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LOYOLA COLLEGE (AUTONOMOUS), CHENNAI – 600 034 M.Sc. DEGREE EXAMINATION – FOOD CHEMISTRY SECOND SEMESTER – April 2009 WD 59 FP 2801 - NUTRITIONAL BIOCHEMISTRY Date & Time: 22/04/2009 / 1:00 - 4:00 Dept. No. Max. : 100 Marks PART-A Answer all the questions: (10 x 2 = 20) 1. a) The small fragments of DNA, produced during replication are called as --------------b) The enzyme catalyzing the synthesis of RNA from DNA is --------------------. 2. Give the structure of RNA polymerase. 3. Name the two antibiotics which act as ionophores for potassium (k+) ions. 4. What is the role of uncouplers in Electron transport chain? 5. What are the different types of ketone bodies? Give their structure. 6. What is alkaptanuria and fructosuria? 7. What is the role of topoisomerase in DNA replication? 8. What is the role of antidiuretic hormone in water balance? 9. What is insensible water loss? 10. Bring out the difference between diabetes insipidus and diabetes mellitus. PART-B Answer any eight questions: (8 x 5 = 40) 11. Explain the role of inhibitors in oxidative phosphorylation. 12. Describe any two inborn errors of carbohydrate metabolism. 13. Explain the role of different types of complexes involved in electron transport chain. 14. Explain the biosynthesis of saturated fatty acids. 15. Describe the Watson and Crick model of DNA. 16. Explain diagrammatically how the replication gets terminated in the prokaryotes. 17. Explain any two DNA repair mechanisms. 18. Explain the biosynthesis of ketone bodies. 19. Describe the post transcriptional modification of mRNA. 20. What is over hydration and dehydration? 21. What are the disorders of acid base balance? 22. Describe the Hamberger’s phenomenon. PART C Answer any four questions (4 x 10 = 40) 23. Describe oxidative phosphorylation. 24. Give an account on transcription in prokaryotes. 25. Justify- ‘the citric acid cycle is the final common metabolic pathway for the oxidation of nutrients’. 26. Explain cholesterol biosynthesis. 27. Explain in detail how the normal pH of the blood is maintained? 28. Explain the biochemistry of the digestion of carbohydrates and proteins. ***************