* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download Exam Review 2B -- Rodermel
Transcription factor wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
Holliday junction wikipedia , lookup
Designer baby wikipedia , lookup
History of RNA biology wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Mitochondrial DNA wikipedia , lookup
DNA profiling wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Cancer epigenetics wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
SNP genotyping wikipedia , lookup
Genomic library wikipedia , lookup
Microevolution wikipedia , lookup
Genealogical DNA test wikipedia , lookup
DNA vaccination wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Microsatellite wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
Point mutation wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Molecular cloning wikipedia , lookup
Epigenomics wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
History of genetic engineering wikipedia , lookup
DNA polymerase wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
DNA replication wikipedia , lookup
Non-coding DNA wikipedia , lookup
Cre-Lox recombination wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Primary transcript wikipedia , lookup
Helitron (biology) wikipedia , lookup
BIO 313 Exam 2 Review B Supplemental Instruction Iowa State University Leader: Course: Instructor: Date: Laura Bio 313 Dr. Rodermel 9/30/15 1. What modes of replication are seen with circular DNA but not linear DNA? 2. Diagram Rolling Circle replication below. Include the 3 different products that can result. (Be sure to include leading and lagging strand, origin of replication, directionality of the strands) 3. Match the enzyme to its function: Initiator Protein Moves ahead of replication fork, making and resealing breaks in order to release build up of torque DNA Helicase Elongates a new nucleotide strand from the 3’-OH group provided by the primer Single-Strand-Binding Proteins Binds to origin and separates strands of DNA to initiate transcription DNA Gyrase Removes RNA primers and replaces them with DNA DNA Primase DNA Polymerase III Joins Okazaki fragments by sealing breaks in the sugar-phosphate backbone of newly synthesized DNA Attach to single-stranded DNA and prevent secondary structures from forming Unwinds DNA at replication fork DNA Polymerase I DNA ligase Synthesizes a short RNA primer to provide a 3’-OH group for the attachment of nucleotides 1060 Hixson-Lied Student Success Center 515-294-6624 [email protected] http://www.si.iastate.edu 4. What is required in order for elongation to occur in replication? 5. What is DNA licensing? How does the cell control DNA licensing? 6. Explain the end replication problem and why not all DNA have this problem: 7. If the gene for Primase were mutated, resulting in no functional primase, what would be the effect on theta, rolling circle and linear replication? 8. Describe how you would perform an experiment to test the hypothesis that shorter telomeres results in aging: 9. Draw the cell cycle (Be sure to include checkpoints) 10. What is the primary and secondary structure of RNA? 11. Draw a diagram of transcription: 12. RNA polymerases carry out transcription at a much slower rate than that at which DNA polymerases carry out replication. Why is speed more important in replication than in transcription? 13. What are the two important consensus sequences in bacterial transcription? What about eukaryotic transcription? 14. Explain how sigma acts and why sigma is so important? 15. What is Rho independent termination? (Draw a diagram to help explain) 16. What does it mean to be co-linear? Why are eukaryotic genes NOT co-linear? 17. What are the four types of introns? a. b. c. d. 18. What is the function of the 5’-cap? (3 things) 19. What is the consensus sequence for 3’- cleavage during modification? 20. What is a splicesome? 21. BACTERIAL 22. EUKARYOTIC