Download PCR/GMO Worksheet

GMO-PCR Worksheet
Name_________________
1. What materials are necessary to place in a PCR reaction? How are primers
designed to assure that a specific gene (or DNA sequence) is amplified?
2. Pls. explain the purpose of each temperature step for the PCR reaction.
a. 95 C
b. 55-60 C
c. 72 C
3. When doing a PCR reaction, please explain why we do 30-40 cycles of each of the
steps above?
4. When doing agarose gel electrophoresis, why do we subject our gel to electrical
current? What properties of the buffer allow us to run current through it?
5. Which electrode will the DNA run towards? Why is this the case?
6. What would you have expected to see after you ran your gel if the fruit/vegetable
is genetically modified? What did you actually see?
7. Pls. affix a copy of your gel picture to the back of this worksheet. Pls. label each
lane. Pls. also estimate the size of the DNA band(s) that you see in each of your PCR
lanes