Survey
* Your assessment is very important for improving the workof artificial intelligence, which forms the content of this project
Download the element makes na RNA copy of itself which is reversed
Document related concepts
DNA barcoding wikipedia , lookup
Mitochondrial DNA wikipedia , lookup
Copy-number variation wikipedia , lookup
Nutriepigenomics wikipedia , lookup
Comparative genomic hybridization wikipedia , lookup
DNA profiling wikipedia , lookup
Designer baby wikipedia , lookup
Cancer epigenetics wikipedia , lookup
Point mutation wikipedia , lookup
DNA polymerase wikipedia , lookup
Genomic library wikipedia , lookup
Transposable element wikipedia , lookup
Bisulfite sequencing wikipedia , lookup
Zinc finger nuclease wikipedia , lookup
DNA vaccination wikipedia , lookup
SNP genotyping wikipedia , lookup
Genealogical DNA test wikipedia , lookup
DNA damage theory of aging wikipedia , lookup
Gel electrophoresis of nucleic acids wikipedia , lookup
Primary transcript wikipedia , lookup
United Kingdom National DNA Database wikipedia , lookup
Genetic engineering wikipedia , lookup
Cell-free fetal DNA wikipedia , lookup
Epigenomics wikipedia , lookup
Vectors in gene therapy wikipedia , lookup
Molecular cloning wikipedia , lookup
Non-coding DNA wikipedia , lookup
Extrachromosomal DNA wikipedia , lookup
DNA supercoil wikipedia , lookup
Microevolution wikipedia , lookup
Artificial gene synthesis wikipedia , lookup
Nucleic acid double helix wikipedia , lookup
Genome editing wikipedia , lookup
No-SCAR (Scarless Cas9 Assisted Recombineering) Genome Editing wikipedia , lookup
Nucleic acid analogue wikipedia , lookup
Holliday junction wikipedia , lookup
History of genetic engineering wikipedia , lookup
Therapeutic gene modulation wikipedia , lookup
Homologous recombination wikipedia , lookup
Site-specific recombinase technology wikipedia , lookup
Deoxyribozyme wikipedia , lookup
Transcript
Replicação na presença de erros Recombination at the Molecular Level • Breakage and joining also directed by enzymes. • Homologous recombination occurs during synapsis in meiosis I, general recombination in bacteria, and viral genetic exchange. • Molecular mechanism proposed by Holliday and Whitehouse (1964). • Depends on complementary base pairing. RecA protein-mediated DNA synapsis Rec A has multiple DNA binding sites, hence can hold a single strand and a double helix together Rec A is also a DNA-dependent ATPase Recombinational DNA repair Site-specific recombination Integração ou excisão de um bacteriofago Conservative transposition – the element itself moves from the donor site into the target site Replicative transposition – the element moves a copy of itself to a new site via a DNA intermediate Retrotransposition – the element makes na RNA copy of itself which is reversed transcribed into a DNA which is then inserted When IS elements get it together: composite transposons Tn10 (E.coli) Two IS elements flank an antibiotic resistance gene
