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BIOL 3301
GENETICS
Date: Monday, March 10, 1.00-2.30 pm
Subject: The structure and replication of DNA.
Reading in ‘An introduction to genetic analysis’ (Griffiths et al., 7th edition)
Chapter 8: The structure and replication of DNA.
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Key concepts and keywords:
DNA: the genetic material.
transformation
Griffith/ Avery, MacLeod, Mc Carty/ Hershey and Chase (8-1, 8-3)
Structure of DNA.
nucleotide, nucleoside, base (purine & pyrimidine) (8-4)
Chargaff’s rules
double helix: phosphate backbone, hydrogen bonds, antiparallel, A-DNA, B-DNA, ZDNA, major and minor grooves (8-5, 8-6, 8-8, 8-9)
Replication of DNA.
semiconservative (8-10)
Meselson & Stahl experiment
Mechanisms of DNA replication.
DNA polymerases
Origin and terminus of replication (8-22, 8-23)
Priming DNA synthesis (8-20)
Leading and lagging strand (8-28)
Okazaki fragment (8-30)
Replication at chromosome tips, telomerase (8-32)
Helicases and topoisomerases
Exonuclease editing: proofreading (8-38)
Experimental applications of base-sequence complementarity
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Homework:
Problems 1, 12, 13
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Reading for next class (3-17) :
Chapter 9: Genetics of DNA function.