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Lecture 12 - Gene expression Short Answer 1. What is gene expression 2. What is gene expression important Fill in the Blank a. Altering a protein to form an active final product _____ 1. First step in RNA splicing _____ 2. Second step in RNA splicing b. Action of inhibitors that may block synthesis of protein _____ 3. Alternative RNA splicing _____ 4. RNA interference _____ 5. Inhibition of translation _____ 6. Activation of finished protein c. Joining exons in different ways to produce more than one form mRNA message kind of mRNA ( and polypeptide ) from a single gene d. Removal of noncoding introns from RNA e. Joining of exons to produce mRNA f. Binding of microRNA (miRNA) to mRNA, blocking translation 1. ________________ are pieces of DNA that can relocate 2. ________________ also known as master genes 3. ________________ is something in addition do DNA that controls gene expression 4. Gene expression through attachment of acetyl groups to histones is know as ________________ 5. Insulin is an example of the importance of this process of gene expression know as _____________________ 6. The attachment of ________________ is a way to prevent gene expression 7. ______________ is when a X chromosome is randomly silenced 8. The process of removing introns and exons from an mRNA is an example of ___________________ ! a. homeotic genes b. jumping genes c. epigenetics d. protein folding e. RNA splicing f. Inactivation of X chromosome g. acetylation of histones h. cell signaling i. environment 1 Lecture 12 - Gene expression Multiple Choice 1. Your muscle and bone cells are different because a. they contain different sets of genes b. they are differentiated c. they contain different operons d. different genes are switched on and off in each e. they contain different histones 2. DNA packing - the way DNA is folded into chromosomes- affects gene expression by a. controlling access to DNA b. positioning related genes near each other c. protecting DNA from mutations d. enhancing recombination of genes e. allowing “unpacked” genes to be eliminated from the genome 3. After an mRNA molecule is transcribed from a eukaryotic gene, portions called _____ are removed and the remaining _______ are spliced together to produce an mRNA molecule with a continuous coding sequence a. operators....promoters b. exons....introns c. silencers....enhancers d. introns....exons e. promoters....operators 4. Which of the following mechanisms of gene regulation operates after mRnA transcription but before translation of mRNA into protein? a. mRNA splicing b. DNA packing c. repressors and activators d. protein degradation e. all of the above 5. Homeotic genes a. are responsible for the cellular changes that occur in cancer. b. coordinate development by controlling other genes c. are found only in certain cells d. are any genes transferred from cell to cell by viruses ! 2 Lecture 12 - Gene expression e. repair DNA and defend against mutations 6. In a eukarytoe, which of the following may block gene expression by binding to DNA? a. an operon b. a histone c. an enhancer d. a promoter e. a silencer 3 ! 3