Download Exam Review 2B -- Rodermel

BIO 313 Exam 2 Review B
Supplemental Instruction
Iowa State University
Leader:
Course:
Instructor:
Date:
Laura
Bio 313
Dr. Rodermel
9/30/15
1. What modes of replication are seen with circular DNA but not linear DNA?
2. Diagram Rolling Circle replication below. Include the 3 different products that can result.
(Be sure to include leading and lagging strand, origin of replication, directionality of the
strands)
3. Match the enzyme to its function:
Initiator Protein
Moves ahead of replication fork, making and
resealing breaks in order to release build up
of torque
DNA Helicase
Elongates a new nucleotide strand from the
3’-OH group provided by the primer
Single-Strand-Binding Proteins
Binds to origin and separates strands of DNA
to initiate transcription
DNA Gyrase
Removes RNA primers and replaces them
with DNA
DNA Primase
DNA Polymerase III
Joins Okazaki fragments by sealing breaks in
the sugar-phosphate backbone of newly
synthesized DNA
Attach to single-stranded DNA and prevent
secondary structures from forming
Unwinds DNA at replication fork
DNA Polymerase I
DNA ligase
Synthesizes a short RNA primer to provide a
3’-OH group for the attachment of
nucleotides
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4. What is required in order for elongation to occur in replication?
5. What is DNA licensing? How does the cell control DNA licensing?
6. Explain the end replication problem and why not all DNA have this problem:
7. If the gene for Primase were mutated, resulting in no functional primase, what would be
the effect on theta, rolling circle and linear replication?
8. Describe how you would perform an experiment to test the hypothesis that shorter
telomeres results in aging:
9. Draw the cell cycle (Be sure to include checkpoints)
10. What is the primary and secondary structure of RNA?
11. Draw a diagram of transcription:
12. RNA polymerases carry out transcription at a much slower rate than that at which DNA
polymerases carry out replication. Why is speed more important in replication than in
transcription?
13. What are the two important consensus sequences in bacterial transcription? What about
eukaryotic transcription?
14. Explain how sigma acts and why sigma is so important?
15. What is Rho independent termination? (Draw a diagram to help explain)
16. What does it mean to be co-linear? Why are eukaryotic genes NOT co-linear?
17. What are the four types of introns?
a.
b.
c.
d.
18. What is the function of the 5’-cap? (3 things)
19. What is the consensus sequence for 3’- cleavage during modification?
20. What is a splicesome?
21. BACTERIAL
22. EUKARYOTIC