Download Biotech unit Objectives

Biotech unit Objectives
Chapters 19-21
Flex Day: January 18
Holiday: January 21
Chapter 19
Key Terms
Chromatin
Nucleosomes
DNA methylation
Control elements
Leucine zipper motif
Heterochromatin
DNA packaging
Histone acetylation
alternative splicing
BRCA gene
January 14- January 25
Test: Wednesday January 23
Ex days: January 24-25.
Euchromatin
satellite DNA
genomic imprinting
helix turn helix motif
proto-oncogenes
Protein scaffolding
immunoglobulins
apoptosis
zinc finger motif
tumor suppressor gene
Histones
antibody
enhancer
concogen
p53 gene
Objectives
 Describe how chromatin packing is accomplished and its significance.
 Be able to explain the methods of the cell repairing mutations.
 Be able to explain the key genes and processes in cancer cell formation.
Chapter 20
Key Terms
Plasmid
Thermocycler
Streak plate
DNA fingerprinting
Blunt ends
Gene library
Lambda DNA
Ti plasmid
Vector
Electrophoresis
transformation
DNA ligase
DNA fragments
probe
DNA sequencing
Microarrays
Genetic engineering
Gene therapy
Wells
Agarose gel
recombinant DNA
stem cells
RFLP analysis
sticky ends
restriction endonucleases hybridization
plasmid mapping
primer
tracking dye
lane marker
genetically modified foods electroporation
serial dilution
Polymerase Chain Reaction
transgenic organisms
colonies
cDNA
southern blotting
Sanger method
taq polymerase
Objectives
For the following techniques, be able to describe the technique and why it is used:
PCR
DNA sequencing (Sanger method)
DNA fingerprinting (RFLP analysis) with and without southern blotting
Genetic engineering
Creating a microarray assay
Electrophoresis
Identifying a cloned gene
Be able to map a plasmid using DNA fragment sizes produced by electrophoresis.
Understand the importance of
Restriction enzymes
Electrophoresis
DNA ligase
Agarose
Probes
primers
Tracking dye
Chapter 21
Key terms
Differentiation
Totpotency
Induction
apoptosis
Morphogenesis
Clone
Pattern formation
Homeobox
Pluripotent
Somites
Homeotic genes
Determination
Segmentation
Objectives
Be able to describe the steps on how to clone a mammal.
Be able to describe the terms above as they relate to the individual development of an organism.
Be able to explain how segmentation plays a critical role in the development of animals.
cell culture
Differentiation
maternal effect genes