does not, however, correspond to the number of binding sites of the antibody. ...
Differential assembly of polypeptides of the light
... Figure 1 shows the near-IR absorption spectra of cell extracts and membrane fractions isolated after 0, 3, and 24 h of acclimation from high (1,100 W/m2) to low intensity illumination (100 W/m2). Only low levels of LH2 were present (LH2/LH1 molar ratio = 0.39) in the initial exponential-phase high l ...
... Figure 1 shows the near-IR absorption spectra of cell extracts and membrane fractions isolated after 0, 3, and 24 h of acclimation from high (1,100 W/m2) to low intensity illumination (100 W/m2). Only low levels of LH2 were present (LH2/LH1 molar ratio = 0.39) in the initial exponential-phase high l ...
Synthesis of RNA - Stamm revision
... coupling of ribonucleoside phosphoramidite building blocks on a solid support. The four steps of the synthesis cycle include: A) cleavage of the transient 5’-protecting group, B) activation of the phosphoramidite building block and coupling to the 5’-OH of the support bound nucleotide, C) capping of ...
... coupling of ribonucleoside phosphoramidite building blocks on a solid support. The four steps of the synthesis cycle include: A) cleavage of the transient 5’-protecting group, B) activation of the phosphoramidite building block and coupling to the 5’-OH of the support bound nucleotide, C) capping of ...
Identification and removal of colanic acid from plasmid DNA
... P Firozi1, W Zhang2,4, L Chen1, FA Quiocho2, KC Worley3 and NS Templeton1 ...
... P Firozi1, W Zhang2,4, L Chen1, FA Quiocho2, KC Worley3 and NS Templeton1 ...
Fundamentals and - 17th International Symposium on Chiral
... The lecture will introduce the phenomenon of chirality in a biological context, the chiral technologies in the industrial context and chiral discrimination by protein targets like receptors, enzymes and some examples of chiral discrimination in DNA. Prof. J. Gal Chirality As A Modulator Of Therapeut ...
... The lecture will introduce the phenomenon of chirality in a biological context, the chiral technologies in the industrial context and chiral discrimination by protein targets like receptors, enzymes and some examples of chiral discrimination in DNA. Prof. J. Gal Chirality As A Modulator Of Therapeut ...
Tetrahymena Contain Two Distinct and Unusual High Mobility Group
... the most thoroughly studied class of nonhistone chromosomal proteins. This family of relatively low molecular weight proteins (mol wt <30,000) can be extracted from chromatin by low salt or with dilute acids, and characteristically contain a large number of basic and acidic amino acids distributed i ...
... the most thoroughly studied class of nonhistone chromosomal proteins. This family of relatively low molecular weight proteins (mol wt <30,000) can be extracted from chromatin by low salt or with dilute acids, and characteristically contain a large number of basic and acidic amino acids distributed i ...
Gel electrophoresis
Gel electrophoresis is a method for separation and analysis of macromolecules (DNA, RNA and proteins) and their fragments, based on their size and charge. It is used in clinical chemistry to separate proteins by charge and/or size (IEF agarose, essentially size independent) and in biochemistry and molecular biology to separate a mixed population of DNA and RNA fragments by length, to estimate the size of DNA and RNA fragments or to separate proteins by charge.Nucleic acid molecules are separated by applying an electric field to move the negatively charged molecules through a matrix of agarose or other substances. Shorter molecules move faster and migrate farther than longer ones because shorter molecules migrate more easily through the pores of the gel. This phenomenon is called sieving.Proteins are separated by charge in agarose because the pores of the gel are too large to sieve proteins. Gel electrophoresis can also be used for separation of nanoparticles.Gel electrophoresis uses a gel as an anticonvective medium and/or sieving medium during electrophoresis, the movement of a charged particle in an electrical field. Gels suppress the thermal convection caused by application of the electric field, and can also act as a sieving medium, retarding the passage of molecules; gels can also simply serve to maintain the finished separation, so that a post electrophoresis stain can be applied. DNA Gel electrophoresis is usually performed for analytical purposes, often after amplification of DNA via PCR, but may be used as a preparative technique prior to use of other methods such as mass spectrometry, RFLP, PCR, cloning, DNA sequencing, or Southern blotting for further characterization.