Download 2960 Lab 2 NOTES

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Lab 2: Organelles,
Proteins, and SDS-PAGE
Dr. Sarah VanVickle-Chavez
BIO 2960 Laboratory
Spring 2013
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Overview
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Today we will load a gel and compare your gel to the sample gel. Each
person will load one lane on the gels in the center of each bench.
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While gels are running, we will do the PyMOL computer lab as a group
demo. You should have Lab Report 1a complete before we leave today.
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You can use your books and notes, but there is a brief computer
tutorial on the NSLC website to help you with Lab Report 1b.
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http://www.nslc.wustl.edu/courses/Bio2960/labs.html
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Next week, there are three reports due at the beginning of class
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1a = proteins (PyMOL demo in class)
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1b = cell organelles (use your book/notes for this one)
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2 = SDS-PAGE
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1. Protein Electrophoresis
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SDS-PAGE stands for sodium dodecyl sulfate polyacrylamide
gel electrophoresis
+ SDS vs Native Gel
How do you “see” your proteins?
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PyMOL
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Remember: Amino acids have different properties based on
the R-group
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GAVLIMPFW = nonpolar
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STNQYC = uncharged, polar
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KRHDE = charged, polar
+ Alpha helix
+ Beta Sheet