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Transcript
Let’s Find the Pheromone Gene DNA Transcription Animation Every cell has 2 complete DNA copies, but cells in different body regions express different genes. QuickTime™ and a QuickTime™ and a TIFF (Uncompressed) decompressor TIFF (Uncompressed) decompressor are needed to seetothis are needed see picture. this picture. Genes transcribed for neural development and antennae Genes transcribed for legs and muscle movements Genes transcribed for digestion and reproduction Where do you think the pheromone message is produced? Head ? Thorax ? Abdomen ? Where do you think the pheromone gene is expressed? Problem: DNA is a tiny molecule PCR = DNA Copier mRNA DNA PCR Animation PCR on the Termites H Head T A Controls 1. Negative 2. Positive Thorax Abdomen Ladder Using primers for pheromone-related gene What are primers? AGTAACTCTTGAGCCG CGTTAGCGATTCGGTCG Short but unique stretches of DNA that act recognize and bind to the “address” for our target pheromone’s message in the cell http://dna.chromosome12.termite.org/pheromonegene.html Looking for the Gene - H T A - + L + Gel Electrophoreses Gel Animation How do we do it? 1. Get pre-poured gels and remove tape 2. Using pipettor, fill wells with 5uL of Head, Thorax, and Abdomen PCR products as well as the controls and the ladder 3. Molecular Technician puts gel in the buffer-filled box and starts the electrical charge (RUN TO RED! DNA is negative and runs to the positive charge.) Let the gel run for 10 minutes 4. CAREFULLY bring the gel to the Ethidium Bromide and give it to a graduate student to soak for you (12 minutes) 5. Gel will be put on the UV light so you can see where your gene amplified! Your Gel What does it mean? Thank you for coming! • Any questions about today’s experiments? • Please finish filling out your worksheets and hand them to your teacher. • Please fill out evaluations to let us know about your experience! • PIZZA downstairs!
