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Transcript
FROM GENE TO PROTEIN PART 2 Goal 1- Understand the process of transcription • How is RNA made? •How to get from DNA to RNA Goal 2- Demonstrate how RNA is made • Transcription- Model activity TRY THIS! Molecular Genetics Alphabet Online Activity • Crack this code DNA nucleotide triplet ACG Cys RECAP: FROM GENE TO PROTEIN PART 1 What are the two main processes linking gene to protein? How RNA is made? (make a prediction) What might the cell need/use to copy/transcribe DNA? “Cookbook” (genotype) “Copy of recipe” “Dish” (phenotype) TRANSCRIPTION: RNA SYNTHESIS 3 Phases: Initiation (start) Elongation (strand) Termination (stop) To make a copy you need 3 things: 1.Something to copy – DNA! (cookbook) 2.Something to copy with – Nucleotides! (ingredients) 3.Something to make the copy – Enzymes! (recipe) INITIATION (START) Goal: to express (turn on) Green fluorescent protein DNA nucleotides 3’ 5’ DNA template (the “something” to copy) DNA nucleotides 3’ 5’ 1. DNA template (the something to copy) 2. RNA nucleotides (the something to copy with) 3. RNA polymerase enzyme (the something to make the copy) Transcription factor Green fluorescent gene Promoter region Termination sequence 5’ 3’ Nucleotides (T-T-A-T-T-T) “TA-TA” box Transcription unit “Downstream” “Upstream” Bind/unwind DNA ELONGATION (STRAND) Green fluorescent gene 3’ 5’ ription tor 3’ 5’ Direction of RNA synthesis 5’ 3’ Termination (stop) Green fluorescent gene 3’ 5’ 5’ Transcription unit Termination sequence Transcribed (copied) 3 ’ messenger RNA (mRNA) Green fluorescent protein Translated TRANSCRIPTION VIDEOS mRNA synthesis Stages of Transcription
