Download Let`s Find the Pheromone Gene

Let’s Find the Pheromone Gene
DNA Transcription
Animation
Every cell has 2 complete DNA copies, but
cells in different body regions express
different genes.
QuickTime™
and a
QuickTime™ and a
TIFF (Uncompressed)
decompressor
TIFF (Uncompressed) decompressor
are needed
to seetothis
are needed
see picture.
this picture.
Genes transcribed for neural
development and antennae
Genes transcribed for legs
and muscle movements
Genes transcribed for
digestion and reproduction
Where do you think the pheromone message is
produced?
Head ?
Thorax ?
Abdomen ?
Where do you think the pheromone gene is
expressed?
Problem: DNA is a tiny molecule
PCR = DNA Copier
mRNA
DNA
PCR
Animation
PCR on the Termites
H
Head
T
A
Controls
1.
Negative
2.
Positive
Thorax
Abdomen
Ladder
Using primers for pheromone-related gene
What are primers?
AGTAACTCTTGAGCCG
CGTTAGCGATTCGGTCG
Short but unique stretches of DNA that act recognize and bind
to the “address” for our target pheromone’s message in the
cell
http://dna.chromosome12.termite.org/pheromonegene.html
Looking for the Gene
-
H
T
A
-
+
L
+
Gel Electrophoreses
Gel Animation
How do we do it?
1. Get pre-poured gels and remove tape
2. Using pipettor, fill wells with 5uL of Head, Thorax, and
Abdomen PCR products as well as the controls and the
ladder
3. Molecular Technician puts gel in the buffer-filled box and
starts the electrical charge (RUN TO RED! DNA is
negative and runs to the positive charge.) Let the gel run
for 10 minutes
4. CAREFULLY bring the gel to the Ethidium Bromide and
give it to a graduate student to soak for you (12 minutes)
5. Gel will be put on the UV light so you can see where your
gene amplified!
Your Gel
What does it mean?
Thank you for coming!
• Any questions about today’s
experiments?
• Please finish filling out your worksheets
and hand them to your teacher.
• Please fill out evaluations to let us know
about your experience!
• PIZZA downstairs!